Browse > Article
http://dx.doi.org/10.5352/JLS.2007.17.2.248

The Effects of Diesel Exhaust Particulates and Particulate Matters on the Airway Remodeling in the Asthma-induced Mice  

Li, Tianzhu (Konkuk University College of Veterinary Medicine)
Lee, Soo-Jin (Konkuk University College of Veterinary Medicine)
Jang, Yang-Ho (Konkuk University College of Veterinary Medicine)
Park, Jun-Hong (Konkuk University College of Veterinary Medicine)
Park, Se-Jong (Konkuk University College of Veterinary Medicine)
Lee, Jeong-Hak (Konkuk University College of Veterinary Medicine)
Choe, Nong-Hoon (Konkuk University College of Veterinary Medicine)
Publication Information
Journal of Life Science / v.17, no.2, 2007 , pp. 248-253 More about this Journal
Abstract
This research investigated whether exposure of diesel exhaust particulate (DEP) and particulate matter (PM) effects on airway remodeling in asthma induced Balb/c and IL-10 knock out (KO) mouse. Mice were sensitized with intraperitoneal injection with ovalbumin, followed by challenges with intranasal ovalbumin. After that mice placed in inhalation chamber and exposed to DEP and $PM(10\;mg/m^3)$. The evidence of airway remodeling was assessed by masson's trichrome staining and PAS staining. The stainability of masson's trichrome and PAS reaction were increased in asthma-induced Baltic mice groups compared with control mice groups. More intensive stainability for masson's trichrome and PAS were appeared in the asthma-induced DEP and PM-exposed groups than asthama-induced groups. But, not significantly increased subepithelial fibrosis and the nember of goblet cell hyperplasia in asthma-induced IL-10 KO mice groups and asthma-induced+DEP and PM-exposed IL-10 KO mice than IL-10 KO mice groups. These results indirectly suggesting that exposure to DEP and PM in asthmatic patients might be aggravate clinical symptoms and IL-10 which seems to play a central role in allergic asthma. In conclusion, DEP and PM exposure might have additive effects on the ovalbumin- induced asthma in a murine model.
Keywords
Asthma-induced mouse; IL-10 KO mouse; Airway remodeling; DEP and PM;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Dockery, D. W., F. E. Speizer, D. O. Stram, J. H. Ware, J. D. Spengler and B. G. Jr. Ferris. 1989. Effects of inhalable particles on respiratory health of children. Am. Rev. Respir. Dis. 139, 587-594   DOI   ScienceOn
2 Redington, A. E. and P. H. Howarth. 1997. Airway wall remodeling in asthma. Thorax. 52, 310-312   DOI   ScienceOn
3 Peterson, B. and A. Saxon. 1996. Global increases in allergic respiratory disease: the possible role of diesel exhaust particles. Ann. Allergy. Asthma. Immunol. 77, 263-270   DOI   ScienceOn
4 Pope, C. A. III., D. V. Bates and M. E. Raizenne. 1995. Health effects of particulate air pollution : time for reassessment? Environ. Health. Perspect. 103, 472-480   DOI   ScienceOn
5 Pope, C. A. III., D. W. Deckery, J. D. Spengler and M. E. Raizenne. 1991. Respiratory health PM10 pollution. A daily time series analysis. Am. Rev. Respir. Dis. 144, 668-674   DOI   ScienceOn
6 Schwartz, J. and D. W. Dockery. 1992. Increased mortality in philadelphia associated with daily air pollution concentration. Am. Rev. Respir. Dis. 145, 600-604   DOI   ScienceOn
7 Pope, C. A. III. and R. E. Kanner. 1993. Acute effects of PM10 pollution on pulmonary function of smokers with mild to moderate COPD. Am. Rev. Respir. Dis. 147, 1336-1340   DOI   ScienceOn
