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http://dx.doi.org/10.5352/JLS.2006.16.4.566

A Potential Demerit of the Pronuclear Microinjection Technique  

Wang, Ai-Guo (Laboratory of Human Genomics, Genetic Resources Center)
Kim, Sun-Uk (Laboratory of Human Genomics, Genetic Resources Center)
Moon, Hyung-Bae (Department of Pathology, School of Medicine and Institute of Medical Science, Wonkwang University)
Hyun, Byung-Hwa (Korea National Primate Research Center)
Nam, Ki-Hoan (Lab of Animal Model Evaluation, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
Suh, Jun-Gyo (Experimental Animal Center, College of Medicine, Hallym University)
Kim, Nam-Soon (Laboratory of Human Genomics, Genetic Resources Center)
Yu, Dae-Yeul (Laboratory of Human Genomics, Genetic Resources Center)
Lee, Dong-Seok (Laboratory of Human Genomics, Genetic Resources Center)
Publication Information
Journal of Life Science / v.16, no.4, 2006 , pp. 566-570 More about this Journal
Abstract
Pronuclear microinjection (PMI) is a primary method for producing transgenic mice and offers a powerful tool for investigating gene function in vivo. The method has several reported advantages and disadvantages. Here, we report another potential shortcoming. The survival rate of fertilized one cell-stage embryos was significantly reduced after PMI procedure (65.4% (1202/1838)). In addition, the proportion of embryos developing to full-term was also significantly lower than that of embryos not undergoing PMI (26.5% (319/1202) vs 41.9% (57/136)). Moreover, 3 out of 21 (14.3%) founder control mice which were non-transgene-carrying littermates of transgenic founders showed histopathological changes in their liver, which was comparable to that in of transgenic lineages (4 out of 27 (14.8%)). In conclusion, the mechanical damages in chromosomes occurring during PMI procedure may be a potential factor influencing phenotypes of transgenic mice.
Keywords
Nuclear damage; pronuclear microinjection; transgenic mice;
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