Browse > Article
http://dx.doi.org/10.5352/JLS.2007.17.1.150

Microarray Analysis of Alteration in Gene Expression by Acori graminei rhizoma (AGR) Water-Extract in a Hypoxic Model of Cultured Rat Cortical Cells  

Park, Dong-Jun (Dept. of Oriental Medicine, Dongguk University)
Jung, Seung-Hyun (Dept. of Oriental Medicine, Dongguk University)
Moon, Il-Soo (Department of Anatomy, Dongguk University)
Lee, Won-Chol (Dept. of Oriental Medicine, Dongguk University)
Shin, Gil-Jo (Dept. of Oriental Medicine, Dongguk University)
Publication Information
Journal of Life Science / v.17, no.1, 2007 , pp. 150-161 More about this Journal
Abstract
Acori graminei Rhizomn (AGR) is a perennial herb which has been used clinically as a traditional oriental medicine against stroke, Alzheimer's disease, and vascular dementia. We investigated the effect of AGR on the modulation of gene expression profile in a hypoxic model of cultured rat cortical cells. Rat cerebrocortical cells were grown in Neurobasal medium. On DIV12, cells were treated with AGR $(10ug/m\ell)$, given a hypoxic shock (2% $O_2$, 3 hr) on DIV14, and total RNAs were prepared one day after shock. Microarray analyses indicated that the expression levels of most genes were altered within the global M values +0.5 and -0.5, i.e., 40% increase or decrease. There were 750 genes which were upregulated by < global M +0,2, while 700 genes were downregulated by > global M -0.2. The overall profile of gene expression suggests that AGR suppresses apoptosis (upregulation of anti-apopotic genes such as TEGT, TIEG, Dad, p53, and downregulation of pro-apopotic genes such as DAPK, caspase 2, pdcd8), ROS (upregulation of RARa, AhR), and that AGR has neurotrophic effects (upregulation of Aktl, Akt2). These results provide a platform for investigation of the molecular mechanism of the effect of AGR in neuroprotection.
Keywords
Acori graminei Rhizoma; rat cortical cell culture; hypoxia; microarray;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Watson, A., A. Mazumder, M. Stewart and S. Balasubramanian. 1998. Technology for microarray analysis of gene expression. Curr Opin Biotechnol. 9, 609-614   DOI   ScienceOn
2 Whitney, L.W., K. G. Becker, N. J. Tresser,C. I. Caballero-Ramos, P. J. Munsun, V. V. Prabhu, J. M. Trent, H. F. McFarland and W. E. Biddison. 1999. Analysis of gene expression in multiple sclerosis lesions using cDNA microarrays. Ann Neurol. 46, 425-428   DOI   ScienceOn
3 Widlak, P. 2004. DNA microarrays, a novel approach in studies of chromatin structure. Acta Biochim Pol. 51, 1-8
4 Xu, Q., J. C. Reed. 1998. Bax inhibitor-1, a mammalian apoptosis suppressor identified by functional screening in yeast. Mol. Cell. 1, 337-346   DOI   ScienceOn
5 Yukawa, K., K. Hoshino, M. Kishino, M. Mune, N. Shirasawa, A. Kimura, Y. Tsubota, K. Owada-Makabe, T. Tanaka, M. Ichinose, M. Maeda, K. Takeda and S. Akira. 2004. Deletion of the kinase domain in death-associated protein kinase attenuates renal tubular cell apoptosis in chronic obstructive uropathy. Int J. Mol Med. 13, 515-520
6 Zimmermann, K. C., D. R. Green. 2001. How cells die: Apoptosis pathways. J. Allergy Clin Immunol. 108, 99-103   DOI   ScienceOn
7 Son S. Y. 1979. sin-nong-bon-cho-gyung, pp. 38-39, Moonguangdoseoyuhangongsa, Taipei
8 Lee S. J. 1999. Bon-cho-kang-mok, pp. 410-411, Science techonology book publisher. Baijing
9 Wang-Ang. 1989. Bon-cho-bi-yo, pp. 44-45, Komoonsa. Seoul
10 Hco. J. 1999. Dong-Eui-Bo-Gam, pp. 965, 961, 1955. Bubinbooks. Seoul
11 Nie, M., A. L. Blankenship and J. P. Giesy. 2001. Interactions between aryl hydrocarbon receptor(AhR) and hypoxia signaling pathways. Environ. Toxicol. Pharmacol. 10, 17-27   DOI   ScienceOn
12 Ramsay, G. 1998. DNA chips: state-of-the art. Nature Biotechnol. 16, 40-44   DOI   ScienceOn
13 Ribeiro, A., S. F. Bronk, P. J. Roberts, R. Urrutia and G. J. Gores. 1999. The ransforming growth factor beta(1)-inducible transcription factor TIEG1, mediates apoptosis through oxidative stress. Hepatology 30, 1490-1497   DOI
14 Rosenbaum, D. M., M. Michaelson, D. K. Batter, P. Doshi and J. A. Kessler. 1994. Evidence for hypoxia-induced, programmed cell death of cultured neurons. Ann Neurol. 36, 864-870   DOI   ScienceOn
15 Schena, M., R. A. Heller, T. P. Theriault, K. Konrad, E. Lachenmeier and R. W. Davis. 1998. Microarrays: biotechnology's discovery platform for functional genomics. Trends Biotechnol. 16, 301-306   DOI   ScienceOn
16 Schwartzman, R. A., J. A. Cidlowski, 1993. Apoptosis: the biochemistry and molecular biology of programmed cell death. Endocrinol. 14, 133-151
17 Semenza, G. L. 1998. Hypoxia-inducible factor 1: master regulator of $O_2$ homeostasis. Curr. Opin. Genet. Dev. 8, 588-594   DOI   ScienceOn
18 Semenza, G. L., P. H. Roth,H. M. Fang and G. L. Wang. 1994. Transcriptional regulation of genes encoding glycolytic enzymes by hypoxia-inducible factor 1. J. Biol Chem. 269, 23757-23763
19 Semenza. G. L., B. H. Jiang, S. W. Leung, R. Passantino, J. P. Concordet, P. Maire and Giallongo A. 1996. Hypoxia response elements in the aldolase A, enolase 1, and lactate dehydrogenase A gene promoters contain essential binding sites for hypoxia-inducible factor 1. J. Biol Chem. 271, 32529-32537   DOI   ScienceOn
20 Seo, G. S. 2003. Effects of Acori graminei rhizorna(石菖蒲) on the Modulation of ROS, MMP, in a Hypoxic Model of Cultured Rat Cortical Cells. Dongguk graduate school
21 Kim, G. O., H. J. Kim, G. H. Park, G. H. Seok, S. Y. Kim, D. K. Ahn and H. C. Kim. 2000. Neuroprotective Effects of Acori graminei mizoma on the Brain Ischemia induced by Four - Vessel Occlusion in Rats. The Korea Association of Herbology 15, 79-85   과학기술학회마을
22 Kirino, T. 1982. Delayed neuronal death in the gerbil hippocampus following ischemia. Brain Res. 239, 57-69   DOI   ScienceOn
23 Kirino, T. 1982. Delayed neuronal death in the gerbil hippocampus following ischemia. Brain Res. 239,57-69   DOI   ScienceOn
24 Kirino, T., A. Tamura and K. Sano. 1985. Selective vulnerability of the hippocampus to ischemia-reversible and irreversible types of ischemic cell damage. Progress in Brain Res. 63, 39-58   DOI
25 Kobori, N., G. L. Clifton and P. Dash. 2002. Altered expression of novel genes in the cerebral cortex following experimental brain injury. Brain Res. Mol. Brain Res. 104, 148-158   DOI   ScienceOn
26 Lee, U. J., G. N. Park S. G. Jeong, Y. Jun and S. G. Kim. 2003. Original Articles : Change in the Intrathecal Cytokine level in Hypoxic-ischemic Encephalopathy after Cardiac Arrest. The Korean society of Emergency Medicine 14, 494-498
