Browse > Article
http://dx.doi.org/10.5352/JLS.2008.18.2.180

Inhibition of Phorbol 12-myristate-13-acetate Induced Cyclooxygenase-2 Activity by Three-step Fermented Soybeans  

Park, Cheol (Department of Biochemistry, Dongeui University College of Oriental Medicine)
Lee, Jeong-Ok (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University)
Ryu, Chung-Ho (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University)
Choi, Yung-Hyun (Department of Biochemistry, Dongeui University College of Oriental Medicine)
Publication Information
Journal of Life Science / v.18, no.2, 2008 , pp. 180-186 More about this Journal
Abstract
In this study, we examined the effects of the fermented soybeans by Bacillus subtilis (FSB) and the novel three-step fermented soybeans (TFS) on the expression and activity of COX-2 in human leukemic U937 cell model. Treatment of phorbol 12-myristate 13-acetate (PMA) significantly induced pro-inflammatory mediators such as COX-2 expression and prostaglandin $E_2\;(PGE_2)$ production, whereas the levels of COX-1 remained unchanged. However, pre-treatment with FSB and TFS significantly attenuated the PMA-induced COX-2 protein as well as mRNA, which was associated with inhibition of $PGE_2$ production. Moreover, TFS exerts a much better inhibitory activity than FSB against PMA-induced activation of COX-2 and production of $PGE_2$ in U937 cells. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-inflammatory activity of FSB and TFS.
Keywords
Cyclooxygenase-2; prostaglandin $E_2$; fermented soybeans by Bacillus subtilis; three-step fermented soybeans;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Heuze-Vourc'h, N., M. Liu, H. Dalwadi, F. E. Baratelli, L. Zhu, L. Goodglick, M. Pold, S. Sharma, R. D. Ramirez, J. W. Shay, J. D. Minna, R. M. Strieter and S. M. Dubinett. 2005. IL-20, an anti-angiogenic cytokine that inhibits COX-2 expression. Biochem. Biophys. Res. Commun. 333, 470-475   DOI   ScienceOn
2 Nieminen, R., S. Leinonen, A. Lahti, K. Vuolteenaho, U. Jalonen, H. Kankaanranta, M. B. Goldring and E. Moilanen. 2005. Inhibitors of mitogen-activated protein kinases downregulate COX-2 expression in human chondrocytes. Mediators Inflamm. 5, 249-255
3 Sawaoka, H., S. Tsuji, M. Tsujii, E. S. Gunawan, Y. Sasaki, S. Kawano and M. Hori. 1999. Cyclooxygenase inhibitors suppress angiogenesis and reduce tumor growth in vivo. Lab. Invest. 79, 1469-1477
4 Chen, P., Y. Cai, Z. G. Yang, R. Zhou, G. S. Zhang, F. Domann and X. Fang. 2006. Involvement of PKC, p38 MAPK and AP-2 in IL-1$\beta$-induced expression of cyclooxygenase- 2 in human pulmonary epithelial cells. Respirology 11, 18-23   DOI   ScienceOn
5 Jin, D. Z., L. L. Yin, X. Q. Ji and X. Z. Zhu. 2006. Cryptotanshinone inhibits cyclooxygenase-2 enzyme activity but not its expression. Eur. J. Pharmacol. 549, 166-172   DOI   ScienceOn
6 Lee, E., M. K. Choi, Y. J. Lee, J. L. Ku, K. H. Kim, J. S. Choi and S. J. Lim. 2006. $\alpha$-tocopheryl succinate, in contrast to $\alpha$-tocopherol and $\alpha$-tocopheryl acetate, inhibits prostaglandin E2 production in human lung epithelial cells. Carcinogenesis 27, 2308-2315   DOI   ScienceOn
7 Kim, S. O., K. S. Chun, J. K. Kundu and Y. J. Surh. 2004. Inhibitory effects of [6]-gingerol on PMA-induced COX-2 expression and activation of NF- $\kappa B$ and p38 MAPK in mouse skin. Biofactors 21, 27-31   DOI   ScienceOn
8 Koki, A., N. K. Khan, B. M. Woerner, A. J. Dannenberg, L. Olson, K. Seibert, D. Edwards, M. Hardy, P. Isakson and J. L. Masferrer. 2002. Cyclooxygenase-2 in human pathological disease. Adv. Exp. Med. Biol. 507, 177-184   DOI
9 Brunelle, M., E. A. Sartin, L. G. Wolfe, J. Sirois and M. Dore. 2006. Cyclooxygenase-2 expression in normal and neoplastic canine mammary cell lines. Vet. Pathol. 43, 656-666   DOI   ScienceOn
10 Johnson, A. C. 1996. Activation of epidermal growth factor receptor gene transcription by phorbol 12-myristate 13-acetate is mediated by activator protein 2. J. Biol. Chem. 271, 3033-3038
