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http://dx.doi.org/10.5352/JLS.2006.16.4.664

Molecular Typing of Salmonella enterica serovar Typhi Strains Isolated in Busan by Pulsed-Field Gel Electrophoresis  

Min, Sang-Kee (Division of Microbiology, Busan Institute Of Health and Environment)
Lee, Ju-Hyun (Division of Microbiology, Busan Institute Of Health and Environment)
Park, Eun-Hee (Division of Microbiology, Busan Institute Of Health and Environment)
Kim, Jung-Ah (Division of Microbiology, Busan Institute Of Health and Environment)
Kim, Kyu-Won (College of Pharmacy, Seoul National University)
Publication Information
Journal of Life Science / v.16, no.4, 2006 , pp. 664-671 More about this Journal
Abstract
We obtained 424 Salmonella enterica serovar Typhi isolates from sporadic cases of infection in Busan during 1996 to 2005. We investigated the trend of antimicrobial resistance and molecular typing by pulsed-field gel electrophoresis (PFGE). Of the total 424 isolates, 6 strains (1.4%) were multidrug-resistant (MDR) S. enterica serovar Typhi isolates, 2 strains (0.5%) were resistant to only nalidixic acid, and the remaining 416 strains (98.1%) were fully susceptible to the 18 antimicrobial agent. PFGE of XbaI-digested chromosomal DNA was performed on 50 sporadic S. enterica serovar Typhi isolates with the objective of investigating the extent of genetic diversity of these isolates in our region. We could find that these isolates were much more heterogeneous and at least 32 different PFGE patterns were generated according by dice coefficient, between 0.69 and 1.0. Restriction fragment patterns consisted of 13 to 18 fragments ranged in size from 20 to 630 kb. The results confirmed that PFGE would be an useful tool for investigating surveillance of sporadic or outbreak case and assessing clonality for S. enterica serovar Typhi in Busan area. Our finding will be valuable in developing rational strategies to control this pathogen and setting the basis of an effective PulseNet system in Korea.
Keywords
Salmonella enterica serovar Typhi; antimicrobial susceptibility; MDR; PFGE;
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