Browse > Article
http://dx.doi.org/10.5352/JLS.2007.17.10.1354

Effect of Tea Polyphenols on Anticancer Activity and Cytokines Production  

Shon, Mi-Yae (Dept. of Food and Nutrition, Gyeongsang National University)
Nam, Sang-Hae (Dept. of Food Science, Jinju National University)
Publication Information
Journal of Life Science / v.17, no.10, 2007 , pp. 1354-1360 More about this Journal
Abstract
Theaflavins (TF) and thearubigins (TR) are constituents of tea pigments which are polyphenols derived from Korean fermentation tea. After TF, TR and [(-) epigallocatechin-3-gallate](EGCG) have been applied to macrophage cell line (RAW264.7) nitric oxide (NO) synthesis and cytokines production were estimated. Cytokines production by enzyme linked immune-sorbent assay (ELISA) determined. NO production was increased by about 1.5-folds at the dose of $80\;{\mu}g/ml$ compared to control and lipopolysaccharide (LPS) stimulation when TF, TR and EGCG were applied to a RAW264.7 cell. Interleukin-6 (IL-6), Tumor necrosis factor ($TNF-{\alpha}$) and granulocyte-macrophage colony stimulating factor (GM-CSF) increased depended on concentrations of TF, TR and EGCG. The production of tumor necrosis $factor-{\alpha}$ increased highly in TR, TF and EGCG group with LPS. These results suggest that TF, TR and EGCG have immune-enhancement effect through the cytokine production. TF, TR and EGCG inhibited cancer cell viability, the anticancer effect of these polyphenols may explain the anti-tumor promotion action and antioxidant activity of these tea constituents.
Keywords
Theaflavins (TF); thearubigins (TR); immunomodulator cytokine; anticancer activity; antioxidant activity;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Ozgen, M., R. N. Reese, A. Z. Tulio, J. C. Scheerens and A. R. Miller. 2006. Modified 2,2-azino-bis-3-ethylbenzothiazoline- 6-sulfonic acid (ABTS) method to measure antioxidant capacity of selected small fruits and comparison to ferric reducing antioxidant power (FRAP) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) methods. J. Agric. Food Chem. 54, 1151-1157.   DOI   ScienceOn
2 Shon, M. Y., S. K. Park and S. H. Nam. 2007. Antioxidant activity of theaflavin and thearubigin separated from Korean microbially fermented tea. J. Food Sci. Nutr. 12, 7-10.   과학기술학회마을   DOI   ScienceOn
3 Parronchi, P., D. Macchia, M. P. Piccinni, P. Biswas, C. Simonelli, E. Maggi, M. Ricci, A. A. Ansari and S. Romagnani. 1991. Allergen and bacterial antigen-specific T-cell clones established from atopic donors show a different profile of cytokine production. In: proceedings of the National Academy of Sciences of the United States of America 88, 4538-4542.   DOI   ScienceOn
4 Riemersma, R. A., C. A. Rice-Evans., R. M. Tyrrell., M. N. Clifford and M. E. Lean. 2001. Tea flavonoids and cardiovascular health. J. Med. 94, 277-282.
5 Roberts, E. A. H. and R. F. Smith. 1961. Spectrophotometer measurements of theaflavins and thearubigins in black tea liquors in assessments of quality in teas. The analyst 86, 94-98.   DOI
6 Schlesier, K., M. Harwat, V. Bohm and R. Bitsch. 2002. Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. J. Agric. Food Chem. 50, 81-86.   DOI   ScienceOn
7 Shiraki, M., Y. Hara, T. Osawa, H. Kumon, T. Nakauma and S. Kawaskishi. 1994. Antioxidative and antimutagenic effects of theaflavins from black tea. Mut. Res. 323, 29-34.   DOI   ScienceOn
8 Skehan, P., R. Storeng, D. Scudiero, A. Monks, L. McMahon, D. Vistica, T. Waren, H. Bokesch, S. Kenney and M. R. Boyd. 1990. New colorimetric cytotoxicity assay for anticancer drug screening. J. National Cancer Institute 82, 1107-1112.   DOI
9 Xie, B., H. Shi, Q. Chen and C. T. Ho. 1993. Antioxidant properties of fractions and polyphenol constituents from green, oolong and black teas. Proc. Natl. Sci. Council Rep. China. 17, 77-84.
