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http://dx.doi.org/10.5352/JLS.2007.17.2.241

Cell Surface Display of Cycloinulooligosaccharide Fructanotransferase Gene in Saccharomyces cerevisiae  

Kim, Hyun-Jin (Department of Biomaterial Control, Dong-Eui University)
Lee, Jae-Hyung (Department of Biomaterial Control, Dong-Eui University)
Kim, Hyun-Chul (Bioneer Corp.)
Kim, Yeon-Hee (Department of Biotechnology, Osaka University)
Kwon, Hyun-Ju (Department of Biomaterial Control, Dong-Eui University, Department of Life Science & Biotechnology, Dong-Eui University)
Nam, Soo-Wan (Department of Biomaterial Control, Dong-Eui University)
Publication Information
Journal of Life Science / v.17, no.2, 2007 , pp. 241-247 More about this Journal
Abstract
The cycloinulooligosaccharide fructanotransferase (CFTase) gene (cft) from Paenibacillus macerans was subcloned into the surface display vector, pCTcon (GAL1 promoter). The constructed plasmid, pCTECFTN (9.0 kb) was introduced to S. cerevisiae EBY100 cell and then east transformants were selected on the synthetic defined medium lacking uracil and on the inulin containing medium. The surface display of CFTase was confirmed by immunofluorescence microscopy and its enzymatic ability to form cycloinulooligosaccharides(cyclofructans, CFs) from inulin. The total activity of the CFTase was reached about 5.52 unit/1 by cultivation of yeast transformant on YPDG medium. The optimized conditions determined were as follows; pH, 8.0; temperature, $50^{\circ}C$ ; substrate concentration, 5%; inulin source, Jerusalem artichoke. By the reaction with inulin, CFs consisting of cycloinulohexaose (CF6), cycloinuloheptaose (CF7), and cycloinulooctaose (CF8) were produced and CF6 was the major product.
Keywords
Cycloinulooligosaccharide fructanotransferase; Saccharomyces cerevisiae; cell surface display; cyclofructan; inulin;
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1 Jin, Z., J. Wang, B. Jiang and X. Xu. 2005. Production of inulooligosaccharides by endoinulinases from Aspergillus ficuum. Food Res. Int. 38, 301-308   DOI   ScienceOn
2 Kim, H. Y. and Y. J. Choi. 2001. Molecular characterization of cycloinulo- oligosaccharide fructanotransferase from Bacillus macerans. Appl. Environ. Microbiol. 67, 995-1000   DOI   ScienceOn
3 Kondo, A. and M. Ueda. 2004. Yeast cell-surface display-applications of molecular display. Appl. Microbiol. Biotechnol. 64, 28-40   DOI   ScienceOn
4 Bader, E. T. and K. D. Wittrup. 1997. Yeast surface display for screening combinatorial polypeptide libraries. Nat. Biotechnol. 15, 553-557   DOI   ScienceOn
5 Kim, K. Y., M. D. Kim, N. S. Han and J. H. Seo. 2002. Display of Bacillus macerans cyclodextrin glucanotransferase on cell surface of Saccharomyces cerevisiae. J. Microbiol. Biotechnol. 12, 411-416
6 Washida M., S. Takahachi, M. Ueda and A. Tanaka. 2001. Spacer-mediated display of active lipase on the yeast cell surface. Appl. Microbiol. Biotechnol. 56, 681-686   DOI   ScienceOn
7 Duagherty, P. S., M. J. Olsen, B. L. Linverson and G. Georgiou. 1999. Development of an optimized expression system for the screening of antibody libraries displayed on the Esherichia coli surface. Protein Eng. 12, 613-621   DOI
8 Georgiou, G., H. L. Poetschke, C. Stathopoulos and J. A. Francisco. 1993. Practical applications of engineering Gram-negative bacterial cell surfaces. Trends Biotechnol. 11, 6-10   DOI   ScienceOn
9 Valentin, E., E. Herrero, J. F. I. Pastor and R. Sentandreu. 1984. Solubilization and analysis of mannoprotein molecules from the cell wall of Saccharomyces cerevisiae. Arch. Microbiol. 130, 1419-1428   DOI