27 Gonzalez, F.J., P. Fernandez-Salguero. 1998. The aryl hydrocarbon receptor: studies using the AHR-null mice. Drug Metab Dispos. 26, 1194-1198
28 Levy, A.P., N. S. Levy, S. Wegner and M. A. Goldberg. 1995. Transcriptional regulation of the rat vascular endothelial growth factor gene by hypoxia. J. Biol Chem. 270, 13333-13340   DOI   ScienceOn
29 Martin, L. J., A. M., Brambrink, C Lehmann, C Portera-Cailliau, R. C. Koehler, J. Rothstein and R. J. Traystman. 1997. Hypoxia-ischemia causes abnormalities in glutamate transporters and death of astroglia and neurons in newborn striatum. Ann Neurol. 42, 335-348   DOI   ScienceOn
30 McDonald, W., F. S. Silverstein and M. V. Johnston. 1988. Neurotoxicity of N-methyl-D-aspartate is markedly enhanced in developing rat central nervous system. Brain Res. 459, 200-203   DOI   ScienceOn
31 Gu, Y. Z., J. B. Hogenesch, C. A. Bradfield. The PAS superfamily: Sensors of environmental and developmental signals. Annu Rev. Pharmacol. Toxicol. 40, 519-561   DOI   ScienceOn
32 Halliwell, B., J. M. Gutteridge. 1985. The importance of free radicals and catalytic metal ions in human diseases. Mol. Aspects Med. 8, 89-193   DOI   ScienceOn
33 Hochachka, P.W., P. Lutz. 2001. Mechanism, origin, and evolution of anoxia tolerance in animals. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 130, 435-459   DOI   ScienceOn
34 Hsieh, M. T., W. H. Peng, C. R. Wu and W. H. Wang. 2000. The ameliorating effects of the cognitive-enhancing Chinese herbs on scopolamine-induced amnesia in rats. Phytother. Res. 14, 375-377   DOI   ScienceOn
35 Kerr, J. F. R., A. H. Wyllie and A. R. Currie. 1972. Apoptosis : a basic biological phenomenon with wideranging implications in tissue kinetics. Br. J. Cancer 26, 239-245   DOI
36 Jeong, H. W., S. Y. Kang, S. W. Bak. 1999. Effect of Rhizoma Acori Graminei Extract on Blood Pressure and Regional Cerebral Blood Flow in Rats. The Korea Association of Herbology 14, 81-88   과학기술학회마을
37 Jin, W., L. F. Qu, P. Min, S. Chen, H. Li, H. Lu and Y. T. Hou. 2004. Identification of genes responsive to apoptosis in HL-60 cells. Acta Pharmacol Sin. 25, 319-326
38 Kang, S. J., H. S. Keum, Y. Y. Jeon, E. J. Lee, C. S. Park, C. G. Park, J. H. Heo., C. H. Yang and J. S. Cho. 2001. The neuropropetective effect of Acori graminei rhizoma extract against cerebral ischemia in rats. Korean J. Orient.Int. Med. 22, 341-351   과학기술학회마을
39 Chauvet, C., B. Bois-Joyeux and J. L. Danan. 2002. Retinoic acid receptor-related orphan receptor(ROR) alpha4 is the predominant isoform of the nuclear receptor RORalpha in the liver and is up-regulated by hypoxia in HepG2 human hepatoma cells. Biochem J. 364, 449-456   DOI   ScienceOn
40 Cho, J., N. E. Joo, J. Y. Kong, D. Y. Jeong, K. D. Lee and B. S. Kang, 2000. Inhibition of excitotoxic neuronal death by methanol extract of Acori graminei rhizoma in cultured rat cortical neurons. J. Ethnopharmacol. 73, 31-37   DOI   ScienceOn
41 Cho, J., Y. H. Kim, J. Y. Kong, C. H. Yang and C. G. Park, 2002. Protection of cultured rat cortical neurons from excitotoxicity by asarone, a major essential oil component in the rhizomes of Acorus gramineus. Life Sci. 71, 591-599   DOI   ScienceOn
