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http://dx.doi.org/10.5352/JLS.2006.16.3.459

Induction of Shoot Primordium in Culture of Garlic (Allium sativum L.)  

Choi Joo-Soo (Department of Molecular Biology, Dong-Eui University)
Lee Bok-Kyu (Department of Molecular Biology, Dong-Eui University)
Huh Man-Kyu (Department of Molecular Biology, Dong-Eui University)
Publication Information
Journal of Life Science / v.16, no.3, 2006 , pp. 459-463 More about this Journal
Abstract
Cultivated garlic, Allium sativum L. is economically important for leaves and bulbs, which historically were used in Korea for spices and condiments of Korean food as well as medicine crops. This experiment was carried out to investigate the effect of development and differentiation on culture of A. sativum (cv: white 6) by explant position, hormone composition and sucrose concentration in culture media. Culture method was investigated to induce shoot primordium. Culture efficiency was better with lower tissue of foliage leaf in explant position and on the medium with NAA 0.02 + BAP 1.0 mg/l in hormone composition than any other. Precocious shoot and callus were induced from shoot apex. Shoot was efficiently differentiated on 4,000 mg/l sucrose with increasing concentration of BAP. Shoot primordium was also induced with liquid rotary culture by histological observation. Rhizoid was induced from callus tissue cluster on medium with NAA 0.02 + BAP 2.0 mg/l.
Keywords
Garlic (Allium sativum L.); liquid rotary culture; shoot primordium;
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1 Park, C. H., M. S. Lee, D. C. Choi and H. C. Lim. 1993. Effect of 1-Naphthaleneacetic acid and sucrose on bulblet formation in floral and vegetative bud culture of garlic (Allium sativum L.). J. Kor. Soc. Hort. Sci. 34, 248-256
2 Suh, S. K. and H. G. Park. 1995. Plant regeneration from the culture of garlic root explants. J. Kor. Soc. Hort. Sci. 36, 31-37
3 Tanaka, R. and H. Ikeda. 1983. Perennial maintenance of annual Haplppappus gracilis (2n=4) by shoot tip cloning. Japan. J. Genet. 58, 65-70
4 Tanaka, R., H. Ikeda, K. Taniguchi and A. Watanabe. 1983. Maintenance and Mass- propagation of an aneuploid in annual Haplopappus gracilis (2n=4) by shoot tip cloning. Proc. Japan. Acad. 59, 359-362
5 Tanaka, R., K. Taniguchi and I. Fujishige. 1988. Artificial induction of clonal tetraploids in an annual plant, