Browse > Article
http://dx.doi.org/10.5352/JLS.2007.17.4.455

Population analysis of eelgrass, Zostera marina L. in Geojedo, Gaedo, and Jedo on the southern coastal water of Korea using RAPD-PCR  

Cho, Eun-Seob (South Sea Fisheries Research Institute, NFRDI)
Lee, Sang-Yong (South Sea Fisheries Research Institute, NFRDI)
Kim, Jeong-Bae (South Sea Fisheries Research Institute, NFRDI)
Publication Information
Journal of Life Science / v.17, no.4, 2007 , pp. 455-461 More about this Journal
Abstract
Assessments of population genetic structure and diversity can be of value in formulating management plans for threatened eelgrass(Zostera maim). Using randomly amplified polymorphic DNA markers, we found evidence of significant genetic structure among the populations of eelgrass sampled at three areas(Geojedo, Gaedo, and Jedo). A highly isolated(>100 km apart) population from the Geojedo had a long genetic distance(0.16), whereas the populations from the Gaedo and Jedo(<10 km apart) exhibited far less distance(0.08). The analysis of similarity within population showed that Geojedo was over 70%, which was of lower value than of Gaedo and Jedo. Based on these results, we realized that heterogeneous population was in accordance with geographic separation. This is caused by limited seed dispersal and interrupted gene flow, although the sample size is small.
Keywords
eelgrass; gene flow; geographic scale; population structure; RAPD;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
연도 인용수 순위
1 Muniz-Salazar, R., S. L. Talbot, G. K. Sage, D. H. Ward and A. Cabello-Pasini. 2005. Population genetic structure of annual and perennial populations of Zostera marina L. along the Pacific coast of Baja Californaia and the Gulf of California. Mol. Ecol. 14, 711-722   DOI   ScienceOn
2 Muniz-Salzar, R., S. L. Talbot, C. K. Sage, D. H. Ward and A. Cabello-Pasini. 2006. Genetic structure of eelgrass Zostera marina meadows in an embayment with restricted water flow. Mar. Ecol. Prog. Ser. 107, 107-116
3 Olsen, J. L., W. T. Stam, J. A. Coyer, T. B. H. Reusch et al. 2004. North Atlantic phylogeography and large scale population differentiation of the seagrass Zostera marina L. Mol. Ecol. 13, 1923-1941   DOI   ScienceOn
4 Lee, K. S. and K. H. Dunton. 1999. Inorganic nitrogen acquisition in the seagrass Thalassia testudinum: Development of a whole-plant nitrogen budget. Limnol. Oceanogr. 44, 1204-1215   DOI   ScienceOn
5 Lee, K. S., J. I. Park, I. K. Chung, D. W. Kang and S. H. Huh. 2004. Production ecology of the seagrass Zostera marina in Jindong bay, Korea. Algae 19, 39-47   DOI   ScienceOn
6 Lee, S. M., S. Y. Lee and C. I. Choi. 2005. Reproductive phenology of four Korean seagrasses, Zostera caespitosa, Z. caulescens, Z. japonica and Z. marina. Ocean and Polar Res. 27, 125-133   DOI   ScienceOn
7 Lee, S. Y., C. J. Kwon, T. J. Kim, Y. B. Shu and C. I. Choi. 1999. Morphological variation of Zostera asiatica Miki (Zosteraceae) from various habitats. Kor. J. Environ. Biol. 17, 503-512
8 Lee, S. Y., C. J. Kwon and C. I. Choi. 2000b. Distribution of Zostera (Zosteraceae) and habitat characteristics in the eastern coastal waters of Korea. J. Kor. Fish. Soc. 33, 501-507   과학기술학회마을
9 Lee, S. Y., C. J. Kwon, S. Heo and C. I. Choi. 2000a. Seagrass (Zostera marina L., Zosteraceae) bed in the brackish lake Hwanjinpo, Korea. Kor. J. Limnol. 33, 336-341   과학기술학회마을
10 Lee, S. Y., S. M. Lee, H. G. Jee and C. I. Choi. 2001. The distribution and habitation characteristics of Zostera marina L. along the southern coast of Korea. Kor. J. Environ. Biol. 19, 313-320   과학기술학회마을
11 Lee, S. Y., C. J. Kwon, K. S. Lee and C. I. Choi. 2002. Distribution of eelgrass, Zostera marina L. on coasts of the Korean peninsula: Preliminary study for eelgrass restoration. Ocean and Polar Res. 24, 55-61   DOI   ScienceOn
12 Hamm, D. E. and R. S. Burton. 2000. Population genetics of black abalone, Haliotis cracherodii, along the central California coast. J. Exp. Mar. Bio. Ecol. 254, 235-247   DOI   ScienceOn
13 Harwell, M. C. and R. J. Orth. 2002. Long distance dispersal potential in marine macrophytes. Ecology 83, 3319-3330   DOI   ScienceOn
