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http://dx.doi.org/10.5352/JLS.2008.18.11.1485

Prostaglandin A2-induced Apoptosis is Not Inhibited by Heme Oygenase-1 in U2OS Cells  

Ko, Kyoung-Won (Department of Biochemistry, College of Medicine, The Catholic University of Korea)
Lee, Sun-Young (Department of Biochemistry, College of Medicine, The Catholic University of Korea)
Ahn, Ji-Hyun (Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Chung-Ang University)
Kim, Jae-Taek (Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Chung-Ang University)
Kim, In-Kyung (Department of Biochemistry, College of Medicine, The Catholic University of Korea)
Kim, Ho-Shik (Department of Biochemistry, College of Medicine, The Catholic University of Korea)
Publication Information
Journal of Life Science / v.18, no.11, 2008 , pp. 1485-1492 More about this Journal
Abstract
Prostaglandin $A_2$ ($PGA_2$), one of cyclopentenone PGs, induced both apoptosis and heme oxygenase (HO)-1 expression in U2OS cells. $PGA_2$-induced apoptosis was not perturbed by either over-expression or knock-down of HO-1, whereas $H_2O_2$-induced cell death was inversely modulated by the expression level of HO-1. In addition, N-acetyl-L-cysteine (NAC), a thiol antioxidant, blocked both apoptosis and HO-1 expression induced by $PGA_2$. But, non-thiol antioxidants like butylated hydorxyanisole (BHA) and ascorbic acid did not block either apoptosis or HO-1-induction. Taken together, these results suggest that $PGA_2$ induces both apoptosis and HO-1 expression, which are critically related to the thiol- reactivity of $PGA_2$, but not oxidative stress, and HO-1 expression may be independent or functionally located downstream of apoptosis by $PGA_2$ without contribution to apoptosis progression.
Keywords
Prostaglandin; cyclopentenone; apoptosis; heme oxygenase-1; oxidative stress;
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1 Ahn, S. G., S. Y. Jeong, H. Rhim H and I. K. Kim. 1998. The role of c-Myc and heat shock protein 70 in human hepatocarcinoma Hep3B cells during apoptosis induced by prostaglandin A2/Delta12-prostaglandin J2. Biochim. Biophys. Acta 1448, 115-125.   DOI   ScienceOn
2 Kim, H. S., H. Rhim, S. W. Jeong, J. W. Kim and I. K. Kim. 2002. Induction of apoptosis dependent on caspase activities and growth arrest in HL-60 cells by PGA2. Prostaglandins Other Lipid Mediat. 70, 169-183.   DOI   ScienceOn
3 Choi, A. M., J. Fargnoli, S. G. Carlson and N. J. Holbrook. 1992. Cell growth inhibition by prostaglandin A2 results in elevated expression of gadd153 mRNA. Exp. Cell. Res. 199, 85-89.   DOI   ScienceOn
4 Chen, Y. C., S. C. Shen and S. H. Tsai. 2005. Prostaglandin D(2) and J(2) induce apoptosis in human leukemia cells via activation of the caspase 3 cascade and production of reactive oxygen species. Biochim. Biophys. Acta 1743, 291-304.   DOI   ScienceOn
5 Sanchez-Gomez, F. J., E. Cernuda-Morollon, K. Stamatakis and D. Perez-Sala. 2004. Protein thiol modification by 15-deoxy-Delta12,14-prostaglandin J2 addition in mesangial cells: role in the inhibition of pro-inflammatory genes. Mol. Pharmacol. 66, 1349-1358.   DOI   ScienceOn
6 Rossi, A., P. Kapahi, G. Natoli, T. Takahashi, Y. Chen, M. Karin and M. G. Santoro. 2000. Anti-inflammatory cyclopentenone prostaglandins are direct inhibitors of IkappaB kinase. Nature 403, 103-108.   DOI   ScienceOn
7 Zhuang, H., S. Pin, X. Li and S. Dore. 2003. Regulation of heme oxygenase expression by cyclopentenone prostaglandins. Exp. Biol. Med. (Maywood) 228, 499-505.   DOI
8 Straus, D. S. and C. K. Glass. 2001. Cyclopentenone prostaglandins: new insights on biological activities and cellular targets. Med. Res. Rev. 21, 185-210.   DOI   ScienceOn
9 Ryter, S. W., L. E. Otterbein, D. Morse and A. M. Choi. 2002. Heme oxygenase/carbon monoxide signaling pathways: regulation and functional significance. Mol. Cell. Biochem. 234-235, 249-263.   DOI   ScienceOn
10 Radhakrishnan, N., M. Bhaskaran and P. C. Singhal. 2005. Hepatocyte growth factor modulates H2O2-induced mesangial cell apoptosis through induction of heme oxygenase-1. Nephron Physiol. 101, 92-98.   DOI   ScienceOn
11 Otterbein, L. E., M. P. Soares, K. Yamashita and F. H. Bach. 2003. Heme oxygenase-1: unleashing the protective properties of heme. Trends Immunol. 24, 449-455.   DOI   ScienceOn
12 Narumiya, S., K. Ohno, M. Fukushima and M. Fujiwara. 1987. Site and mechanism of growth inhibition by prostaglandins. III. Distribution and binding of prostaglandin A2 and delta 12-prostaglandin J2 in nuclei. J. Pharmacol. Exp. Ther. 242, 306-311.
13 Narumiya, S., K. Ohno, M. Fujiwara and M. Fukushima. 1986. Site and mechanism of growth inhibition by prostaglandins. II. Temperature-dependent transfer of a cyclopentenone prostaglandin to nuclei. J. Pharmacol. Exp. Ther. 239, 506-511.
