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http://dx.doi.org/10.5352/JLS.2007.17.1.162

PCR Specific Primer for the Detection of Vibrio tapetis  

Kim, Yeong-Jin (Department of Microbiology and Immunology, College of Medicine, Pusan National University)
Lee, Sun-Yi (Department of Microbiology and Immunology, College of Medicine, Pusan National University)
Cho, Hyo-Jin (Department of Microbiology and Immunology, College of Medicine, Pusan National University)
You, Sun-Nyung (Department of Microbiology and Immunology, College of Medicine, Pusan National University)
Kim, Cheol-Min (Department of Biochemistry, College of Medicine, Pusan National University)
Choi, Yong-Lark (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-a University)
Park, Byoung-Keun (Bio21 Center)
Ahn, Soon-Cheol (Department of Microbiology and Immunology, College of Medicine, Pusan National University)
Publication Information
Journal of Life Science / v.17, no.1, 2007 , pp. 162-165 More about this Journal
Abstract
Brown Ring Disease (BRD) is a bacterial disease caused by Vibrio tapetis which affects cultured clam Ruditapes philippinarum and causes heavy economic losses on Atlantic coasts of france, Spain and England. In this study, to evaluate the effective detection of the pathogen, specific primer set based on 16S ribosomal RNA (rRNA) sequences designed for rapid detection of V. tapetis. Polymerase chain reaction (PCR) with this primer set produced the specific band for each V. tapetis. The length of PCR product using designed primer set of Vbts-F and Vbts-R was about 400 bp. Therefore, these primers will be provided with a basic tool for rapid detection of V. tapetis in the various cases such as examination of imported aquatic products, diagnosis of aquatic organisms, and etc.
Keywords
BRD; Vibrio tupetis; 16S rRNA; PCR; specific primer;
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