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http://dx.doi.org/10.5352/JLS.2007.17.1.070

Isolation and characterization of a marine bacterium Thalassomonas sp. SL-5 producing β-agarase  

Lee, Dong-Geun (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Kim, Nam-Young (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Jang, Min-Kyung (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Lee, Ok-Hee (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Publication Information
Journal of Life Science / v.17, no.1, 2007 , pp. 70-75 More about this Journal
Abstract
A novel agar-degrading bacterium SL-5 was isolated from seashore of Homigot at Kyung-Buk province, and cultured in marine broth 2216 media. The bacterium SL-5 was identified as Thalassomonas genus by 16S rDNA sequencing with 96% identity. Growth rate was faster at $27^{\circ}C$ than at $37^{\circ}C$ and agarase was produced as growth-related. The optimum pH of the enzyme activity was 7.0 and the optimum temperature for the reaction was $40^{\circ}C$. Although the enzyme had no thermostability, the enzyme activity was remained over 80% at $60^{\circ}C$. The enzyme hydrolyzed neoagarohexaose to yield neoagarobiose as the main product, indicating that the enzyme is $\beta-agarase$. Thus, the enzyme would be useful for the industrial production of neoagarobiose.
Keywords
$\beta-agarase$; marine; Thalassomonas sp.;
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