Browse > Article
http://dx.doi.org/10.5352/JLS.2008.18.3.304

Overexpression of NtHSP70-1 Protects Chlorophyll from High Temperature in Plants  

Cho, Eun-Kyung (Department of Bio-Food Materials, College of Medical Life Sciences, Silla University)
Hong, Choo-Bong (Institute of Molecular Biology and Genetics and School of Biological Sciences, Seoul National University)
Publication Information
Journal of Life Science / v.18, no.3, 2008 , pp. 304-310 More about this Journal
Abstract
Heat shock protein 70 (HSP70) is known as molecular chaperone, the fundamental protein participating in various processes, from nascent protein synthesis to protection of proteins during abiotic stresses and developmental programs. However, their biological functions in plants are not yet well known. Here, NtHSP70-1 (AY372069), HSP70 of Nicotiana tabacum induced by heat stress was investigated. To analyze the protective role of NtHSP70-1, transgenic tobacco plants, which constitutively overexpressed NtHSP70-1 as well as contained either the vector alone or having NtHSP70-1 in the antisense orientation, were constructed. The altered NtHSP70-1 levels in plants were confirmed by western blotting and transgenic sense lines exhibited tolerance to heat stress. Seedlings with the constitutively expressed NtHSP70-1 grew as green or healthy plants after heat stress. In contrast, transgenic vector or antisense lines exhibited yellowing of leaves or some delay in growth, which finally led to death. Evaluation of chlorophyll contents of heat-shocked transgenic tobacco seedlings indicated that NtHSP70-1 contributes to thermotolerance by preventing chlorophyll synthesis in plants.
Keywords
NtHSP70-1; chlorophyll; transgenic tobacco plant; thermotolerance;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Zer, H., O. Prasil and I. Ohad. 1994. Role of plastoquinol oxidoreduction in regulation of photochemical reaction center II D1 protein turnover in vivo. J. Biol. Chem. 269, 17670- 17676.
2 von Elbe, J. H. and S. J. Schwartz. 1996. Colorants. pp. 651-722, In Fennema, O. R. (ed.), Food Chem. Marcel Dekker Inc., New York.
3 Willows, R. D. 2003. Biosynthesis of chlorophylls from protoporphyrin IX. Nat. Prod. Rep. 20, 327-341.   DOI   ScienceOn
4 Sweeney, J. P., and M. E. Martin. 1961. Stability of chlorophyll in vegetables as affected by pH. Food Tech. 15, 263-266.
5 Suh, M. C., C. B. Hong, S. S. Kim and W. S. Sim. 1994. Transgenic tobacco plants with Bacillus thuringiensis delta- toxin gene resistant to Korean born tobacco budworms. Mol. Cells 4, 211-219.
6 Schwartz, S. J. and J. H. von Elbe, 1983. Kinetics of chlorophyll degradation to pyropheophytin in vegetables. J. Food Sci. 48, 1303-1306   DOI
7 Vavilin, D. V. and W. F. Vermaas. 2002. Regulation of the tetrapyrrole biosynthetic pathway leading to heme and chlorophyll in plants and cyanobacteria. Physiol. Plant 115, 9-24.   DOI   ScienceOn
8 Schuster, G., R. Timberg and I. Ohad. 1988. Turnover of thylakoid photosystem II proteins during photoinhibition of Chlamydomonas reinhardtii. Eur. J. Biochem. 177, 403-410.   DOI   ScienceOn
9 Tanaka, A. and R. Tanaka. 2006. Chlorophyll metabolism. Curr. Opin. Plant Biol. 9, 248-255.   DOI   ScienceOn
10 Tijkens, L. M. M., S. A. Barringer and E. S. A. Biekman. 2001. Modelling the effect of pH on the colour degradation of blanched broccoli. Innov. Food Sci. Emer. Tech. 2, 315-322.   DOI   ScienceOn
11 Zer, H. and I. Ohad. 1995. Photoinactivation of photosystem II induces changes in the photochemical reaction center II abolishing the regulatory role of the QB site in the D1 protein degradation. Eur. J. Biochem. 231, 448-453.   DOI   ScienceOn
12 Andersson, B. and J. Barber. 1994. Molecular processes in photosynthesis. Adv. Mol. Cell Biol. 10, 1-53.   DOI
13 Schroda, M., J. Kropat, U. Oster, W. Rdiger, O. Vallon, F. A. Wollman, C. F. Beck. 2001. A role for molecular chaperones in assembly and repair of photosystem II. Biochem. Soc. Trans. 29, 413-418.   DOI   ScienceOn
14 Li, Q. B., D. Haskell, C. Zhang, D. Y. Sung and C. Guy. 2000. Diurnal regulation of Hsp70s in leaf tissue. Plant J. 21, 373-378.   DOI   ScienceOn
15 Luft, J. C. and D. J. Dix. 1999. Hsp70 expression and function during embryogenesis. Cell Stress Chaperon 4, 162-170.   DOI   ScienceOn
16 Oh, S. A., J. H. Park, G. I. Lee, K. H. Paek, S. K. Park andH. G. Nam. 1997. Identification of three genetic loci controlling leaf senescence in Arabidopsis thaliana. Plant J. 12, 527-535.   DOI   ScienceOn
17 Papenbrock, J., H. P. Mock, R., Tanaka, E. Kruse and B. Grimm. 2000. Role of magnesium chelatase activity in the early steps of the tetrapyrrole biosynthetic pathway. Plant Physiol. 122, 1161-1169.   DOI
18 Ohad, I., N. Keren, H. Zer, H. Gong, T. S. Mor, A. Gal, S. Tal and Y. Domovich. 1994. Light-induced degradation of the Photosystem II reaction centre D1 protein in vivo: an integrated approach, pp. 161-178, In Baker, N. R. and J. R. Bowyer (eds.), Photoinhibition Photosynth.: From Mol. Mechanisms to Field, Oxford: BIOS Scientific Publishers.
