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http://dx.doi.org/10.5352/JLS.2007.17.8.1039

Chemotherapeutic Drug Resistant Cancer Stem-like Cells of Glioma  

Kang, Mi-Kyung (Department of Physiology, College of Medicine, Pusan National University)
Kang, Soo-Kyung (Department of Physiology, College of Medicine, Pusan National University)
Publication Information
Journal of Life Science / v.17, no.8, 2007 , pp. 1039-1045 More about this Journal
Abstract
Glioblastoma multiforme (GBM) is the most frequently occurring brain cancer. Although the existence of cancer stem cells (CSCs) in GBM has been established, there is little evidence to explain the link between CSCs and chemoresistance. In this study, we investigated that only a few cells of A172 and established GBM2 survived after 1,3-bis(2chloroethyl)-1-nitrosourea (BiCNU) exposures and these sur-vived cells resist the subsequent BiCNU treatment. In addition, these BiCNU-resistant small pop-ulations derived from GBM cells increased the phosphorylations of Erk and Akt and highly expressed CD133 stem cell surface marker. Furthermore, we observed that the BiCNU-resistant cancer cells de-rived from GBM have grown tumors when transplanted into severe combined immuno-deficient (SCID) mouse brain. These results demonstrate that BiCNU-resistant subpopulation cells derived from GBM have cancer stem-like cell properties. Therefore, it may provide provide further evidence that CSCs in GBM have chemotherapeutic drug resistance.
Keywords
Glioblastoma multiforme; cancer stem cells; chemoresistance; 1,3-bis(2-chloroethyl)-1-nitrosourea; tumor;
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