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http://dx.doi.org/10.5352/JLS.2007.17.12.1654

Effects of Single Total Body Irradiation (TBI) on the Peripheral Blood of Piglets  

Lee, Jae-Hoon (Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University)
Kang, Eun-Hee (Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University)
Chung, Dai-Jung (Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University)
Kim, Dong-Ku (College of Medicine, CHA Stem Cell Institute Pochon CHA University)
Kim, Jae-Hwan (Cell and Gene Therapy Research Institute, CHA Stem Cell Institute, Pochon CHA University)
Shin, Hyun-Soo (Department of Radiation Oncology, Bundang CHA Hospital, Pochon CHA University)
Ahn, Jung-Yong (Department of Neurosurgery, Yonsei Brain Research Institute, Yonsei University College of Medicine)
Park, Jin-Ki (Animal Biotechnology Division, National Institute of Animal Science)
Chang, Won-Kyong (Animal Biotechnology Division, National Institute of Animal Science)
Choi, Chi-Bong (Department of Biomedical Engineering, College of Medicine, The Catholic University of Korea)
Kim, Hwi-Yool (Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University)
Publication Information
Journal of Life Science / v.17, no.12, 2007 , pp. 1654-1659 More about this Journal
Abstract
We examined total body irradiation (TBI)-induced effects by complete blood count (CBC) and fluorescence-activated cell sorter analysis (FACS) in the piglet following radiation irritation. A CBC included red blood cell count, white blood cell count, and platelet cell count. Four piglets were examined in this study and each piglet was divided by irradiation dose, two piglets with 4 Gy, two with 6 Gy, one with 8 Gy. All piglets showed leukopenia, thrombocytopenia after irradiation. In 6 and 8 Gy group, three piglets showed severe hemostatic disorder and gastrointestinal disorder suchas diarrhea and anorexia, and they died between 10 and 15 days after radiation irritation. In 4 Gy, two piglets showed no clinical sign after radiation injury, but persistent leukopenia was shown in blood examination. We suggest that a single TBI dose less than 6 Gy is adequate for conditioning piglet for bone marrow transplantation.
Keywords
Total body irradiation; immunosuppression; FACS; piglets;
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