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http://dx.doi.org/10.5352/JLS.2007.17.11.1505

Investigation of Single Nucleotide Polymorphisms in the Adipocyte Fatty-Acid Binding Protein (FABP4) Gene  

Kim, Sang-Wook (Department of Animal Scinece, Pusan National University)
Jung, Ji-Hye (Department of Animal Scinece, Pusan National University)
Kim, Kwan-Suk (Department of Animal Science, Chungbuk National University)
Lee, Cheol-Koo (College of Life Sciences & Biotechnology, Korea University)
Kim, Jong-Joo (Department of Biotechnology, Yeungnam National University)
Choi, Bong-Hwan (Animal Genomics and Bioinformatics Division, National Livestock Research Institute)
Kim, Tae-Hun (Animal Genomics and Bioinformatics Division, National Livestock Research Institute, RDA)
Song, Ki-Duk (NICHD/National Institutes of Health)
Cho, Byung-Wook (Department of Animal Scinece, Pusan National University)
Publication Information
Journal of Life Science / v.17, no.11, 2007 , pp. 1505-1510 More about this Journal
Abstract
We found 8 single nucleotide polymorphisms (SNPs) in adipocyte fatty acid bonding protein (FABP4) gene as candidate gene of FAT1 locus on pig chromosome 4. With over 800 heads of major commercial pig breeds including Duroc, Landrace, Berkshire and Yorkshire, we analyzed SNPs of FABP4 gene to determine possible effects of FABP4 genotype to economically important traits. $400{\sim}800\;bp$ amplicons in FABP4 gene were used PCR-RFLP for each SNPs and we found that the frequency of some SNPs of this gene was different among the breeds. According to the statistical analyses to determine possible associations of each genotype with economic traits, it was found that subgroup with different genotypes showed significant differences in daily gain, backfat thickness, lean percentage and feed conversion ratio (P<0.05). Thus, as a Part of enhancing the selection competence related to swine growth rate and lean percentage, it is expected that FABP4 gene markers verified in this study will be useful to use for Korean commercial pig industry.
Keywords
Economic traits; swine; SNP Detection; PCR-RFLP; polymorphism; FABP;
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