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http://dx.doi.org/10.5352/JLS.2007.17.2.179

High Level Production of human Protein Tyrosine Kinase-6 in Insect Cells Using Drosophila Peptidoglycan Recognition Protein-LB as a fusion protein  

Kim, Seul-Ki (College of Pharmacy and Research Institute for Drug Development, Pusan National University)
Kim, Han-Ie (Department of Biochemistry, College of science, Yonsei University)
Woo, Jae-Sung (Department of Life Science, Pohang University of Science and Technology)
Cho, Hyun-Soo (Department of Biology, College of science, Yonsei University)
Jung, Yun-Jin (College of Pharmacy and Research Institute for Drug Development, Pusan National University)
Lee, Seung-Taek (Department of Biochemistry, College of science, Yonsei University)
Ha, Nam-Chul (College of Pharmacy and Research Institute for Drug Development, Pusan National University)
Publication Information
Journal of Life Science / v.17, no.2, 2007 , pp. 179-184 More about this Journal
Abstract
PTK6, an intracellular protein tyrosine kinase, is significantly overexpressed in a majority of breast cancers and has a role in promoting the proliferation of the cancer cells, but not of normal cells. Here, we report high-level production of the catalytic unit of PTK6 fused with Drosophila peptidoglycan recognition protein (PGRT)-LB, in the baculovirus system. We first found that the PGRP-LB was potentially useful as a fusion partner to increase the yield of heterologous protein in the baculovirus system. The purified recombinant protein exhibited a 1.5-fold activity with much higher yield than the bacterially-expressed protein. The protein expressed in the baculovirus system will be useful for the crystallization to determine its crystal structure helping understand the molecular mechanism of PTK6 and design its inhibitors.
Keywords
PTK6; overexpression; recombinant protein; baculovirus;
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