Browse > Article
http://dx.doi.org/10.5352/JLS.2008.18.12.1700

Effect of Rheum undulatum Extract on Antioxidant Activity and Activity of Matrix Metalloproteinase-1 in Human Skin Fibroblasts  

Park, Sung-Min (R&D Center, CoSeedBioPharm Corporation)
Lee, Gye-Won (Department of Pharmaceutical Engineering, Konyang University)
Cho, Young-Ho (Department of Pharmaceutical Engineering, Konyang University)
Publication Information
Journal of Life Science / v.18, no.12, 2008 , pp. 1700-1704 More about this Journal
Abstract
Rheum undulatum L. has been commonly used as a cure for hematemesis, dropsy, and haematuria in the Oriental medicine for a long time. The main constituents of R. undulatum are chrysophanol and emodin, which are an antioxidative substance that has an anthraquinone structure. In the present study, to develop a new anti-aging agent, we examined the antioxidant activity and the inhibitory effect of the R. undulatum extract on the synthesis of MMP-1 in UVA-irradiated human dermal fibroblasts and MMP-1 activity. The R. undulatum extract was found to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and superoxide radicals in the xanthine/ xanthine oxidase system by a dose-dependent manner, respectively. UVA-induced MMP-1 expression was reduced about 79.5% by 1 ${\mu}g$/ml of the R. undulatum extract and also inhibited MMP-1 activity in a dose-dependent manner. In conclusion, it was observed that the R. undulatum extract has the antioxidant activity, regulation of UVA-induced MMP-1 production, and inhibition of MMP-1 activity. Therefore, these results suggest that the R. undulatum extract can be developed as a new anti-aging component of cosmetics.
Keywords
Rheum undulatum L.; emodin; antioxidant; MMP-1 inhibition;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Hatano, T. 1995. Constituents of natural medicines with scavenging effects on active oxygen species-tannins and related polyphenols. Natural Medicines 49, 357-363.
2 Jung, H. A., H. Y. Chung, T. Yokozawa, S. K. Kim and J. S. Choi. 2004. Alaternin and emodin with hydroxyl radical inhibitory and/or scavenging activities and hepatoprotective activity on tacrine-induced cytotoxicity in HepG2 cells. Arch. Pharm. Res. 27, 947-953.   DOI   ScienceOn
3 Kitahara, A., U. Matsumoto, H. Ueda and R. Ueoka. 1992. A remarkable antioxidation effect of natural phenol derivatives on the autoxidation of $\gamma$-irradiated methyl linolate. Chem. Pharm. Bull. 40, 2208-2209.   DOI
4 Kondo, S. 2000. The roles of cytokines in photoaging. J. Dermatol. Sci. 23, S30-S36.   DOI   ScienceOn
5 Kuppusamy, P. and J. L. Zweier. 1989. Characterization of free radical generation by xanthine oxidase. J. Biol. Chem. 264, 9880-9884.
6 Oh, S. J., N. I. Beak and H. Y. Kim. 2001. Piceatannol, Antioxidant compound isolated from the root of Rheum undulatum L. J. Korean Soc. Agric. Chem. Biotechnol. 44, 208-210.   과학기술학회마을
7 Mosmann, T. 1983. Rapid colorimetric assay for the cellular growth and survival: application to proliferation and cytotoxic assay. J. Immunol. Methods 65, 55-59.   DOI   ScienceOn
8 Lavker, R. M. and A. M. Kligman. 1988. Chronic heliodermatitis: a morphologic evaluation of chronic actinic dermal damage with emphasis on the role of mast cells. J. Invest. Dermatol. 90, 325-330.   DOI   ScienceOn
9 Masaki, H., S. Sakaki, T. Atsumi and H. Sakurai. 1995. Active oxygen scavenging activity of plant extracts. Biol. Pharm. Bull. 18, 162-166.   DOI   ScienceOn
10 Cho, Y. H., G. S. Sim, J. H. Kim, S. M. Park, B. C. Lee, H. B. Pyo, Y. P. Yun and H. D. Park. 2004. Effect of Melothria heterophylla extract on expression of matrix metalloproteinase- 1 in human skin fibroblasts. Yakhak Hoeji. 48, 358-363.   과학기술학회마을
11 Chung, J. H., S. W. Kang, J. Varani, J. Lin, G. J. Fisher and J. J. Voorhees. 2000. Decreased extracellular-signal regulated kinase and increased stress-activated MAP kinase activities in aged human skin in vivo. J. Invest. Dermatol. 115, 177-182.   DOI   ScienceOn
12 Furuno, K., T. Akasako and N. Sugihara. 2002. The contribution of the pyrogallol moiety to the superoxide radical scavenging activity of flavonoids. Biol. Pharm. Bull. 25, 19-23.   DOI   ScienceOn
13 Cadenas, E. 1989. Biochemistry of oxygen toxicity. Annu. Rev. Biochem. 58, 79-110.   DOI   ScienceOn
14 Brenneisen, P., H. Sies and K. Scharffetter-Kochanek. 2002. Ultraviolet-B irradiation and matrix metalloproteinases: from induction via signaling to initial events. Ann. N.Y. Acad. Sci. 973, 31-43.   DOI   ScienceOn
15 Blois, M. S. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181, 1199-1200.   DOI   ScienceOn
16 Wlaschek, M., K. Brviba, G. P. Stricklin, H. Sies and K. Scharffetter-Kochanek. 1995. Singlet oxygen may mediate the ultraviolet A induced synthesis of interstitial collagenase. J. Invest. Dermatol. 104, 194-198.   DOI   ScienceOn
17 Wang, Y., A. R. Johnson, Q. Z. Ye and R. D. Dyer. 1999. Catalytic activities and substrate specificity of the human membrane type 4 matrix metalloproteinase catalytic domain. J. Biol. Chem. 274, 33043-33049.   DOI
18 Park, J. H. 2002. The Encyclopedia of Chinese Crude Drugs. Shinilbooks Publications, Seoul, pp. 180-184.
19 Shah, C. S., J. S. Qadry and J. G. Bhatt. 1972. Qualitative and quantitative evaluation of anthraquinone derivatives in Indian thubarb. Plant Med. 22, 103-108.   DOI
20 Davies, K. J. 1987. Protein damage and degradation by oxygen radicals. J. Biol. Chem. 262, 9914-9920.
21 Kochanek, S. K., M. Wlaschek, K. Briviba and H. Sies. 1993. Singlet oxygen induces collagenase expression in human skin fibroblast. FEBS Lett. 331, 304-306.   DOI   ScienceOn