11 Lee, J. O., M. H. Park, Y. H. Choi, Y. L. Ha and C. H. Ryu. 2007. New fermentation technique for complete digestion of soybean protein. J. Microbiol. Biotechn. 17, 1904-1907   과학기술학회마을
12 Ke, J., X. Long, Y. Liu, Y. F. Zhang, J. Li, W. Fang and Q. G. Meng. 2007. Role of NF-$\kappa B$ in TNF-$\alpha$-induced COX-2 expression in synovial fibroblasts from human TMJ. J. Dent. Res. 86, 363-367   DOI   ScienceOn
13 Brown, J. R. and R. N. DuBois. 2004. Cyclooxygenase as a target in lung cancer. Clin. Cancer Res. 10, 4266s-4269s   DOI   ScienceOn
14 Johnson, K. R., K. P. Becker, M. M. Facchinetti, Y. A. Hannun and L. M. Obeid. 2005. PKC-dependent activation of sphingosine kinase 1 and translocation to the plasma membrane. Extracellular release of sphingosine-1-phosphate induced by phorbol 12-myristate 13-acetate (PMA). J. Biol. Chem. 277, 35257-35262   DOI   ScienceOn
15 Soloff, M. S., D. L. Cook, Y. J. Jr, Jeng and G. D. Anderson. 2004. In situ analysis of interleukin-1-induced transcription of COX-2 and IL-8 in cultured human myometrial cells. Endocrinology 145, 1248-1254   DOI   ScienceOn
16 Seo, Y. J., M. S. Kwon, E. J. Shim, J. Y. Lee and H. W. Suh. 2006. The effects of phorbol 12-myristate 13-acetate, cholera toxin, prostaglandin E2 and norepinephrine on inducible nitric oxide synthase activation induced by lipopolysaccharide in C6 cells. Pharmacology 78, 178-184   DOI   ScienceOn
17 Thun, M. J., S. J. Henley and C. Patrono. 2002. Nonsteroidal anti-inflammatory drugs as anticancer agents: mechanistic, pharmacologic, and clinical issues. J. Natl. Cancer Inst. 94, 252-266   DOI   ScienceOn
18 Dempke, W., C. Rie, A. Grothey and H. J. Schmoll. 2001. Cyclooxygenase-2: a novel target for cancer chemotherapy? J. Cancer Res. Clin. Oncol. 127, 411-417   DOI   ScienceOn
19 Tong, M., Y. Ding and H. H. Tai. 2006. Reciprocal regulation of cyclooxygenase-2 and 15-hydroxyprostaglandin dehydrogenase expression in A549 human lung adenocarcinoma cells. Carcinogenesis 27, 2170-2179   DOI   ScienceOn
20 Balzary, R. W. and T. M. Cocks. 2006. Lipopolysaccharide induces epithelium- and prostaglandin E(2)-dependent relaxation of mouse isolated trachea through activation of cyclooxygenase (COX)-1 and COX-2. J. Pharmacol. Exp. Ther. 317, 806-812   DOI   ScienceOn
21 Yamamoto, Y. and R. B. Gaynor. 2001. Therapeutic potential of inhibition of the NF-$\kappa$B pathway in the treatment of inflammation and cancer. J. Clin. Invest. 107, 135-142   DOI   ScienceOn
22 Parekh, D. B., W. Ziegler and P. J. Parker. 2000. Multiple pathways control protein kinase C phosphorylation. EMBO J. 19, 496-503   DOI   ScienceOn
23 Giercksky, K. E. 2001. COX-2 inhibition and prevention of cancer. Best Pract. Res. Clin. Gastroenterol. 15, 821-833   DOI   ScienceOn
24 Herschman, H. R. 1996. Prostaglandin synthase 2. Biochim. Biophys. Acta. 1299, 125-40   DOI   ScienceOn
25 Jiang, Y. J., B. Lu, P. C. Choy and G. M. Hatch. 2003. Regulation of cytosolic phospholipase A2, cyclooxygenase- 1 and -2 expression by PMA, TNF$\alpha$, LPS and M-CSF in human monocytes and macrophages. Mol. Cell Biochem. 246, 31-38   DOI   ScienceOn
26 Reno, F. and M. Cannas. 2005. Effect of prostaglandin E2 on PMA-induced macrophage differentiation. Prostaglandins Other Lipid Mediat. 75, 13-24   DOI   ScienceOn
27 Basta, S., S. Knoetig, A. Summerfield and K. C. McCullough. 2001. Lipopolysaccharide and phorbol 12-myristate 13- acetate both impair monocyte differentiation, relating cellular function to virus susceptibility. Immunology 103, 488-497   DOI   ScienceOn
28 FitzGerald, G. A. 2003. COX-2 and beyond: Approaches to prostaglandin inhibition in human disease. Nat. Rev. Drug Discov. 2, 879-890   DOI   ScienceOn
29 Nishizuka, Y. 1995. Protein kinase C and lipid signaling for sustained cellular responses. FASEB J. 9, 484-496   DOI