10 van der Vaart, J. M., L. H. P. Caro, J. W. Chapman, F. M. Klis and C. T Verrips. 1995. Identification of three mannoproteins in the cell wall of Saccharomyces cerevisiae. J. Bacteriol. 177, 3104-3110   DOI
11 Sawada, M., T. Tanaka, Y. Takai, T. Hanafrsa, T. Taniguchi, M. Kawamura and T. Uchiyama. 1991 The srystal structure of cyclo-inulohexaose produce from inulin by cycloinulooligosaccharide fructanotransferase. Carbohydr. Res. 217, 7-17   DOI   ScienceOn
12 Lipke, P. Nand J. Kurjan. 1992. Sexual agglutination in budding yeasts: Structure, function, and regulation of adhesion glycoproteins. Microbiol. Rev. 56, 180-194
13 Little, M., P. Fuchs, F. Breitling and S. Dubel. 1993. Bacterial surface presentation of proteins and peptides: and alternative to phage technology. Trends Biotechnol. 11, 3-5   DOI   ScienceOn
14 Romanos, M. A., C. A. Scorer and J. J. Clare. 1992. Foreign gene expression in yeast : a review. Yeast 8, 423-488   DOI   ScienceOn
15 Schmid, G. 1989. Cyclodextrin glycosyltransferase production; yield enhancement by overexpression of cloned genes. Trends Biotechnol. 7, 244-248   DOI   ScienceOn
16 Schreuder, M. P., A. T. Mooren, H. Y. Toschka, C. T. Verrips and F. M. Klis. 1996. Immobilizing proteins on the surface of yeast cells. Trends Biotechnol. 14, 115-120   DOI   ScienceOn
17 Schreuder, M. P., C. Deen, W. J. A. Boersma, P. H. Pouwels and F. M. Klis. 1996. Yeast expressing hepatitis B virus surface antigen determinants on its surface: implications for a possible oral vaccine. Vaccine 14, 383-388   DOI   ScienceOn
18 Takai, Y., Y. Okumura, S. Takahashi, M. Sawada, M. Kawamura and T. Uchiyama. 1993. A permethylated cyclic fructo-oligosaccharide host that can bind cation in solution. J. Chem. Soc. Chem. Commun. 1, 53-54
19 Uchiyama, T., M. Kawamura, T. Uragami and H. Okuno. 1993. Complexing of cycloinulo- oligosaccharides with metal ions. Carbohydr. Res. 241, 245-248   DOI   ScienceOn
20 Ohta, K., S. Hamada and T. Nakamura. 1993. Production of high concentrations of ethanol from inulin by simultaneous saccharification and fermentation using Aspergillus niger and Saccharomyces cerevisiae. Appl. Environ. Microbiol. 59, 729-733
21 Kawamura, M., T. Uchiyama, T. Kuramoto, Y. Tamura and K. Mizutani. 1989. Formation of a cycloinulooligosaccharide from inulin by an extracellular enzyme of Bacillus circulans OKUMZ31B. Carbohydr. Res. 192, 83-90   DOI   ScienceOn
22 Vandamme, E. J. and D. G. Derycke. 1983. Microbial inulinase: fermentation process, properties, and applications. Adv. Appl. Microbiol. 29, 139-176   DOI
23 Ito, H., Y. Fukuda, K. Murata and A. Kimura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153, 163-168
24 Jeon, S. J., D. J. You, H. J. Kwon, S. Kanaya, N. Kunihiro, K. H. Kim, Y. H. Kim and B. W. Kim. 2002. Cloning and characterization of cycloinulooligosaccharide fructanotransferase (CFTase) from Bacillus polymyxa MGL21. J. Microbiol. Biotechnol. 12, 921-928
25 Kawamura, M. and T. Uchiyama. 1993. Reaction catalyzed by cycloinulooligosaccharide fructanotransferase. Biosci. Biotechnol. Biochem. 57, 343-350   DOI
26 Kawamura, M., H. Nakai, T. Uchiyama, Y. Takai and M. Sawada.1997. Synthesis of methyl 6-O-${\beta}$-inulotriosyl-${\alpha}$ -D-glucopyranoside by intermolecular transglycosylation reaction of cycloinulo-oligosaccharide fructanotransferase, Carbohydr. Res. 297, 187-190   DOI   ScienceOn
27 Kim, D. H., Y. J. Choi, S. K. Song and J. W. Yun. 1997 . Production of oligo- saccharides using endo-inulinase from Pseudomonas sp. Biotechnol. Lett. 19, 369-371   DOI   ScienceOn
28 Kim, H. C., J. H. Kim, S. J. Jeon, W. B. Choi and S. W. Nam. 2005. Expression of Paenibacillus macerans Cycloinulooligosaccharide Fructanotransferase in Saccharomyces cerevisiae. J. Life Sci. 15, 317-322   과학기술학회마을   DOI   ScienceOn