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http://dx.doi.org/10.5352/JLS.2007.17.10.1374

Detection and Distribution of Bacterial Pathogens in Raw Water and During Water Treatment Process by Polymerase Chain Reaction  

Park, Hong-Ki (Water Quality Institute, Water Works HQ of Busan Metropolitan City)
Jung, Eun-Young (Water Quality Institute, Water Works HQ of Busan Metropolitan City)
Jung, Jong-Moon (Water Quality Institute, Water Works HQ of Busan Metropolitan City)
Yu, Pyung-Jong (Water Quality Institute, Water Works HQ of Busan Metropolitan City)
Publication Information
Journal of Life Science / v.17, no.10, 2007 , pp. 1374-1380 More about this Journal
Abstract
The development of polymerase chain reaction (PCR) technology has the potential to solve for isolating pathogenic microorganisms from environmental samples than traditional plate counting methods. We have been detected pathogenic bacteria from raw water and water treatment process in Busan metropolitan city by PCR method. According to the result of survey from July 2004 to October 2005, 80 out of 92(87.0%) were positive for bacterial pathogens in raw water samples and positive rate of Shigella spp., Yersinia spp., Salmonella spp. and Legionella spp. were 46.2%, 40.7%, 17.6% and 9.9%, respectively. Pathogenic bacteria in raw water was mainly distributed through the lately Autumn to the winter and more highly detected Maeri than Mulgum region. During the period of survey in water treatment process, Shigella spp. was highly detected but all of bacterial pathogens were entirely removed after in post-ozonation step. These suggest that waters supplied in Busan metropolitan city may be safe against the pathogenic bacteria.
Keywords
Pathogenic bacteria; post-ozonation step; polymerase chain reaction (PCR);
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1 Rou, D. C. 1997. Optimization of Water treatment process by Ozone and Granular Activated Carbon. Theme of Master in National of Bukyeng University.
2 Sobsey, M. D. 1989. Inactivation of health-realted microorganism in water by disinfection process. Wat. Sci. Tech. 21, 179-195.   DOI
3 Soumet, C., G. Ermel, N. Rose, V. Rose, P. Drouin, G. Salvat and P. Colin. 1999. Evaluation of a multiplex PCR assay for simultaneous identification of Salmonella sp., Salmonella Enteritidis and Salmonella Typhimurium for environmental swabs of poultry houses. Letters in Applied Microbiology 28, 113-117.   DOI   ScienceOn
4 Swapan, K. N. 2005. Shigellosis. The Journal of Microbiology 43, 133-143.   과학기술학회마을
5 Theron, J., D. Morar, M. D. U. Preez, V. S. Brozel and S. N. Venter. 2001. A sensitive seminested PCR method for the detection of shigella in spiked environmental water samples. Wat. Res. 35, 869-874.   DOI   ScienceOn
6 Waage, A. S., T. Vardund, V. Lund and G. Kapperud. 1999. Detection of low numbers of Salmonella in environmental water, sewage and food samples by a nested polymerase chain reaction assay. Journal of Applied Microbiology 87, 418-428.   DOI   ScienceOn
7 WHO. 1996. Guidelines for Drinking-Water Quality.
8 Xuanxian, P., L. Wen, Z. Jianying, W. Sanying and L. Shengcai. 2002. Rapid detection of Shigella species in environmental sewage by an immunocapture PCR with universal primers. Appl. Environ. Microbial. 68, 2580-2583.   DOI   ScienceOn
9 Kong, R. Y. C., Kong, S. K. Y. Lee, T. W. F. Law, S. H. W. Law and R. S. S. Wu. 2002. Rapid detection of six types of bacterial pathogens im marine waters by multiplex PCR. Water Research 36, 2802-2812.   DOI   ScienceOn
10 Ministry of Environment. 2002. Standard Method for the Drinking Water Quality.
11 National Institute of Environmental Research. 1998. Water Quality Management for Health and Treatment related microorganisms.
12 National Institute of Environmental Research. 1994. Development of Rapid Detection Method of Hazardous Materials in Water.
