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http://dx.doi.org/10.5352/JLS.2008.18.1.103

Purification and Properties of a Novel Extracellular Agarase from Marine Bacterium, Sphingomonas paucimobilis AS-1  

Jung, Il-Sun (Department of Food and Nutrition, College of Medical Life Science Silla University)
Kim, Yu-Jung (Department of Food and Nutrition, College of Medical Life Science Silla University)
Song, Hyo-Ju (Department of Food and Nutrition, College of Medical Life Science Silla University)
Gal, Sang-Wan (Department of Microbiological Engineering, Jinju National University)
Choi, Young-Ju (Department of Food and Nutrition, College of Medical Life Science Silla University)
Publication Information
Journal of Life Science / v.18, no.1, 2008 , pp. 103-108 More about this Journal
Abstract
An agar-degrading marine bacterium, strain AS-1 was isolated from the seawater. The strain AS-1 was identified as Sphingomonas paucimobilis (90% probability) by VITEK. The optimum medium for agarase activity of the isolated strain was determined to be marine medium, marine broth 2216 containing 0.1% agar as carbon source. An extracellular agarase was purified 104-fold from the culture supernatant by ammonium sulfate precipitation, ion exchange chromatography and gel filtration methods. The molecular weight of the purified enzyme was estimated to be 80 kDa by SDS-PAGE. The optimum pH and temperature for activity were 7.0 and $40^{\circ}C$, respectively. Antioxidative activity of the strain AS- was 72% in the supernatant cultured for 12 h. The culture supernatant of the strain AS-1 showed antibacterial activity against bacteria causing putrefaction and food poisoning such as Escherichia coli, Staphylococcus aureus and Proteus vulgaris. However, the cell growth of the lactic aicd forming strain, Lactobacillus plantarium was promoted by the treatment of 10% culture supernatant of an agar-degrading strain.
Keywords
Marine bacteria; agarase; Sphingomonas; purification; antioxidant;
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