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http://dx.doi.org/10.5352/JLS.2007.17.3.396

The Effects of Diesel Exhaust Particulates and Particulate Matters on the ICAM-1 and VCAM-1 Expression in the Lung of Asthma-incuced Mouse  

Li, Tian-Zhu (College of Veterinary Medicine, Konkuk University)
Lee, Soo-Jin (College of Veterinary Medicine, Konkuk University)
Jang, Yang-Ho (College of Veterinary Medicine, Konkuk University)
Lee, Jeong-Hak (College of Veterinary Medicine, Konkuk University)
Park, Se-Jong (College of Veterinary Medicine, Konkuk University)
Park, Jun-Hong (College of Veterinary Medicine, Konkuk University)
Chang, Byung-Joon (College of Veterinary Medicine, Konkuk University)
Lee, Jong-Hwan (College of Veterinary Medicine, Konkuk University)
Choe, Nong-Hoon (College of Veterinary Medicine, Konkuk University)
Publication Information
Journal of Life Science / v.17, no.3, 2007 , pp. 396-401 More about this Journal
Abstract
This research investigated whether exposure of diesel exhaust particulate (DEP) and particulate metter (PM) effect on intercellular. adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) expression in asthma-induced Balb/c and IL-10 knock out (KO) mouse. Mouse was sensitized with intraperitoneal injection with ovalbumin, followed by challenges with intranasal ovalbumin. After induction of asthma mouse placed in the inhalation chamber and exposed to DEP and PM (10 $mg/m^3$). The evidences of pulmonary inflammation were assessed by immunohistochemical stain and westen blot against ICAM-1 and VCAM-1 in the lung tissue. In the immunohistochemical stain, positive reactions for ICAM-1 and VCAM-1 were much stronger in asthma-induced groups and asthma-induced group with DEP or PM than control groups. Although mild positive reactions were appeared in asthma-induced IL-10 KO mice groups, positive reactions were very strong in the asthma-induced group with DEP or PM. In Western blot, expression of VCAM-1 was increased in asthma-induced group with DEP or PM than asthma-induced groups. In the IL-10 KO mouse, ICAM-1 and VCAM-1 expression were increased in asthma-induced group with DEP or PM than asthma-induced groups. DEP and PM exposure have additive effects on the aggravation of inflammatory signs in the asthma-induced murine model. These results suggest that inhalation of DEP and PM in asthmatic patients may aggravate clinical symptoms.
Keywords
Asthma-induced mouse; IL-10 knock out mouse; ICAM-1; VCAM-1; diesel exhaust particulate and particulate metter;
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1 Schwartz, J. and D. W. Dockery. 1992. Increased mortality in Philadelphia associated with daily air pollution concentration. Am. Rev. Respir. Dis. 145, 600-604   DOI   ScienceOn
2 Schwartz, J., D. W. Dockery and L. M. Neas. 1996. Is daily mortality associated specifically with fine particles? J. Air. Waste. Manag. Assoc. 46, 927-939   DOI   ScienceOn
3 Smith, C. H., J. Barker and T. H. Lee. 1993. Adhesion molecules in allergic inflammation. Am. Rev. Respir. Dis. 148, 75-78   DOI   ScienceOn
4 Springer, T. A. 1990. Adhesion receptors of the immune system. Nature 346, 425-434   DOI   ScienceOn
5 Springer, T. A. 1994. Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell 76, 301-314   DOI   ScienceOn
6 Wong, C. K., C. B. Wang., M. L. Li, W. K. Ip., Y. P. Tian and C. W. Lam. 2006. Induction of adhesion molecules upon the interaction between eosinophils and bronchial epithelial cell: involvement of p38 MAPK and NF-kappaB. Int. lmmunopharmacol. 6, 1859-1871   DOI   ScienceOn
7 Busse, W. Wand R F. Jr. Lemanske. 2001. Asthma. N. Engl. J. Med. 344, 350-362   DOI   ScienceOn
8 Canonica G. W., G. Ciprandi., G. P. Pesce., S. Buscaglia, F. Paolieri and M. Bagnasco. 1995. ICAM-1 on epithelial cells in allergic subjects: A hallmark of allergic inflammation. Int. Arch. Allergy. Immunol. 107, 99-102   DOI
9 Christer, J., L. Dora., G. Maria., H. B. Eythor., H. Lena and V. Per. 2005. Circulating adhesion molecules in allergic and non-allergic asthma. Respir. Med. 99, 45-51   DOI   ScienceOn
10 Ichinose, T., A. Furutama and M. Sagai. 1995. Biological effects of diesel exhaust particles (DEP) II. Acute toxicity of DEP introduced into lung by intratracheal instillation. Toxicology 99, 153-167   DOI   ScienceOn
11 Jones, D. A., L. V. Melntyre., C. W. Smith and L. J. Picker. 1994. A two-step adhesion cascade fur T cell/endothelial cell interactions under flow conditions. J. Clin. Invest. 94, 2443-2456   DOI   ScienceOn
12 Lein, R. R. and C. Wegner. 1992. Role of intercellular adhesion molecule-1 in the inflammatory response. Kidney Int. 41, 617-619   DOI   ScienceOn
13 Salma, I., M. Willy., Z. P. Eva and A. Gyula. 2001. Comprehensive characteri sation of atmospheric aerosols in Budapest, Hungary: physicochemical properties of inorganic species. Atmos. Environ. 35, 4367-4378   DOI   ScienceOn
14 Peterson, B. 1996. Saxon. Global increases in allergic respiratory disease: the possible role of diesel exhaust particles. Ann. Allergy Asthma Immunol. 77, 263-270   DOI   ScienceOn
15 Pope, C. A. III., D. V. Bates and M. E. Raizenne. 1995. Health effects of particulate air pollution: time for reassessment? Environ. Health Perspect. 103, 472-480   DOI   ScienceOn
16 Ruoslahti, E. 1991. Integrins. J. Clin. Invest. 87, 1-5   DOI   ScienceOn
17 Albelda, S. M., C. W. Smith and P. Ward. 1994. Adhesion molecules and inflammatory injury. FASEB J. 8, 504-512   DOI
18 Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254   DOI   ScienceOn
19 Alon, R., P. D. Kassner., M. Woldemar., E. B. Finger., M. E. Hemler and T. A. Springer. 1995. The integrin VLA-4 supports tethering and rolling in flow on VCAM-1. J. Cell. BioI. 128, 1243-1256   DOI   ScienceOn
20 Boulet, L. P., H. Turcotte., M. Laviolette., F. Naud., M. C. Bernier., S. Martel and J. Chakir. 2000. Airway hyper-responsiveness, inflammation, and subepithelial collagen deposition in recently diagnosed versus long-standing mild asthma. Influence of inhaled corticosteroids. Am. J. Respir. Crit. Care. Med. 162, 1308-1313   DOI   ScienceOn
21 Bunn, W. B. III., P. A. Valberg., T. J. Slavin and C. A. Lapin. 2002. What is new in diesel. Int. Arch. Occup. Environ. Health. 75, 122-132   DOI   ScienceOn
22 Kim, S. C. and S. H. Byun. 2005. The effects of old-enlandiae diffusae herba extract on eosinophil, IgE and IL-4 in experimental asthma induced by ovalbumin. Kor. J. Herbology 20, 35-42