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http://dx.doi.org/10.5352/JLS.2008.18.3.418

Properties of the Membrane-Bound NADH;Menadione Oxidoreductase in the Aerobic Respiratory Chain of Bacillus cereus  

Kang, Ji-Won (Department of Microbiology, Changwon National University)
Kim, Young-Jae (Department of Microbiology, Changwon National University)
Publication Information
Journal of Life Science / v.18, no.3, 2008 , pp. 418-421 More about this Journal
Abstract
Membranes prepared from Bacillus cereus KCTC 3674, grown aerobically on a complex medium, oxidized NADH exclusively, whereas deamino-NADH was little oxidized. The respiratory chain-linked NADH oxidase system exhibited an apparent $K_m$ value of approximately 65 ${\mu}M$ for NADH. On the other hand, the enzymatic properties of the NADH: menadione oxidoreductase of NADH oxidase system were examined. The maximum activity of NADH: menadione oxidoreductase was obtained at pH 9.5 in the presence of 0.1 M KCl (or NaCl). The NADH: menadione oxidoreductase activity was very resistant to the respiratory chain inhibitors such as rotenone, capsaicin, and $AgNO_3$. Interestingly, the activity was stimulated by the 2-heptyl-4-hydroxyquinoline-N-oxide (HQNO).
Keywords
Bacillus cereus KCTC 3674; aerobic respiratory chain; NADH: menadione oxidoreductase;
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1 Yagi, T., T. Yano, S. D. Bernardo and A. Matsuno-Yagi. 1998. Procaryotic complex I (NDH-1), an overview. Biochim. Biophys. Acta 1364, 125-133.   DOI   ScienceOn
2 Matsushita, K., T. Ohnishi and R. Kaback. 1987. NADH: ubiquinone oxidoreductase of the Escherichia coli aerobic respiratory chain. Biochemistry 26, 7732-7737.   DOI   ScienceOn
3 Tokuda, H. 1983. Isolation of Vibrio alginolyticus mutants defective in the respiration-coupled $Na^+$ pump. Biochem. Biophys. Res. Commun. 114, 113-118.   DOI   ScienceOn
4 Tokuda, H. and T. Unemoto. 1984. $Na^+$ is translocated at NADH: quinone oxidoreductase segment in the respiratory chain of Vibrio alginolyticus. J. Biol. Chem. 259, 7785- 7790.
5 Yagi, T. 1990. Inhibition by capsaicin of NADH-quinone oxidoreductases is correlated with the presence of energy- coupling site 1 in various organisms. Arch. Biochem. Biophys. 281, 305-311.   DOI   ScienceOn
6 Kim, S. S., Y. Park, J. Lee, J. Yoon, Y. Shin, I. Rhee and Y. J. Kim. 1998. Taxonomic studies of the beta hemolysis- causing pathogen Bacillus cereus isolated from sea water. J. Microbiol. Biotech. 8, 67-73.   과학기술학회마을
7 Kim, S. S., Y. Park, I. Rhee, and Y. J. Kim. 2000. Influence of temperature, oxygen, m-chlorophenylhydrazone, cerulenin, and quinacrine on the production of extracellular proteases in Bacillus cereus. J. Microbiol. Biotech. 10, 103-106.   과학기술학회마을
8 Kim, Y. J., S. Mizushima and H. Tokuda. 1991. Fluorescence quenching studies on the characterization of energy generated at the NADH: quinone oxidoreductase and quinol oxidase segments of marine bacteria. J. Biochem. (Tokyo) 109, 616-621.   DOI
9 Kim, Y. J., K. Song and S. Rhee. 1995. A novel aerobic respiratory chain-linked NADH oxidase system in Zymomonas mobilis. J. Bacteriol. 177, 5176-5178.   DOI
10 Asano, M., M. Hayashi, T. Unemoto and H. Tokuda. 1985.$Ag^+$-sensitive NADH dehydrogenase in the $Na^+$-motive respiratory chain of the marine bacterium Vibrio alginolyticus. Agric. Biol. Chem. (Biosci. Biotechnol. Biochem.) 49, 2813-2817.   DOI
11 Collins, M. D. and D. Jones. 1981. Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiol. Rev. 45, 316-354.
12 Kim, S. S., Y. J. Kim and I. Rhee. 2001. Purification and characterization of anovel extracellular protease from Bacillus cereus KCTC 3674. Arch. Microbiol. 175, 458-461.   DOI   ScienceOn
13 Yagi, T., K. Hoh-nami and T. Ohnishi. 1988. Purification and characterization of two types of NADH-quinone reductase from Thermus thermophilus HB-8. Biochemistry 27, 2008-2013.   DOI   ScienceOn