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http://dx.doi.org/10.5352/JLS.2008.18.3.403

Effect of Ethanol Extract from Peel of Citrus junos and Poncirus trifoliata on Antioxidant and Immune Activity.  

Park, Joon-Hee (Department of Molecular Biology, Dong-Eui University)
Kang, Byoung-Won (BK21 Center for Silver-Bio industrialization Project, Dong-A University)
Kim, Ji-Eun (Department of Biotechnology, Dong-A University)
Seo, Min-Jeong (Department of Biotechnology, Dong-A University)
Lee, Young-Choon (Department of Biotechnology, Dong-A University)
Lee, Jai-Heon (Department of Genetic Engineering, Dong-A University)
Joo, Woo-Hong (Department of Biology, Changwon National University)
Choi, Yung-Hyun (Department of Biochemistry, College of Oriental Medicine, Dong-eui University)
Lim, Hak-Seob (Department Bioinstitute, MILLENNIUM PROMISE CO., LTD.)
Jeong, Yong-Kee (Department of Biotechnology, Dong-A University, Department Bioinstitute, MILLENNIUM PROMISE CO., LTD.)
Lee, Bok-Kyu (Department of Molecular Biology, Dong-Eui University)
Publication Information
Journal of Life Science / v.18, no.3, 2008 , pp. 403-408 More about this Journal
Abstract
In this study, we compared with 80% ethanol extracts from peel of Poncirus trifoliata (PTP) and peel of Citrus junos (CJP) against antioxidant and immune activities. Total phenolics and flavonoids contents in PTP extracts were $60.75{\pm}1.15$ and $33.75{\pm}0.15$ mg/l00 g, respectively, and those were lower than CJP extracts. Antioxidant activities of PTP were increased with the more concentration, and were similar to CJP. Antioxidant activities of PTP were increased with increasing of concentration, and were similar to those of CJP. The NO production in macrophage cell lines were increased in a dose-dependent manner, until 5 mg/ml of CJP and 1 mg/ml of PTP compared with control cells, but decreased at higher concentrations. The proliferation of mouse spleen cells were increased in a dose-dependent manner, until 1 mg/ml of CJP and PTP compared with control cells but decreased at higher concentrations. The NO production in macrophage cell lines treated with PTP and CJP were increased in a dose-dependent manner, compared with untreated control cells until the concentrations of $1{\sim}5$ mg/ml (CJP) and 1 mg/ml (PTP) but decreased at higher concentrations than that. The proliferation of mouse spleen cells treated with PTP and CJP were increased in a dose-dependent manner, compared with untreated control cells until the concentration of 1 mg/ml but decreased at higher concentrations than that.
Keywords
Citrus junos; Poncirus trifoliata; antioxidant activity; immune activity;
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