Browse > Article
http://dx.doi.org/10.5352/JLS.2007.17.4.587

Current Progress in Generation of Genetically Modified Mice  

Song, Ki-Duk (NICHD/National Institutes of Health)
Cho, Byung-Wook (College of Natural Resources & Life Science/PNU-IGB Joint Research Center, National University)
Publication Information
Journal of Life Science / v.17, no.4, 2007 , pp. 587-592 More about this Journal
Abstract
Manipulation of the mouse genome by activating or inactivating the gene has contributed to the understanding of the function of the gene in the subset of cells during embryonic development or postnatal period of life. Most of all, gene targeting, which largely depends on the availability of mouse embryonic stem (ES) cells, is the milestone of development of animal models for human disease. Recombinase-mediated genome modification (Cre-LoxP and Flp-Frt etc) and the ligand-dependent regulation system, more accurate and elaborate manipulation tools, have been successfully developed and applied to dissect the mechanisms governing complex biological processes and to understand the role of protein in temporal-and spatial aspects of development. As technologies concerning refined manipulation of mouse genome are developed, they are expected to open new opportunities to better understand the diverse in vivo functions of genes.
Keywords
Embryonic stem cell; gene targeting; genetically modified mice; site-specific recombinase;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Brocard, J., X. Warot, O. Wendling, N. Messaddeq, J. L. Vonesch, P. Chambon and D. Metzger. 1997. Spatio-temporally controlled site-specific somatic mutagenesis in the mouse. Proc. Natl. Acad. Sci. USA 94, 14559-14563   DOI
2 Buchholz, F., P. O. Angrand and A. F. Stewart. 1998. Improved properties of FLP recombinase evolved by cycling mutagenesis. Nat Biotechnol 16, 657-662   DOI   ScienceOn
3 Cheah, S. S. and R. R. Behringer. 2001. Contemporary gene targeting strategies for the novice. Mol. Biotechnol. 19, 297-304   DOI   ScienceOn
4 Chen, C. M. and R. R. Behringer. 2001. CREating breakthroughs. Nat Biotechnol. 19, 921-922   DOI   ScienceOn
5 Erman, B., A. S. Alag, O. Dahle, F. van Laethem, S. D. Sarafova, T. L. Guinter, S. O. Sharrow, A. Grinberg, P. E. Love and A. Singer. 2006. Coreceptor signal strength regulates positive selection but does not determine CD4/ CD8 lineage choice in a physiologic in vivo model. J. Immunol. 177(10), 6613-6625   DOI
6 Farley, F. W., P. Soriano, L. S. Steffen, and S. M. Dymecki. 2000. Widespread recombinase expression using FLPeR (flipper) mice. Genesis 28, 106-110   DOI   ScienceOn
7 Feil, R., J. Brocard, B. Mascrez, M. LeMeur, D. Metzger and P. Chambon. 1996. Ligand-activated site-specific recombination in mice. Proc. Natl. Acad. Sci. USA 93, 10887-10890   DOI   ScienceOn
8 Stemberg, N. and D. Hamilton. 1981. Bacteriophage P1 site-specific recombination.1. Recombination between LoxP sites. J. Mol. Biol. 150, 467-486   DOI
9 van der Weyden, L., D. J. Adams and A. Bradley. 2002. Tools for targeted manipulation of the mouse genome. Physiol. Genomics 11, 133-164   DOI
10 Volkert, F. C., L. C Wu, P. A. Fisher and J. R. Broach. Survival strategies of the yeast plasmid two-micron circle. Basic Life Sci. 40, 375-396
11 Behringer, R. and T. Magnuson. 2000. Tissue specific expression of Cre recombinase in mice (Special Issue). In: Genesis. Wiley-Liss. vol. 26, issue
12 Werdien, D., G. Pelier and G. U. Ryffel. 2001. FLP and Cre recombinase function on Xenopus embryos. Nucleic Acids Res. 29, e53   DOI   ScienceOn
13 Jo, D., A. Nashabi, C. Doxsee, Q. Lin, D. Unutmaz, J. Chen and H. E. Ruley. 2001. Epigenetic regulation of gene structure and function with a cell-permeable Cre recombinase. Nat. Biotechnol. 19, 929-933   DOI   ScienceOn
14 Dong, J. and G. W. Stuart. 2004. Transgene manipulation in zebrafish by using recombinase. Methods Cell Biol. 77, 363-379   DOI