13 OECD. Eutrophication of waters. 1982. Monitoring, Assesment and Control, 154, Paris.
14 Park, H. K., C. M. Chung, J. R. Bahk and Y. K. Hong. 1999. The Relationship between Phytoplankton Productivity and Water Quality in Downstream of Nakdong River. J. of the Korean Environmental Sciences Society 8, 101-106.   과학기술학회마을
15 Park, H. K., E. Y. Jung, Y. J. Lee, J. M. Jung, D. H. Choi, H. J. Son, K. W. Kwon and Y. K. Hong. 2003. Distribution of Waterborne Enteric Viruses in Raw Water and Tap Water in Busan Metropolitan City. Kor. J. of Life Science 13, 197-205.   과학기술학회마을   DOI   ScienceOn
16 Park, J. Y. 1994. Drinking Water Microbiology. pp. 385-396, Chemical Engineering Research Corporation, Seoul.
17 Radnedge, L., S. Gamez, P. M. Mccready, P. L. Worsham and G. L. Andersen. 2002. Identification of nucleotide sequences for the specific and rapid detection of Yersinia pestis. Appl. Environ. Microbial. 67, 3759-3762.
18 Rose, J. B., C. N Haas and S. Regli. 1991. Risk assessment and control of waterborne giardiasis. American J. Public Health 81, 709-713.   DOI   ScienceOn
19 Roszak, D. B. and R. R. Colwell. 1987. Survival strategies of bacteria in the natural environment. Microbial Rev. 51, 365-379.
20 Baudart, J., K. Lemarchand, A. Brisabois and P. Lebaron. 2000. Diversity of salmonella strains isolated from the aqutic environment as determined by serotyping and amplification of the ribosomal DNA spacer regions. Appl. Environ. Microbial. 66, 1544-1552.   DOI   ScienceOn
21 Bej, A. K., M. H. Mahbubani and R. M. Atlas. 1991. Detection of viable Legionella pneumophila in water by Polymerase Chain Reaction and gene probe methods. Appl. Environ. Microbial. 57, 597-600.
22 Benenson, A. S. 1990. Control of Communicable Disease in Man, 15th. American Public Health Association.
23 Craun, G. F. 1986. Waterborne Disease in the United States. CRC Press, Boca Raton, FL.
24 Feachem, R., D. J. Bradley, H. Garelick and D. Mara. 1983. Sanitation and Disease : Health Aspects of Excreta and Wastewater Management. John Wiley and Sons, New York.
25 Fred, G. P., E. Lee and J. L. Anne. 1993. Public heath significance of waterborne pathogens in domestic water supplies and reclaimed water, Landfills and water qulity management.
26 Graham, D. Y., G. R. Dufour, and M. Estes, 1987. Minimal infective G. R. Dufour and M. Estes. dose of rotavirus. Arch. Virol. 92, 261-271.   DOI
27 Helbig, J. H., T. Engelstadter, M. Maiwald, S. A. Uldum, W. Witzleb and P. C. Luck. 1999. Diagnostic relevance of the detection of Legionella DNA in urine samples by the polymerase chain reaction. Eur. J. Clin. Microbiol. Infect Dis. 18, 716-722.   DOI   ScienceOn
28 Jung, H. M. and J. Y. Yoon. 1994. The Opinion with Microbiological Standard of American Drinking Water. J. KSWQ. 10, 62-71.
29 APHA, Standard methods for the examination of water and wastewater. 1992. 18th eds., APHA-AWWA-WPCF, New York.
30 Kapperud, G., T. Vardund, E. Skjerve, E. Hornes and T. E. Michaelsen. 1993. Detection of pathogenic Yersinia entericolitica in foods and water by immunomagnetic separation, nested polymerase chain reaction, and colorimetric detection of amplified DNA. Appl. Environ. Microbial. 59, 2938-2944.
31 Bach, H., S. Tarre and M. Green. 1998. Post treatment of groundwater denitrification fluidized bed reactor effluents to achieve drinking water quality. J. Industrial Microbiol. ＆ Biotechn. 20, 54-359.