14 Heck, K. L. and G. S. Westone. 1977. Habitat complexity and invertebrate species richness and abundance in tropical seagrass meadows. J. Biogeoger. 4, 135-142   DOI   ScienceOn
15 Hellberg, M. E., R. S. Burton, J. E. Neigel and S. R. Palumbi. 2002. Genetic assessment of connectivity among marine populations. Bull. Mar. Sci. 70, 273-290
16 Huh, S. H. and C. L. Kitting. 1985. Trophic relationships among concentrated populations of small fishes in seagrass meadows. J. Exp. Mar. Biol. Ecol. 92, 29-43   DOI   ScienceOn
17 Kwak, S. N. and S. H. Huh. 2003. Feeding habit of Limanda yokohamae in the eelgrass (Zostera marina) bed in Kwangyang bay. J. Kor. Fish. Soc. 36, 522-527   과학기술학회마을   DOI   ScienceOn
18 Jarne, P. and P. J. L. Lagoda. 1996. Microsatellites: from molecules to populations and back. Trends in Ecol. Evol. 11, 424-429   DOI   ScienceOn
19 Kato, Y., K. Aioi, Y. Omori, N. Takahata and Y. Satta. 2003. Phylogenetic analyses of Zostera species based on rbcL and matK nucleotide sequences: Implications for the origin and diversification of seagrasses in Japanases waters. Genes Genet. Syst. 78, 329-342   DOI   ScienceOn
20 Klump, D. W., R. K. Howard and D. A. Pollard. 1989. Trophodynamics and nutritional ecology of seagrass communities. In Larkum, A. W. D., A. J. McComb and S. D. Sheperd (eds.), Biology of seagrasses: A treatiese on the biology of seagrasses with special reference to the Australian region, Elsevier Science Publishers, pp. 394-437
21 Alberte, R. S., G. K. Suba, G. Procaccini and R. C. Zimmerman. 1994. Assessment of genetic diversity of seagrass population using DNA fingerprinting: implications for population stability and management. Proc. Natl. Acad. Sci. USA 91, 1049-1053   DOI   ScienceOn
22 Baeck, G. W., S. N. Kwak and S. H. Huh. 2005. Seasonal variations in abundance and species composition of fishes in an eelgrass bed in Myoungjuri of Jindong bay. Kor. J. Ichthyol. 17, 8-18   과학기술학회마을
23 Aioi, K. 1998. On the red list of Japanese seagrasses. Aquabiology 20, 7-12
24 Choi, T. S. and K. Y. Kim. 2002. Time-dependent variation of growth and nutrient uptake of Ulva pertusa Kjellman (Chlorophyta) from intertidal eelgrass beds. Algae 17, 249-257   DOI   ScienceOn
25 Dennison, W. C, R. J. Orth, K. A. Moore, J. C. Stevenson, V. Carter, S. Kollar, P. W. Bergstrom and P. A. Batiuk. 1993. Assessing water quality with submersed aquatic vegetation. BioScience 43, 86-94   DOI   ScienceOn
26 Cowen, R. K., C. B. Paris, D. B. Olson and J. L. Fortuna. 2003. The role of long distance dispersal versus local retention in replenishing marine populations. Gulf Caribb. Res. 14, 129-137
27 de Heji, H. and P. H. Nienhuis. 1992. Intraspecific variation in isozyme patterns of phenotypically separated populations of Zostera marina L. in the south-western Netherlands. J. Exp. Mar. Biol. Ecol. 161, 1-14   DOI   ScienceOn
28 den Hartog, C. 1970. The seagrass of the world. North Holland Publication, Amsterdam, 275 p
29 Ewanchuk, P. J. and S. L. Williams. 1996. Survival and re-establishment of vegetative fragmentation of eelgrass (Zostera marina L.). Can. J. Bot. 74, 1584-1590   DOI   ScienceOn
30 Gagnon, P. S., R. I. Vadas, D. B. Burdick and B. May. 1980. Genetic identity of annual and perennial forms of Zostera marina L. Aquatic Botany 8, 157-162   DOI   ScienceOn
31 Ackerman, J. D. 1986. Mechanistic implications for pollination in the marine angiosperm Zostera marina. Aquat. Bot. 24, 343-353   DOI   ScienceOn
32 Ackerman, J. D. 1997. Submarine pollination in the marine angiosperm Zostera marina (Zosteraceae). II. Pollen transport in flow fields and capture by stigmas. Am. J. Bot. 84, 1110-1119   DOI   ScienceOn
33 Taylor, M. S. and M. E. Hellberg. 2003. Genetic evidence for local retention of pelagic larvae in a Carribean reef fish. Science 299, 107-109   DOI   ScienceOn
34 Lee, S. Y., S. M. Lee, J. H. Kim and C. I. Choi. 2003. Phenology and morphometrics change of Zostera marina L. population at Duksan port in the eastern coast of Korea. The Sea 8, 70-77
35 de Cock, A. 1980. Flowering, pollination and fruiting in Zostera marina L. Aquat. Bot. 9, 201-220   DOI   ScienceOn
