Browse > Article
http://dx.doi.org/10.5352/JLS.2006.16.7.1229

Up-regulation of Matrix Metalloproteinase-9 in Smooth Muscle Cell Undergoing Death  

Lee, Kyeong-Ah (Department of Pharmacology, School of Medicine, Pusan National University)
Kim, Sun-Mi (Department of Pharmacology, School of Medicine, Pusan National University)
Kim, Koan-Hoi (Department of Pharmacology, School of Medicine, Pusan National University)
Publication Information
Journal of Life Science / v.16, no.7, 2006 , pp. 1229-1234 More about this Journal
Abstract
This study investigated whether matrix metalloproteinases (MMPs) can be modified in apoptotic smooth muscle cell (SMC) using the SMC that undergoes apoptotic death by expressing Fas-associated death domain containing protein (FADD) when they are grown without tetracycline in culture medium. In the absence of tetracycline, FADD-SMC lost adherence and showed the fragmentation of the nuclei. In proportion to duration of tetracycline removal, phosphorylated form of p38 MAPK and of ERK increased, whereas phosphorylation of protein kinase B (PKB) was not changed very much in response to tetracycline The levels of cyclin A and cyclin D were also decreased in a time dependent manner. Up-regulation of MMP-9 expression and activity was observed when the SMC were grown without tetracycline. Immunoreactivity of MMP-9 was detected from both attached and floating FADD-SMCs grown without tetracycline. An inhibitor of MAPK kinase, PD098059, and an inhibitor of p38 MAPK, SB203580, inhibited the up-regulation of MMP-9. Treatment of the SMC with a synthetic MMP inhibitor, BB94, attenuated death occurring in the absence of tetracycline. These results indicate that SMC undergoing death is able to up-regulate MMP-9 and that the enzyme can affect cell viability.
Keywords
Metalloproteinase; MAPK; smooth muscle cell;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Nguyen M, J Arkell, CJ Jackson. 1998. Active and tissue inhibitor of matrix metalloproteinase-free gelatinase B accumulates within human microvascular endothelial vesicles. J. Biol. Chem. 273, 5400-4   DOI   ScienceOn
2 Ram M, Y Sherer, Y Shoenfeld. 2006. Matrix metalloproteinase-9 and autoimmune diseases. J. Clin. Immunol. 26, 299-307   DOI   ScienceOn
3 Schonbeck U, F Mach, GK Sukhova, C Murphy, JY Bonnefoy, RP Fabunmi, P Libby. 1997. Regulation of matrix metalloproteinase expression in human vascular smooth muscle cells by T lymphocytes:a role for CD40 signaling in plaque rupture? Circ. Res. 81, 448-54   DOI   ScienceOn
4 Simon C, H Goepfert, D Boyd. 1998. Inhibition of the p38 mitogen-activated protein kinase by SB 203580 blocks PMA-induced Mr 92,000 type IV collagenase secretion and in vitro invasion. Cancer Res. 58, 1135-9
5 Sinha S, WH Frishman. 1998. Matrix metalloproteinases and abdominal aortic aneurysms: a potential therapeutic target. J. Clin. Pharmacol. 38, 1077-88
6 Bauriedel G, R Hutter, U Welsch, R Bach, H Sievert, B Luderitz. 1999. Role of smooth muscle cell death in advanced coronary primary lesions: implications for plaque instability. Cardiovasc. Res. 41, 480-8   DOI   ScienceOn
7 Loftus IM, S Goodall, M Crowther, L Jones, PR Bell, AR Naylor, 1999. Thompson MM. Increased MMP-9 activity in acute carotid plaques: therapeutic avenues to prevent stroke. Ann. N. Y. Acad. Sci. 878, 551-4   DOI   ScienceOn
8 Vaillant C, C Meissirel, M Mutin, MF Belin, LR Lund, N Thomasset. 2003. MMP-9 deficiency affects axonal outgrowth, migration, and apoptosis in the developing cerebellum. Mol. Cell. Neurosci. 24, 395-408   DOI   ScienceOn