15 Li M, A. K. Indra, X. Warot, J. Brocard, N. Messaddeq, S. Kato, D. Metzger and P. Chambon. 2000. Skin abnormalities generated by temporally controlled RXR mutations in mouse epidermis. Nature 407, 633-636   DOI   ScienceOn
16 Mansouri, A., P. Pla, L. Larue, and P. Gruss. 2001. Pax3 acts cell autonomously in the neural tube and somites by controlling cell surface properties. Development 128, 1995-2005
17 Peitz, M., K. Pfannkuche, K. Rajewsky and F. Edenhofer. 2002. Ability of the hydrophobic FGF and basic TAT peptides to promote cellular uptake of recombinant Cre recombinase: a tool for efficient genetic engineering of mammalian genomes. Proc. Natl. Acad. Sci. USA 99, 4489-4494   DOI   ScienceOn
18 McGuire, S. E., G. Roman and R. L. Davis. 2004. Gene expression systems in Drosophila: a synthesis of time and space. Trends Genet. 20, 384-391   DOI   ScienceOn
19 Metzger, D. and P. Chambon. 2001. Site- and time-specific gene targeting in the mouse. 1: Methods 24(1), 71-80   DOI   ScienceOn
20 Orban, P. C., D. Chui and J. D. Marth. 1992. Tissue- and site-specific DNA recombination in transgenic mice. Proc. Natl. Acad. Sci. USA. 89, 8971-8976   DOI   ScienceOn
21 Pelletier, J. and N. Sonenberg. 1989. Internal binding of eucaryotic ribosomes on poliovirus RNA: translation in HeLa cell extracts. J. Virol. 63, 441-444
22 Rodriguez, C. I., F. Buchholz, J. Galloway, R. Sequerra, J. Kasper, R. Ayala, A. F. Stewart and S. M. Dymecki. 2000. High-efficiency deleter mice show that FLPe is an alternative to Cre-LoxP. Nat. Genet. 25, 139-140   DOI   ScienceOn
23 Srivastava, V. and D. W. Ow. 2004. Maker-free site-specific gene integration in plants. Trends Biotechnol. 22, 627-629   DOI   ScienceOn
24 Fontenot, J. D, J. P. Rasmussen, L. M. Williams, J. L. Dooley, A. G. Farr and A. Y. Rudensky. 2005. Regulatory T cell lineage specification by the forkhead transcription factor foxp3. Immunity 22(3), 329-341   DOI   ScienceOn
25 Gu, H., D. Marth, P. C. Orban, H. Mossmann, and K. Rajewsky. 1994. Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science 265, 103-106   DOI   ScienceOn
26 Joshi, S. K., K. Hashimoto, and P. A. Koni. 2002. Induced DNA recombination by Cre recombinase protein transduction. Genesis 33, 48-54   DOI   ScienceOn
27 Hasty, P., A. Abuin and A. Bradley. 2000. Gene targeting, principles, and practice in mammalian cells. In: Gene Targeting: A Practical Approach (2nd ed.), edited by Joyner AL. New York: Oxford University Press, chapt. 1, p. 1-35
28 Hogan, B., R. Beddington, F. Costantini, and E. Lacy. 1994. Manipulating the mouse embryo. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
29 Indra, A. K., X. Warot, J. Brocard, J. M. Bornert, J. H. Xiao, P. Chambon and D. Metzger. 1999. Temporally-controlled site-specific mutagenesis in the basal layer of the epidermis: comparison of the recombinase activity of the tamoxifen-inducible Cre-ER(T) and Cre-ER(T2) recombinases. Nucleic. Acids. Res. 27, 4324-4327   DOI   ScienceOn
30 Kim, D. G., H. M. Kang, S. K. Jang and H. S. Shin. 1992. Construction of a bifunctional mRNA in the mouse by using the internal ribosomal entry site of the encephalomyocarditis virus. Mol. Cell. Biol. 12, 3636-3643   DOI
31 Kobayashi, T., Y. Kida, T. Kaneko, I. Pastan and K. Kobayashi. 2001. Efficient ablation by immunotoxin-mediated cell targeting of the cell types that express human interleukin-2 receptor depending on the internal ribosome entry site. J. Gene. Med. 3, 505-510   DOI   ScienceOn
32 Ahn, S. and A. L. Joyner. 2004. Dynamic Changes in the Response of Cells to Positive Hedgehog Signaling during Mouse Limb Patterning. Cell 118 (4), 505-516   DOI   ScienceOn
33 Bedell, M. A., N. A. Jenkins and N. G. Copeland. 1997. Mouse models of human disease. Part I: techniques and resources for genetic analysis in mice.1: Genes Dev.11(1), 1-10   DOI   ScienceOn