14 Parker, J. and H. Ankel. 1992 Formation of a prostaglandin A2-glutathione conjugate in L1210 mouse leukemia cells. Biochem. Pharmacol. 43, 1053-1060.   DOI   ScienceOn
15 Ohno, K., M. Hirata, S. Narumiya and M. Fukushima. 1992. Effect of glutathione content on cellular uptake and growth inhibitory activity of prostaglandin A2 in L-1210 cells. Eicosanoids 5, 81-85.
16 Kim, H. S., J. H. Lee and I. K. Kim. 1996. Intracellular glutathione level modulates the induction of apoptosis by delta 12-prostaglandin J2. Prostaglandins 51, 413-425.   DOI   ScienceOn
17 Li Volti, G., V. Sorrenti, P. Murabito, F. Galvano, M. Veroux, A. Gullo, R. Acquaviva, A. Stacchiotti, F. Bonomini, L. Vanella and C. Di Giacomo. 2007. Pharmacological induction of heme oxygenase-1 inhibits iNOS and oxidative stress in renal ischemia-reperfusion injury. Transplant. Proc. 39, 2986-2991.   DOI   ScienceOn
18 Lee, P. J., J. Alam, G. W. Wiegand and A. M. Choi. 1996. Overexpression of heme oxygenase-1 in human pulmonary epithelial cells results in cell growth arrest and increased resistance to hyperoxia. Proc. Natl. Acad. Sci. USA 93, 10393-10398.   DOI   ScienceOn
19 Livak, K. J. and T. D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-$\bigtriangleup$$\bigtriangleup$C(T)) method. Methods 25, 402-408.   DOI   ScienceOn
20 Nencioni, A., K. Lauber, F. Grunebach, L. Van Parijs, C. Denzlinger, S. Wesselborg and P. Brossart. 2003. Cyclopentenone prostaglandins induce lymphocyte apoptosis by activating the mitochondrial apoptosis pathway independent of external death receptor signaling. J. Immunol. 171, 5148-5156.   DOI
21 Kim, I. K., J. H. Lee, H. W. Sohn, H. S. Kim and S. H. Kim SH. 1993. Prostaglandin A2 and delta 12-prostaglandin J2 induce apoptosis in L1210 cells. FEBS Lett. 321, 209-214.   DOI   ScienceOn
22 Kobayashi, M., H. Ono, K. Mihara, H. Tauchi, K. Komatsu, T. Shibata, H. Shimizu, K. Uchida and K. Yamamoto. 2006. ATM activation by a sulfhydryl-reactive inflammatory cyclopentenone prostaglandin. Genes Cells 11, 779-789.   DOI   ScienceOn
23 Hubich, A. I. and M. V. Sholukh. 2006. Biochemistry of prostaglandins A. Biochemistry (Mosc) 71, 229-238.   DOI
24 Lee, J. S. and Y. J. Surh. 2005. Nrf2 as a novel molecular target for chemoprevention. Cancer Lett. 224, 171-184.   DOI   ScienceOn
25 Lee, J. H., H. S. Kim, S. Y. Jeong and I. K. Kim. 1995. Induction of p53 and apoptosis by delta 12-PGJ2 in human hepatocarcinoma SK-HEP-1 cells. FEBS Lett. 368, 348-352.   DOI   ScienceOn
26 Holbrook, N. J., S. G. Carlson, A. M. Choi and J. Fargnoli. 1992. Induction of HSP70 gene expression by the antiproliferative prostaglandin PGA2: a growth-dependent response mediated by activation of heat shock transcription factor. Mol. Cell. Biol. 12, 1528-1534.   DOI
27 Gorospe, M., Y. Liu, Q. Xu, F. J. Chrest and N. J. Holbrook. 1996. Inhibition of G1 cyclin-dependent kinase activity during growth arrest of human breast carcinoma cells by prostaglandin A2. Mol. Cell. Biol. 16, 762-770.   DOI
28 Kim, H. S., J. C. Shim, J. Y. Choi, H. Rhim and I. K. Kim. 2005. Prostaglandin A2 induces caspase-independent apoptosis in hepatocellular carcinoma cells. Korean J. Hepatol. 11, 72-79.
29 Poss, K. D. and S. Tonegawa. 1997. Reduced stress defense in heme oxygenase 1-deficient cells. Proc. Natl. Acad. Sci. U S A 94, 10925-10930.   DOI
30 Kim, H. R., S. Kim, E. J. Kim, J. H. Park, S. H. Yang, E. T. Jeong, C. Park, M. J. Youn, H. S. So and R. Park. 2008. Suppression of Nrf2-driven heme oxygenase-1 enhances the chemosensitivity of lung cancer A549 cells toward cisplatin. Lung Cancer 60, 47-56.   DOI   ScienceOn
31 Alvarez-Maqueda, M., R. El Bekay, G. Alba, J. Monteseirln, P. Chacon, A. Vega, J. Martln-Nieto, F. J. Bedoya, E. Pintado and F. Sobrino. 2004. 15-deoxy-delta 12,14-prostaglandin J2 induces heme oxygenase-1 gene expression in a reactive oxygen species-dependent manner in human lymphocytes. J. Biol. Chem. 279, 21929-21937.   DOI   ScienceOn
32 Alam, J. and J. L. Cook. 2003. Transcriptional regulation of the heme oxygenase-1 gene via the stress response element pathway. Curr. Pharm. Des. 9, 2499-2511.   DOI   ScienceOn