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http://dx.doi.org/10.5352/JLS.2007.17.3.407

Regulation of β-xylosidase biosynthesis in Paenibacillus sp. DG-22  

Lee, Tae-Hyeong (Department of Biotechnology, Dongguk University)
Lim, Pyung-Ok (Department of Science Education, Cheju National University)
Lee, Yong-Eok (Department of Biotechnology, Dongguk University)
Publication Information
Journal of Life Science / v.17, no.3, 2007 , pp. 407-411 More about this Journal
Abstract
Regulation of ${\beta}-xylosidase$ synthesis in Paenibacillus sp. DC-22 was studied to optimize the enzyme production. ${\beta}-Xylosidase$ synthesis of the Paenibacillus sp. DG-22 was observed to be regulated by carbon sources present in culture media. The synthesis of ${\beta}-xylosidase$ was induced by xylan and methyl ${\beta}-D-xylopyranoside$ (${\beta}MeXyl$) but slightly repressed by readily metabolizable monosaccharides. ${\beta}MeXyl$ was found to be the best substrate for the induction of ${\beta}$-xylosidase and the most effective induction was obtained at a concentration of 10 mg/ml. ${\beta}-Xylosidase$ production showed a cell growth associated profile with the maximum amount formed during the late exponential phase of growth. The presence of glucose and xylose decreased the level of ${\beta}-xylosidase$ activity indicating that its production was subjected to a form of carbon catabolite repression. SDS-PAGE and zymogram techniques demonstrated the induction by ${\beta}MeXyl$ and revealed the presence of one ${\beta}-xylosidase$ of approximately 80 kDa.
Keywords
Paenibacillus sp. DG-22; ${\beta}-xylosidase$; regulation;
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