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http://dx.doi.org/10.5352/JLS.2007.17.5.687

Effects of glucose on metabolism and Insulin-like growth factor binding-3 expression in human fibroblasts.  

Ryu, Hye-Young (Faculty of Food Science and Biotechnology, Pukyung National University)
Hwang, Hye-Jung (Faculty of Food Science and Biotechnology, Pukyung National University)
Kim, In-Hye (Faculty of Food Science and Biotechnology, Pukyung National University)
Ryu, Hong-Soo (Faculty of Food Science and Biotechnology, Pukyung National University)
Nam, Taek-Jeong (Faculty of Food Science and Biotechnology, Pukyung National University)
Publication Information
Journal of Life Science / v.17, no.5, 2007 , pp. 687-693 More about this Journal
Abstract
Insulin-like growth factor-I(IGF-I) has significant insulin-like anabolic effects which include the stimulation of glucose and amino acid uptake, as well as protein and glycogen synthesis. IGFs exist in serum and other biological fluids as complexes bound to a family of structurally related insulin-like growth factor binding proteins(IGFBPs). Six human IGFBPs can modulate the effects of IGFs on target tissues by several mechanisms, including altering the serum's half-life and the transcapillary transport of IGFs, as well as changing the availability of IGFs to specific cell surface receptors. Human fibroblasts secrete IGFBPs that can modify IGF-I action. Previous to our study using either Northern blotting, and Western blotting have shown that fibroblasts express mRNA IGFBP-3, -4, and -5, and synthesize these proteins. In addition, fibroblast cell lysates revealed that the IGFBP-3 was most abundant. For these reasons, we undertook to gain further insight into the effects of high and low glucose incubation condition on metabolism and IGFBP-3 expression. In results of metabolites and IGFBP-3 expression in GM10 cells cultivated with various glucose concentration, the consumption of glucose and accumulation of triglyceride were increased in condition of high glucose, and total protein level was decreased. in the course of time. After 5 days incubation, levels of free amino acid in medium containing glucose of high concentration glucose were higher than in conditions of low glucose. Although the levels of IGFBP-3 protein and mRNA levels were increased in low glucose, and IGFBP-3 was not affected by any pretense. Taken together, we suggest that the study of growth factors, like IGFs, might be a possible model of diabetes militus in cell, although the results in cell models were not in accord with in vivo.
Keywords
IGFs; IGFBP-3; glucose; diabetes;
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