8 Roche, W. R., R. Reasley, J. H. Williams and S. T. Holgate. 1989. Subepithelial fibrosis in the bronchi of asthmatics. Lancet 1, 520-524
9 Schwartz, J. 1994. Air pollution and daily mortality: a review and meta analysis. Environ. Res. 64, 36-52   DOI   ScienceOn
10 Fish, J. E. and S. P. Peters. 1999. Airway remodeling and persistent airway obstruction in asthma. J. Allergy. Clin. Immunol. 104, 509-516   DOI   ScienceOn
11 Garvey W. Modified elastic tissue-Masson trichrome stain. 1984. Stain. Tech
12 Kips, J. C. and R. A. Pauwels. 1999. Airway wall remodeling: does it occur and what does in mean? Clin. Exp. Allergy 29, 1457-1466   DOI   ScienceOn
13 Harre, E. S. M., P. D. Price and R. B. Ayrey. 1997. Respiratory effects of air pollution in chronic obstructive pulmonary disease: a three-month prospective study. Thorax. 52, 1040-1044   DOI   ScienceOn
14 Ichinose, T., A. Furutama and M. Sagai. 1995. Biological effects of diesel exhaust particles (DEP) II. Acute toxicity of DEP introduced into lung by intratracheal instillation. Toxicology 99, 153-167   DOI   ScienceOn
15 Kim, S. C. and S. H. Byun. 2005. The effects of old¬enlandiae diffusae herba extract on eosinophil, IgE and IL-4 in experimental asthma induced by ovalbumin. Kor. J. Herbology 20(2), 35-42
16 Laprise, C., M. Laviolette, M. Boutet and L. P. Boulet. 1999. Asymptomatic airway hyperresponsiveness: relationships with airway inflammation and remodeling. Eur. Respir. J. 14, 63-73   DOI   ScienceOn
17 Lebowitz, M. D. 1996. Epidemiological studies of the respiratory effects of air pollution. Eur. Respir. J. 9, 1029-1054   DOI   ScienceOn
18 Leigh, R., R. Ellis, J. Wattie, D. S. Southam, M. De Hoogh, J. Gauldie, P. M. O' Byrne and M. D. Inman. 2002. Dysfunction and remodeling of the mouse airway persist after resolution of acute allergen-induced airway inflammation. Am. J. Respir. Cell. Mol. Biol. 27, 526-535   DOI   ScienceOn
19 McManus J.F.A. 1946. Histological demonstration of mucin periodic acid. Nature 158, 202
20 Aikawa, T., S. Shimura, H. Sasaki, M. Ebina and T. Takishima. 1992. Marked goblet cell hyperplasia with mucus accumulation in the airways of patients who died of severe acute asthma attack. Chest 101, 916-921   DOI   ScienceOn
21 Bousquet, J., P. Chanez, J. Y. Lacoste, R. White, P. Vic, P. Godard and F. B. Michel. 1992. Asthma: a disease remodeling the airways. Allergy 47, 3-11
22 Bunn, W. B. III., P. A. Valberg, T. J. Slavin and C. A. Lapin. 2002. What is new in diesel. Int. Arch. Occup. Environ. Health 75, 122-132   DOI   ScienceOn
23 Davies, D. E., J. Wicks, R. M. Powell, S. M. Puddicombe and S. T. Holgate. 2003. Airway remodeling in asthma: New insights. J. Allergy. Clin. Immunol. 111, 215-225   DOI   ScienceOn
24 Dockery, D. W., C. A. Pope, X. Xu, J. D. Spengler, J. H. Ware, M. E. Fay, B. G. Jr. Ferris and F. E. Speizer. 1993. An association between air pollution and mortality in six US cities. N. Engl. J. Med. 329, 1753-1759   DOI   ScienceOn
25 Dockery, D. W. and C. A. III. Pope. 1994. Acute respiratory effects of particulate air pollution. Annu. Rev. Public. Health. 15, 107-132   DOI   ScienceOn
26 Emanuel, M. B. 1988. Hay fever, a post industrial revolution epidemic: a history of its growth during the 19th century. Clin. Allergy 18, 295-304   DOI
27 Bascom, R., P. A. Bromberg and D. L. Costa. 1996. Health effects of outdoor air pollution. Am. J. Respir. Crit. Care. Med. 153, 477-498   DOI   ScienceOn