9 Simon C, MJ Hicks, AJ Nemechek, R Mehta, Jr. O'MalleyBW, H Goepfert, CM Flaitz, D Boyd. 1999. PD 098059, an inhibitor of ERK1 activation, attenuates the in vivo invasiveness of head and neck squamous cell carcinoma. Br. J. Cancer 80, 1412-9   DOI   ScienceOn
10 Sukhova GK, U Schonbeck., E Rabkin, FJ Schoen, AR Poole, RC Billinghurst, P Libby. 1999. Evidence for increased collagenolysis by interstitial collagenases-1 and -3 in vulnerable human atheromatous plaques. Circulation 99, 2503-9   DOI   ScienceOn
11 Galis ZS, M Muszynski, GK Sukhova, E Simon-Morrissey, P Libby. 1995. Enhanced expression of vascular matrix metalloproteinases induced in vitro by cytokines and in regions of human atherosclerotic lesions. Ann. N. Y. Acad. Sci. 748, 501-7
12 Galis ZS, GK Sukhova, MW Lark, P Libby. 1994. Increased expression of matrix metalloproteinases and matrix degrading activity in vulnerable regions of human atherosclerotic plaques. J. Clin. Invest. 94, 2493-503   DOI   ScienceOn
13 Gum R, H Wang, E Lengyel, J Juarez, D Boyd. 1997. Regulation of 92 kDa type IV collagenase expression by the jun aminoterminal kinase- and the extracellular signal-regulated kinase-dependent signaling cascades. Oncogene 14, 1481-93   DOI   ScienceOn
14 Mandal M, A Mandal, S Das, T Chakraborti, c Sajal. 2003. Clinical implications of matrix metalloproteinases. Mol. Cell. Biochem. 252, 305-29   DOI   ScienceOn
15 Knox JB, GK Sukhova, AD Whittemore, P Libby. 1997. Evidence for altered balance between matrix metalloproteinases and their inhibitors in human aortic diseases. Circulation 95, 205-12   DOI   ScienceOn
16 Kockx MM, AG Herman. 2000. Apoptosis in atherosclerosis:beneficial or detrimental? Cardiovasc. Res. 45, 736-46   DOI   ScienceOn
17 Libby P, M Aikawa. 2002. Stabilization of atherosclerotic plaques: new mechanisms and clinical targets. Nat. Med. 8, 1257-62   DOI   ScienceOn
18 McCawley LJ, LM Matrisian. 2001. Matrix metalloproteinases:they're not just for matrix anymore! Curr. Opin. Cell Biol. 13, 534-40   DOI   ScienceOn
19 Mott JD, Z Werb. 2004. Regulation of matrix biology by matrix metalloproteinases. Curr. Opin. Cell. Biol. 16, 558-64   DOI   ScienceOn
20 Alexander CM, EW. Howard, MJ. Bissell, Z. Werb. 1996. Rescue of mammary epithelial cell apoptosis and entactin degradation by a tissue inhibitor of metalloproteinases-1 transgene. J. Cell Biol. 135, 1669-77   DOI   ScienceOn
21 Boudreau N, CJ Sympson, Z Werb, MJ Bissell. 1995. Suppression of ICE and apoptosis in mammary epithelial cells by extracellular matrix. Science 267, 891-3   DOI   ScienceOn
22 Chakraborti S, M Marldal, S Das, A Mandal, T Chakraborti. 2003. Regulation of matrix metalloproteinases: an overview. Mol. Cell. Biochem. 253, 269-85   DOI   ScienceOn
23 Chintala SK, X Zhang, JS Austin, ME Fini. 2002. Deficiency in matrix metalloproteinase gelatinase B (MMP-9) protects against retinal ganglion cell death after optic nerve ligation. J. BioI. Chem. 277, 47461-8   DOI   ScienceOn
24 Cho A, J Graves, MA Reidy. 2000. Mitogen-activated protein kinases mediate matrix metalloproteinase-9 expression in vascular smooth muscle cells. Arterioscler. Thromb. Vasc. Biol. 20, 2527-32   DOI   ScienceOn
25 Galis ZS, JJ Khatri. 2002. Matrix metalloproteinases in vascular remodeling and atherogenesis: the good, the bad, and the ugly. Circ. Res. 90, 251-62