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http://dx.doi.org/10.5352/JLS.2007.17.1.045

Biological Activity of Tumor Necrosis Factor-α Secreted from Smooth Muscle Cell Overexpressing FADD  

Kim, Sun-Mi (Department of Pharmacology, School of Medicine, Pusan National University)
Lee, Kyeong-Ah (Department of Pharmacology, School of Medicine, Pusan National University)
Kim, Koan-Hoi (Department of Pharmacology, School of Medicine, Pusan National University)
Publication Information
Journal of Life Science / v.17, no.1, 2007 , pp. 45-50 More about this Journal
Abstract
This study investigated biological activity of tumor necrosis factor $(TNF)-\alpha$ secreted from smooth muscle cell (SMC) destined for death by expressing Fas associated death domain containing protein (FADD) (FADD-SMC) when the cells are grown without tetracycline in culture medium. In the absence of tetracycline the FADD-SMC secreted approximately 1000 pg/ml $TNF-\alpha$, whereas hardly detectable amount of the cytokine existed in the presence of tetracycline. The culture medium collected from the FADD-SMC grown in the absence of tetracycline increased phosphorylated form of p38 MAPK and up-regulated nuclear factor kappa B (NF-kB). The medium collected without tetracycline also caused death of L929 cells. Depletion of $TNF-\alpha$ with the soluble TNF receptor (sTNFR) inhibited the phosphorylation of p38 MAPK, the up-regulation of NF-kB activity and the death activity of the medium collected from FADD-SMC in the absence of tetracycline. These results indicate that $TNF-\alpha$ secreted from SMC undergoing death is biologically active and can affect cellular function.
Keywords
Apoptosis; p38 MAPK; Smooth muscle cell; Tumor necrosis $factor-\alpha$;
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