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http://dx.doi.org/10.5352/JLS.2007.17.8.1090

GATA-3 is a Key Factor for Th1/Th2 Balance Regulation by Myristicin in a Murine Model of Asthma  

Lee, Kyu (Department of Microbiology and Immunology, Pusan National University College of Medicine, National Research Laboratory Of Dendritic Cell Differentiation & Regulation, Pusan National University College of Medicine)
Lee, Chang-Min (Department of Microbiology and Immunology, Pusan National University College of Medicine, National Research Laboratory Of Dendritic Cell Differentiation & Regulation, Pusan National University College of Medicine)
Jung, In-Duk (Department of Microbiology and Immunology, Pusan National University College of Medicine, National Research Laboratory Of Dendritic Cell Differentiation & Regulation, Pusan National University College of Medicine)
Jeong, Young-Il (National Research Laboratory Of Dendritic Cell Differentiation & Regulation, Pusan National University College of Medicine, Department of Microbiology, Pusan National University College of Nature Science)
Chun, Sung-Hak (National Research Laboratory Of Dendritic Cell Differentiation & Regulation, Pusan National University College of Medicine)
Park, Hee-Ju (Department of Pediatrics, Pusan National University College of Medicine)
Choi, Il-Whan (Department of Microbiology, Inje University College of Medicine)
Ahn, Soon-Cheol (Department of Microbiology and Immunology, Pusan National University College of Medicine)
Shin, Yong-Kyoo (Department of Pharmacology, Chungang University College of Medicine)
Lee, Sang-Yull (Department of Biochemistry, Pusan National University college of Medicine)
Yeom, Seok-Ran (Department of Emergency Medicine, Pusan National University college of Medicine)
Kim, Jong-Suk (Department of Biochemistry, Chonbuk National University Medical School)
Park, Yeong-Min (Department of Microbiology and Immunology, Pusan National University College of Medicine, National Research Laboratory Of Dendritic Cell Differentiation & Regulation, Pusan National University College of Medicine)
Publication Information
Journal of Life Science / v.17, no.8, 2007 , pp. 1090-1099 More about this Journal
Abstract
Myristicin, l-allyl-3,4-methylenedioxy-5-methoxybenzene, was one of the major essential oils of nutmeg. However, its anti-allergic effect in the Th1/Th2 immune response was poorly understood. Recently, it was shown that T-bet and GATA-3 was master Th1 and Th2 regulatory transcription factors. In this study, we have attempted to determine whether myristicin regulates Th1/Th2 cytokine production, T-bet and GATA-3 gene expression in ovalbumin (OVA)-induced asthma model mice. Myristicin reduced levels of IL-4, Th2 cytokine production in OVA-sensitized and challenged mice. In the other side, it increased $IFN-{\gamma}$, Th1 cytokine production in myristicin administrated mice. We also examined to ascertain whether myristicin could influence eosinophil peroxidase (EPO) activity. After being sensitized and challenged with ovalbumin (OVA) showed typical asthmatic reactions. These reactions included an increase in the number of eosinophils in bronchoalveolar lavage fluid, an increase in inflammatory cell infiltration into the lung tissue around blood vessels and airways, and the development of airway hyper-responsiveness (AHR). The administration of myristicin before the last airway OVA challenge resulted in a significant inhibition of all asthmatic reactions. Accordingly, these findings provide new insight into the immunopharmacological role of myristicin in terms of its effects in a murine model of asthma.
Keywords
Asthma; ovalbumin; myristicin; MMP-9; GATA-3; T-bet; IL-4; IL-5; airway hyper re-sponsiveness;
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