10 Terao, J., M. Piskula and Q. Yao. 1994. Protective effects by epicatechin, epicatechin gallate and quercetin on lipid peroxidation in phospholipid bilayers. Arch. Biochem. Biophy. 308, 278-284.   DOI   ScienceOn
11 Yee, S. T., Y. R. Jeong, M. H. Ha, S. H. Kim, M. M. Byun and S. K. Jo. 2000. Induction of nitric oxide and TNF-$\alpha$ by herbal plant extract in mouse macrophage. J. Korean Soc. Food Sci. Nutr. 29, 342-348.
12 De la Puerta, R., M. E. M. Domingue, V. Rui-Gutierre, J. A. Flavill and J. R. S., Hoult. 2001. Effects of virgin olive oil phenolics on scavenging of reactive nitrogen species and upon nitrergic neurotransmission. Life Sci. 69, 1213-1222.   DOI   ScienceOn
13 Feihl, F., M. Oddo, B. Waeber, L. Liaudet. 2004. In hibitors of nitrogenoxide species production in animal models of inflammation and future directions for therapy in inflammatory disorders. Curr. Med. Chem. Anti-Inflammatory Anti-Allergy Agents 3, 239-259.   DOI   ScienceOn
14 Hibbs, J. B. Jr., R. R. Taintor, Z. Vavrin and E. M. Rachlin. 1980. Nitric oxide: a cytotoxic activated macrophage effector molecule. Biochem. Biophys. Res. Commun. 157, 87-94.   DOI   ScienceOn
15 Hilton P. J. and R. T. Ellis. 1972. Estimation of market value of Central African tea by theaflavin analysis. J. Agric. Food Chem. 50, 1833-1839.
16 Jeong, H. J. 2003. Immune-enhancement effect of the herbal combination Allergina. Clinica. Chimica. Acta. 337, 77-84.   DOI   ScienceOn
17 Nathan, C. F. 1987. Secretory products of macrophages. J. Clin. Invest. 79, 319-326.   DOI   ScienceOn
18 Keller, R. and R. Keist. 1989. Abilities of activated macrophages to manifest tumoricidal activity and to generate reactive nitrogen intermediates: a comparative study in vitro and ex vivo. Biochem. Biophys. Res. Commun. 157, 87-94.
19 Marcocci, L., J. J. Maguire, M. T. Droy-Lefaix and L. Packer. 1994. The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761. Biochem. Biophy. Res. Commun. 201, 748-755.   DOI   ScienceOn
20 Martin, O. P., O. Okinda, M. Richard and M. Mutuku. 2004. Changes in thearubigin fractions and theaflavin levels due to variations in processing conditions and their influence on black tea liquor brightness and total colour. Food Chem. 85, 163-173.   DOI   ScienceOn
21 Andras, H., C. Ildiko, B. Tamas and K. C. Laszlo. 2005. The cytotoxic antitumor effect of MTH-68/H, a live attenuated Newcastle disease virus is mediated by the induction of nitric oxide synthesis in rat peritoneal macrophages in vitro. Cancer Lett. 20, 1-11.
22 Brune, B. 2003. Nitric oxide: NO apoptosis or turning it ON? Cell Death Differ. 10, 864-869.   DOI   ScienceOn
23 Baugh, J. A. and R. Bucala. 2001. Mechanisms for modulating TNF alpha in immune and inflammatory disease. Curr. Opin. Drug. Discov. Devel. 4, 635-650.
24 Benzie, I. F. F. and J. J. Strain. 1996. The ferric reducing ability of plasma(FRAP) as measurement of 'antioxidant power': The Frap assay. Anal. Biochem. 239, 70-76.   DOI   ScienceOn
25 Brannellec, D. and S. Chouaib. 1991. TNF: antitumoral agent at the border lines of immunity and inflammation. Oathologie 39, 230-239.
26 Carter, L. L. and R. W. Dutton. 1996. Type 1 and type 2: a fundamental dichotomy for all T-cell subsets. Curr. Opi. In. Immu. 8, 336-342.   DOI   ScienceOn
27 Dalgleish, A. G. 2000. Cancer vaccines. British J. Cancer 82, 1619-1624.   DOI   ScienceOn