19 Melis, A. 1991. Dynamics of photosynthetic membrane composition and function. Biochim. Biophys. Acta. 1058, 87-106.   DOI   ScienceOn
20 Ohad, I., D. J. Kyle and J. Arntzen. 1984. Membrane protein damage and repair: removal and replacement of inactivated 32-kilodalton polypeptides in chloroplast membranes. J. Cell Biol. 99, 481-485.   DOI   ScienceOn
21 Sambrook, J., E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor., New York.
22 Schnell, D. J., F. Kessler and G. Blobel. 1994. Isolation of components of the chloroplast protein import machinery. Science 266, 1007-1012.   DOI
23 Schwartz, S. J. and T. V. Lorenzo. 1991. Chlorophyll stability during continuous aseptic processing and storage. J. Food Sci. 56, 1059-1062.   DOI
24 Schuster, G., D. Even, K. Kloppstech, and I. Ohad. 1988. Evidence for protection by heat-shock proteins against photoinhibition during heat-shock. J. Eur. Mol. Biol. Organ. 7, 1-6.
25 Krebs, R. A. and M. E. Feder. 1997. Negative consequences of Hsp70 overexpression in Drosophila melanogaster larvae. Cell Stress Chaperon 2, 60-71.   DOI   ScienceOn
26 Schroda, M., O. Vallon, F. A. Wollman, and C. F. Beck. 1999. A chloroplast-targeted heat shock protein 70 (HSP70) contributes to the photoprotection and repair of photosystem II during and after photoinhibition. Plant Cell 11, 1165-1178.   DOI   ScienceOn
27 Cho, E. K. and C. B. Hong. 2006. Over-expression of tobacco NtHSP70-1 contributes to drought-stress tolerance in plants. Plant Cell Rep. 25, 349-358   DOI   ScienceOn
28 Canjura, F. L., S. J. Schwartz and R. V. Nunes. 1991. Degradation kinetics of chlorophylls and chlorophyllides. J. Food Sci. 56, 1639-1643.   DOI
29 Koca, N., F. Karadeniz and H. S. Burdurlu. 2007. Effect of pH on chlorophyll degradation and colour loss in blanched green peas. Food Chem. 100, 609-615.   DOI   ScienceOn
30 Ko, K., O. Bornemisza, L. Kourtz, Z. W. Ko, W. C. Plaxton and A. R. Cashmore. 1992. Isolation and characterization of a cDNA clone encoding a cognate 70 kDa heat shock protein of the chloroplast envelope. J. Biol. Chem. 267, 2986-2993.
31 Hortensteiner, S., K. L. Wuthrich, P. Matile, K. H. Ongania and B. Krautler. 1998. The key step in chlorophyll breakdown in higher plants: cleavage of pheophorbide a macrocycle by a monooxygenase. J. Biol. Chem. 273, 15335-15339.   DOI   ScienceOn
32 Kropat, J., U. Oster, W. Rue digger and C. F. Beck. 2000. Chloroplast signalling in the light induction of nuclear HSP70 genes requires the accumulation of chlorophyll precursors and the export of these compounds to the cytoplasm/ nucleus. Plant J. 24, 523-531.   DOI   ScienceOn
33 Buchanan, M., L. Starrs, S. U. Egelhaaf and M. E. Cates. 2000. Kinetic pathways of multiphase surfactant systems. Phys. Rev. E 62, 6895-6905.
34 Kropat, J., U. Oster, W. Rue diger and C. F. Beck. 1997. Chlorophyll precursors are signals of chloroplast origin involved in light induction of nuclear heat-shock genes. Proc. Natl. Acad. Sci. USA. 94, 14168-14172.   DOI   ScienceOn
35 Beale, S. I. 1999. Enzymes of chlorophyll biosynthesis. Photosyn. Res. 60, 43-73.   DOI   ScienceOn
36 Aro, E. M., I. Virgin and B. Andersson. 1993. Photoinhibition of photosystem II. Inactivation, protein damage and turnover. Biochim. Biophys. Acta. 1143, 113-134.   DOI   ScienceOn
37 Heaton, J. W. and A. G. Marangoni. 1996. Chlorophyll degradation in processed foods and senescent plant tissues. Trends Food Sci. Tech. 7, 8-15.   DOI   ScienceOn
38 Grossman, A. R., M. Lohr and C. S. Im. 2004. Chlamydomonas reinhardtii in the landscape of pigments. Annu. Rev. Genet. 38, 119-173.   DOI   ScienceOn
39 Eckhardt, U., B. Grimm and S. Hortensteiner. 2004. Recent advances in chlorophyll biosynthesis and breakdown in higher plants. Plant Mol. Biol. 56, 1-14.   DOI   ScienceOn
40 Dix, D. J. 1997. Hsp70 expression and function during gametogenesis. Cell Stress Chaperon 2, 73-77.   DOI   ScienceOn