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http://dx.doi.org/10.5352/JLS.2006.16.7.1207

Tumor Necrosis factor-α Promotes Osteogenesis of Human Bone Marrow-derived Mesenchymal Stem Cells through JNK-dependent Pathway  

Kim, Mi-Ra (Medical Research Center for Ischemic Tissue Regeneration & Medical Research Institute)
Song, Hae-Young (Medical Research Center for Ischemic Tissue Regeneration & Medical Research Institute)
Kim, Jae-Ho (Medical Research Center for Ischemic Tissue Regeneration & Medical Research Institute)
Publication Information
Journal of Life Science / v.16, no.7, 2006 , pp. 1207-1213 More about this Journal
Abstract
Tumor necrosis $factor-{\alpha}\;(TNF-{\alpha})$ has been implicated in skeletal diseases by promoting bone loss in inflammatory bone diseases. In the present study, we examined the effects of $TNF-{\alpha}$ on osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs). $TNF-{\alpha}$ dose-dependently promoted matrix mineralization of hBMSCs with a maximal stimulation at 2ng/ml. $TNF-{\alpha}$ increased expression of alkaline phosphatase, which plays a crucial role for the matrix deposition. The $TNF-{\alpha}-stimulated$ osteoblastic differentiation was not affected by $NF_kB$ inhibitors, BAY and SN50. However, a JNK-specific inhibitor, SP600125 completely abolished the $TNF-{\alpha}-stimulated$ matrix mineralization and expression of alkaline phosphatase. These results suggest that $TNF-{\alpha}$ enhances osteoblastic differentiation of hBMSCs through JNK-dependent pathway.
Keywords
Mesenchymal stem cells; TNF-alpha; differentiation; JNK; bone;
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