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http://dx.doi.org/10.5352/JLS.2007.17.8.1157

Cloning and Expression Analysis of a Grape asr gene, VlASR Containing a Promoter Region.  

Kihl, Joon-Yeong (Department of Molecular Biology and Institute of Nanosensor and Biotechnology, Dankook University)
Pyee, Jae-Ho (Department of Molecular Biology and Institute of Nanosensor and Biotechnology, Dankook University)
Publication Information
Journal of Life Science / v.17, no.8, 2007 , pp. 1157-1165 More about this Journal
Abstract
VvMSA, a grapevine ASR which is highly inducible by sugar and abscisic acid signals was previously shown to be a transcription factor for a hexose transporter gene VvHT1. We isolated a cDNA clone, VlASR which is regulated temporally during the grape berry development by ACP RT-PCR (annealing control primer reverse transcriptase-polymerase chain reaction) and it proved identical to VvMSA. RT-PCR and real-time PCR analyses revealed that the VlASR gene was expressed in berries at fruit set and that its expression increased as berries aged but decreased at the late ripening stage. In order to understand the regulatory mechanism of the asr gene, a genomic fragment was cloned from grapevine. The genomic DNA was 1375 bp long and a sugar box (sucrose box 3 and sucrose responsive element 1) was identified in the 611 bp upstream region of the open reading frame. Analysis of the VlASR promoter::reporter gene fusion demonstrated that this promoter was expressed in transgenic Arabidopsis even without sucrose treatment. This result suggests that the ASR/VvHT1-mediated sugar/ABA signaling, previously reported in grapevine, may not function in Arabidopsis which has no ASR homologue.
Keywords
Grape asr gene; promoter; fruit ripening; sugar box;
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