Browse > Article
http://dx.doi.org/10.5352/JLS.2008.18.10.1435

Anticancer Activity of Ethanol Extract from Peel of Citrus junos and Poncirus trifoliata on MCF-7 Breast Cancer Cells  

Kim, Ji-Eun (Department of Biotechnology, Dong-A University)
Park, Joon-Hee (Department of Molecular Biology, Dong-Eui University)
Kang, Byoung-Won (BK21 Center for Silver-Bio industrialization Project, Dong-A University)
Seo, Min-Jeong (Department of Biotechnology, Dong-A University)
Choi, Yung-Hyun (Department of Biochemistry, College of Oriental Medicine, Dong-Eui University)
Lim, Hak-Seob (Department Bioinstitute, MILLENNIUM PROMISE CO., LTD.)
Seo, Kwon-Il (Department of Food and Nutrition, Sunchon National University)
Kim, Jeong-In (School of Food and Life Science, Inje University)
Joo, Woo-Hong (Department of Biology, Changwon National University)
Lee, Bok-Kyu (Department of Molecular Biology, Dong-Eui University)
Jeong, Yong-Kee (Department of Biotechnology, Dong-A University)
Publication Information
Journal of Life Science / v.18, no.10, 2008 , pp. 1435-1441 More about this Journal
Abstract
In this study, anti-cancer activities of peel of Citrus junos (CJP) and Poncirus trifoliata (PTP) on MCF-7 breast cancer cells, and anti-proliferative effects of cancer cells induced by environmental hormones were investigated. The ethanol extracts of CJP and PTP inhibited cancer cell growth and induced apoptosis at the concentration over 300 mg/ml treatment for 72 hr. Morphological change of MCF-7 breast cancer cells were observed treated with the CJP and PTP of 500 mg/ml concentration for 72 hr, and apoptosis was induced by activation of caspase-3. The proliferation of MCF-7 breast cancer cells was decreased in a dose-dependent manner treated with various concentration of CJP and PTP, when compared with the control at 300 mg/ml, the proliferation of the MCF-7 breast cancer cells of both extracts was decreased over 70% and 80%, respectively.
Keywords
Poncirus trifoliata; Citrus junos; MCF-7 breast cancer cell; anti-cancer activity; antiproliferative effect;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
연도 인용수 순위
1 Kim, H. W., S. J. Cho, B. Y. Kim and S. I. Cho 2007. Anti-oxidant effects of Anemarrhenae Rhizoma in three different lineages. Kor. J. Orient. Int. Med. 28, 608-614.   과학기술학회마을
2 Kang, S. K., M. J. Jang and Y. D. Kim. 2006. A study on the flavor constituents of the Citron (Citrus junos). Kor. J. Food Preserv. 13, 204-210.   과학기술학회마을
3 Kim, T. J., J. Y. No and J. S. Ko. 1989. The separation and determination of flavonoid glycosides from Poncirus trifoliata rafia and Citrus aurantium. L. J. Kor. Soc. Ana. Sci. 2, 302-307.
4 Hwang, Y. J., H. K. Nam, M. J. Chang, G. W. Noh and S. H. Kim. 2003. Effect of Lentinus edodes and Pleurotus eryngii. Extracts on proliferation and apoptosis in human colon cancer cell lines. J. Kor Soc. Food Sci. Nutr. 32, 217-222.   과학기술학회마을   DOI   ScienceOn
5 Aobchey, P., S. Sinchaikul, S. Phutrakul and S. T. Chen. 2007. Simple purification of indirubin from Indigofera tinctoria Linn. and inhibitory effect on MCF-7 human breast cancer cells. Chiang Mai Journal of Science 34, 329-337.