42 Colantuoni, C., A. E. Purcell, C. M. Bouton and J. Pevsner. 2000. High throughput analysis of gene expression in the human brain. J. Neurosci Res. 59, 1-10   DOI   ScienceOn
43 Fujio, Y., Y. Mitsuuchi, J. R. Testa and K. Walsh. 2001. Activation of Akt2 Inhibits anoikis and apoptosis induced by myogenic differentiation. Cell Death Differ. 8, 1207-1212   DOI   ScienceOn
44 Duggan, D. J., M. Bittner, Y. Chen, P. Meltzer and J. M. Trent. 1999. Expression profiling using cDNA microarrays. Nat Genet. 21, 10-14   DOI   ScienceOn
45 Ebert, B. L., J. D. Firth, P. J. Ratcliffe. 1995. Hypoxia and mitochondrial inhibitors regulate expression of glucose transporter-1 via distinct Cis-acting sequences. J. Biol Chem. 270, 29083-29089   DOI
46 Friedman, J. E. and G. G. Haddad. 1993. Major differences in $Ca^{2+}$, response to anoxia between neonatal and adult rat CA1 neurons: role of $Ca^{2+}$ and $Na^+$. J. Neurosci. 13, 63-72   DOI
47 Goldberg M. A., S. P. Dunning and H. F. Bunn. 1988. Regulation of the erythropoietin gene: evidence that the oxygen sensor is a heme protein. Science. 242, 1412-1415   DOI
48 Ashktorab, H., A. Ahmed, G. Littleton, X. W. Wang, C. R. Allen, R. Tackey, C. Walters and D. T. Smoot. 2003. p53 and p14 increase sensitivity of gastric cells to H. pylori-induced apoptosis, Dig Dis Sci. 48, 1284-1291   DOI   ScienceOn
49 Banasiak, K. J. and G. G. Haddad. 1998. Hypoxia-induced apoptosis: effect of hypoxic severity and role of p53 in neuronal cell death. Brain Res. 797, 295-304   DOI   ScienceOn
50 Barks, J.D. and F. S. Silverstein. 1992. Excitatory amino acids contribute to the pathogenesis of perinatal hypoxic-ischemic brain injury. Brain Pathol. 235-243
51 Beilharz, E. J. and C. E. Williams, M. Dragunow, E. S. Sirmanne and P. D. Gluckman. 1995. Mechanisms of delayed cell death following hypoxic-ischemic injury in the immature rat: evidence for apoptosis during selective neuronal loss. Mol Brain Res. 29, 1-14   DOI   ScienceOn
52 Chang, H. H. P. and P. H. But. 1986. In Pharmacology and Applications of Chinese Materia Medica vol. 1, pp. 334-337. World Scientific Publishing, Singapore
53 Besnard, S., C. Heymes, R. Merval, M. Rodriguez, J. P. Galizzi, J. A. Boutin, J. Mariani and A. Tedgui, 2002. Expression and regulation of the nuclear receptor RORalpha in human vascular cells. FEBS Lett. 511, 36-40   DOI   ScienceOn
54 Brewer, G. J., J. R. Torricelli, E. K. Evege and P. J. Price. 1993. Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination. J. Neurosci Res. 35, 567-676   DOI   ScienceOn
55 Brewster, J.L., S. L. Martin, J. Toms, D. Goss, K. Wang, K. Zachrone, A. Davis, G. Carlson, L. Hood and J. D. Coffin. 2000. Deletion of Dad1 in mice induces an apoptosis-associated embryonic death. Genesis 26, 271-278   DOI   ScienceOn
56 Chae, H. J., N. Ke, H. R. Kim, S. Chen, A. Codzik, M. Dickman and J. C. Reed. 2003. Evolutionarily conserved cytoprotection provided by Bax Inhibitor-1 homologs from animals, plants, and yeast. Gene 323, 101-113   DOI   ScienceOn
57 Nilsson, G. E. 2001. Surviving anoxia with the brain turned on. News Physiol Sci. 16, 217-221
58 Hansen, A. 1977. Extracellular potassium concentration in juvenile and adult brain cortex during anoxia. Acta. Physio.l Scand. 99, 412-420   DOI   ScienceOn