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http://dx.doi.org/10.5352/JLS.2006.16.6.919

Toxicity Evaluation of Endocrine Disrupting Chemicals Using Human HepG2 Cell Line, Lumbricus rubellus and Saccharomyces cerevisiae  

Sohn, Ho-Yong (Dept. of Food and Nutrition, Andong National University)
Kim, Hong-Ju (Dept. of Food and Nutrition, Andong National University)
Kum, Eun-Joo (Dept. of Food and Nutrition, Andong National University)
Cho, Min-Seop (The School of Bioresource Sciences, Andong National University)
Lee, Jung-Bok (The School of Bioresource Sciences, Andong National University)
Kim, Jong-Sik (Dept. of Biological Science, Andong National University)
Kwon, Gi-Seok (The School of Bioresource Sciences, Andong National University)
Publication Information
Journal of Life Science / v.16, no.6, 2006 , pp. 919-924 More about this Journal
Abstract
Toxicity evaluation systems for various chemicals and their metabolites have been developed during last decades. In this study, the acute toxicity of endocrine disrupting chemicals, such as endosulfan, bisphenol A, vinclozolin, and 3,5-dichloroaniline, was evaluated using HepG2 cell line, Lumbricus rubellus and Saccharomyces cerevisiae, respectively. The extents of toxicity of the chemicals in different bioassay systems varied substantially, such as endosulfan>3,5-dichloroaniline> bisphenol A in HepG2 cell line system, endosulfan>bisphenol A>3,5-dichloro aniline in L. rubellus system, and 3,5-dichloroaniline>endosulfan>bisphenol A in S. cerevisiae system. Meanwhile, no cytotoxicity was observed by treatment of vinclozolin in the evaluation systems. Our results suggest that earthworm and yeast are useful to evaluate acute toxicity of endocrine disrupting chemicals, and direct comparison of toxicity data from different bioassay systems is unattainable. Based on our results, we propose that the bioassay system with earthworm or yeast, a rapid, simple and economic system, could be applied as pre-test for the toxicity evaluation using human cell line or animals.
Keywords
Endocrine disrupting chemicals; HepG2 cell line; Lumbricus rubellus; Saccharomyces cerevisiae; toxicity evaluation;
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