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http://dx.doi.org/10.5352/JLS.2007.17.8.1129

Bombyx mori Protein Disulfide Isomerase (bPDI) Protects Sf9 Cells from Endoplasmic Reticulum (ER) Stress  

Goo, Tae-Won (Department of Agricultural biology, the National Institute of Agriculture Science and Technology)
Yun, Eun-Young (Department of Agricultural biology, the National Institute of Agriculture Science and Technology)
Kim, Sung-Wan (Department of Agricultural biology, the National Institute of Agriculture Science and Technology)
Choi, Kwang-Ho (Department of Agricultural biology, the National Institute of Agriculture Science and Technology)
Kang, Seok-Woo (Department of Agricultural biology, the National Institute of Agriculture Science and Technology)
Kwon, Ki-Sang (Department of Anatomy, College of Medicine, Chungnam National University)
Kwon, O-Yu (Department of Anatomy, College of Medicine, Chungnam National University)
Publication Information
Journal of Life Science / v.17, no.8, 2007 , pp. 1129-1134 More about this Journal
Abstract
In the previous our study, a cDNA that encodes protein disulfide isomerase from Bombyx mori (bPDI)was isolated and characterized. bPDI has an open reading frame of 494 amino acids contained two PDI-typical thioredoxin active site of WCGHCK and ER (endoplasmic reticulum) retention signal of the KDEL motif at its C-terminal. Recent studies have demonstrated that misfolded proteins are accumulated in many diseases including Alzheimer’s, goiter, emphysema, and prion infections. bPDI was over-expressed or knock-downed in Sf9 cells to study the relationship between bPDI expression and protections against protein misfolding. bPDI gene was cloned in insect expression vector pIZT/V5-His for over-expression and bPDI double-stranded RNA (dsRNA) was generated for knock-down. Over-expression of bPDI significantly improved survival rate, but bPDI dsRNA transfection significantly reduced survival rate after 48 hours exposure. In mock-transfected or wild-type cells had no significant effect. The results support the view that bPDI is one of the important intracellular components for cell protect mechanism, especially, against ER stress such as protein misfolding.
Keywords
Bombyx mori; protein disulfide isomerase; endoplamic reticulum; misfolding;
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