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http://dx.doi.org/10.5352/JLS.2007.17.3.305

Glucocorticoid Regulation of Gene Expression in Hippocampal CA3 and Dentate Gyrus  

Kim, Dong-Sub (School of Applied and Life Science, College of National Resources and Life Science, Pusan National University)
Ahn, Soon-Cheol (Department of Microbiology and Immunology, School of Medicine, Pusan National University)
Kim, Young-Jin (Department of Microbiology and Immunology, School of Medicine, Pusan National University)
Park, Byoung-Keun (Bio21 Center)
Ahn, Yong-Tae (Korea Basic Science Institute)
Kim, Ji-Youn (Korea Basic Science Institute)
Kyoji, Morita (Department of Pharmacolosy, Tokushima University School of Medicine)
Her, Song (Korea Basic Science Institute)
Publication Information
Journal of Life Science / v.17, no.3, 2007 , pp. 305-311 More about this Journal
Abstract
Glucocorticoids (GCs) alter metabolism, synaptogenesis, apoptosis, neurogenesis, and dendritic morphology in the hippocampus. To better understand how glucocorticoids regulate these aspects of hippocampal biology, we studied gene expression patterns in the CA3 (Hippocampal pyramidal cell field CA3) and dentate gyrus (DG). Litter-matched Lewis inbred rats treated for 20 days with either 9.5 mg per day sustained-release corticosterone or placebo pellets were compared with high-density oligonucleotide microarray analysis (Rat Neurobiology U34 Arrays, Affymetrix). In placebo-treated rats, 32 genes were expressed at greater levels in CA3 than DG, whereas 3 genes were expressed at great levels in DC than CA3. Regional differences were also apparent in corticosterone-induced changes in the hippocampal transcriptome. Six genes in CA3 and 41 genes in DC were differentially regulated by corticosterone. As per the glucocorticoid effects on gene transcription in the brain, forty three of these genes were upregulated, and 4 genes were downregulated. Genes differentially expressed in hippocampus included those for 13 neurotransmitter proteins, 5 ion channel related proteins, 4 transcription factors, 3 neurotrophic factors, 1 cytokine, 1 apoptosis related protein, and 5 genes involved in synaptogenesis. Interestingly, GCs can have suppressive effects on brain BDNF mRNA transcription, one of the neurotrophic factors. These results indicate the diversity of targets affected by chronic exposure to corticosterone and highlight important regional differences in hippocampal neurobiology.
Keywords
Corticosterone; transcripts; oligonucleotide microarray; CA3; DG; rat; BDNF;
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