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http://dx.doi.org/10.5352/JLS.2006.16.6.925

Effects of ENA-A(ENA actimineral resource A) Ion Water on the Activity and Differentiation of MC3T3-E1 Osteoblastic cell  

Lee, Ji-Won (Dept. of Food Science & Technology, Keimyung University)
Jeon, Sang-Kyung (Dept. of Food Science & Technology, Keimyung University)
Kim, Hyun-Jeong (The Center for Traditional Microorganism Resources Center, Keimyung University)
Lee, In-Seon (The Center for Traditional Microorganism Resources Center, Keimyung University)
Publication Information
Journal of Life Science / v.16, no.6, 2006 , pp. 925-931 More about this Journal
Abstract
Culture of osteoblast is extremely valuable in analyzing biological features that are specific to bone. ENA-A, ENA actimineral resource A, is a seaweed origin alkaline water. To investigate the bioactivity of ENA which act on bone metabolism, we studied the effects of a ENA on the activity of osteoblast MC3T3-E1 cells. ENA (1, 2, 4%) dose-dependently increased survival (p<0.05) and alkaline phosphatase activity (p<0.05) on MC3T3-E1 cell. And examined histochemistry and nodule formation according to the time course. To determine the expression patterns of bone-related proteins during the MC3T3-E1 osteoblast-like cell differentiation by using RT-PCR. This study suggest that ENA may promote the function of osteoblastic cells and play an important role in bone formation.
Keywords
ENA; osteoblast; bone formation; proliferation; alkaline phosphatase; mineralization;
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