36 Setchell, W.A. 1927. Zostera marina latifolia: ecad or ecotype? Bull. Torrey Bot. Club 54, 1-6   DOI   ScienceOn
37 Shin, H. C. and H. K. Choi. 1998. Taxonomy and distribution of Zostera (Zosteraceae) in eastern Asia, with special reference to Korea. Aqua. Bot. 60, 49-66   DOI   ScienceOn
38 Sneath, P. H. A. and R. R. Soka. 1973. Numerical taxonomy. Terrman, San Francisco
39 Tutin, M. A. 1936. New species of Zostera from Britain. J. Bot. 74, 227-230
40 Williams, S. L. and C. A. Davis. 1996. Population genetic analysis of transplanted eelgrass (Zostera marina) reveal reduced genetic diversity in southern California. Restor. Ecol. 4, 163-180   DOI   ScienceOn
41 Williams, S. I. and R. J. Orth. 1998. Genetic diversity and structure of natural and transplanted eelgrass populations in the Chesapeake and Chincoteague Bays. Estuaries 21, 118-128   DOI   ScienceOn
42 Williams, S. L. 2001. Reduced genetic diversity in eelgrass transplantations affects both population growth and individual fitness. Ecol. Appl. 11, 1472-1488   DOI   ScienceOn
43 Orth, R. J., K. L. Heck and J. V. Monfrans. 1984. Faunal communities in seagrass beds: A review of the influence of plant structure and prey characteristics on predator-prey relationships. Esturaries 7, 339-350   DOI   ScienceOn
44 Yun, S.G., S. H. Byun, S. N. Kwak and S. H. Huh. 2002. Seasonal variation of caprellids (Crustacea: Amphipoda) on blades of Zostera marina in Kwangyang bay, Korea. J. Kor. Fish. Soc. 35, 105-109   과학기술학회마을   DOI   ScienceOn
45 Terrado, J. and S. L. Williams. 1997. Leaf versus root nitrogen uptake by the surfgrass Phyllospadix torreyi. Mar. Ecol. Prog. Ser. 149, 267-277   DOI
46 Orth, R. J. and K. A. Moore. 1983. Chesapeake Bay: An unprecedented decline in submerged aquatic vegetation. Science 222, 51-53   DOI   ScienceOn
47 Phillips, R. C. and E. C. Menez. 1988. Seagrasses: Seagrasses. Simthsonian Contr. Mar. Sci. 34, 1-104
48 Reusch, T. B. H., W. T. Stam and J. L. Olsen. 1999. Microsatellite loci in eelgrass Zostera marina reveal marked polymorphism within and among populations. Mol. Ecol. 8, 317-321   DOI   ScienceOn
49 Reusch, T. B. H., W. T. Stam and J. L. Olsen. 2000. A microsatellite-based estimation of clonal diversity and population subdivision in Zostera marina, a marine flowering plant. Mol. Ecol. 9, 127-140   DOI   ScienceOn
50 Reusch, T. B. H. 2002. Microsatellites reveal high population connectivity in eelgrass (Zostera marina) in two contrasting coastal areas. Limnol. Oceanogr. 47, 78-85   DOI   ScienceOn
51 Ruckelshaus, M. H. 1996. Estimation of genetic neighborhood parameters from pollen and seed dispersal in the marine angiosperm Zostera marina L. Evoultion 50, 856-864
52 Lee, S. Y., S. M. Lee and C. I. Choi. 2005. Phenology and reproductive effort of two Zostera marina L. populations on the southern coast of Korea. Ocean and Polar Res. 27, 67-74   DOI   ScienceOn
53 Ruckelshaus, M. H. 1998. Spatial scale of genetic structure and an indirect estimate of gene flow in eelgrass, Zostera marina. Evolution 52, 330-343   DOI   ScienceOn
54 Rozas, L. P. and T. J. Minello. 1998. Nekton use of salt marsh, seagrass, and nonvegetated habitats in a South Texas (USA) estuary. Bull. Mar. Sci. 63, 481-501
55 Setchell, W. A. 1922. Zostera marina in its relation to temperature. Science 54, 575-577
56 Lee, S. Y., J. B. Kim and S. M. Lee. 2006. Temporal dynamics of subtidal Zostera marina and intertidal Zostera japonica on the southern coast of Korea. Marine Ecology 27, 133-144   DOI   ScienceOn
57 Les, D. H. and M. A. Cleland. 1997. Phylogenetic studies in Alismatidae, II: Evolution of marine angiosperms (seagrasses) and hydrophily. Syst. Bot. 22, 443-463   DOI   ScienceOn
58 Lesk, D. H., M. L. Moody, S. W. L. Jacobs and R. J. Bayer. 2002. Systematics of seagrasses (Zosteraceae) in Australia and New Zealand. Syst. Bot. 27, 468-484
59 Mild, S. 1932. On seagrass new to Japan. Mag. Bot. Mag. 46, 774-788   DOI
60 Miki, S. 1933. On the seagrasses in Japan (1). Zostera and Phyllospadix, with special reference to morphological and ecological characters. Bot. Mag. 47, 842-862   DOI