6 Park, K. D., B. S. Han and K. H. Rhim. 2006. Modifying effect of bisphenol A on the preneoplastic and neoplastic lesion if rat liver treated with diethylnitrosamine infusion. Kor. J. Env. Hlth. 32, 60-66.   과학기술학회마을
7 Chung, H. S., J. B. Lee, J. H. Seong and J. U. Choi. 2004. Chemical components in peel and flesh of trifoliata oranges (Poncirus trifoliata). Kor. J. Food Pre. 11, 342-346.   과학기술학회마을
8 Jayaprakasha, G. K., K. Mandadi, K. Poulose, M. Shibu, Y. Jadegoud, G. Gowda, G. A. Nagana, G. Patil and S. Bhimanagouda. 2007. Inhibition of colon cancer cell growth and antioxidant activity of bioactive compounds from Poncirus trifoliata (L.) Raf. Bioorganic & Medicinal Chemistry 15, 4923-4932.   DOI   ScienceOn
9 Kang, S. C., C. M. Lee, E. S. Choung, J. P. Bak, J. J. Bae, H. S. Yoo, J. H. Kwak and O. P. Zee. 2008. Anti-proliferative effects of estrogen receptor-modulating compounds isolated from Rheum palmatum. Archives of Pharmacal Research 31, 722-726.   과학기술학회마을   DOI   ScienceOn
10 Skehan, P. R., D. Stoveng, A. Scudiero, J. Monks, D. McMahon, J. T. Vistica, H. Warren, S. Bokesch, M. Kenney and R. Boyd. 1990. New colorimetric cytotoxicity assay for anticancer drug screening. T. Nat. Cancer Inst. 82, 1107-1112.   DOI
11 Soung, M. G., S. M. Joo, A. R. Song and N. D. Sung. 2007. QSAR on the inhibition activity of flavopiridol analogues against breast cancer MCF-7. J. Korean Soc. Appl. Biol. Chem. 50, 147-153   과학기술학회마을
12 Choi, Y. H., J. H. Baek, M. A. Yoo, H. Y. Chung and K. W. Kim. 2000. Induction of apoptosis by ursolic acid through activation of caspases and down-regulation of c-IAPs in human prostate epithelial cells. Int. J. Oncol. 17, 971-976.
13 Choi, Y. H., H. S. Eo, Y. G. Jin, Y. H. Yoo and N. Kim. 2003. Apoptosis and modulation of cell control by synthetic derivatives of ursodeoxycholic acid and chenodeoxycholic acid in human prostate cancer cells. Cancer Letters 199, 157-167.   DOI   ScienceOn
14 Chen, W. F., M. H. Huang, C. H. Tzang, M. Yang and M. S. Wong. 2003. Inhibitory actions of genistein in human breast cancer (MCF-7) cells. Biochimica et Biophysica Acta. Molecular Basis of Disease 1638, 187-196.   DOI   ScienceOn
15 Dong, S., A. Inoue, Y. Zyu, M. Tanji and R. Kiyama. 2007. Activation of rapid signaling pathways and the subsequent transcriptional regulation for the proliferation of breast cancer MCF-7 cells by the treatment with an extract of Glycyrrhiza glabra root. Food and Chemical Toxicology 45, 2470-2478.   DOI   ScienceOn
16 Lee, Y. G. and I. H. Cha. 2001. Antibacterial activity of Poncirus trifoliata juice against pathogenic bacteria. Kor. J. Life Sci. 11, 534-560.   과학기술학회마을
17 Murakami, H. and T. Sugahara. 1994. Animal cell technology, pp. 215-233, Corona Publishing Co. Japan.
18 Nakagawa, Y. and T. Suzuki. 2001. Metabolism for bisphenol A in isolated rat hepatocytes and estrogenic activity of a hydroxylated metabolite in MCF-7 human breast cancer cell. Xenobiotica 31, 113-123.   DOI   ScienceOn
19 Steller, H. 1995. Mechanisms and genes of cellular suicide. Science 267, 1445-1449.   DOI   ScienceOn
20 Yi, J. M., M. S. Kim, H. N. Koo, B. K. Song, H. Y. Yoo and H. M. Kim. 2004. Poncirus trifoliata fruit induces apoptosis in human promyelocytic leukemia cells. Clinica Chimica Acta. 340, 179-185.   DOI   ScienceOn
21 Yoo, K. M. and I. K. Hwang. 2004. In vitro Effect of Yuza (Citrus junos SIEB ex TANAKA) extracts on proliferation of human prostate cancer cells and antioxidant activity. Kor. J. Food Sci. Technol. 36, 339-344.   과학기술학회마을
22 Kawaii, S., Y. Tomono, E. Katase, K. Ogawa and M. Yano. 1999. Quantitation of flavonoid constituents in citrus fruits. J. Agric. Food Chem. 47, 3565-3571.   DOI   ScienceOn
23 Han, D. H., Y. Miyazaki and H. Tachibana. 2000. Optimization of estrogenic activity detection method using human breast cancer MCF-7 cells. J. Fac. Agr., Kyushm Univ. 44, 339-348.
24 Johnson, M. K. and G. Loo. 2000. Effects of epigallocatechin gallate and quercetin on oxidative damage to cellular DNA. Mutat. RES. 459, 211-218.   DOI   ScienceOn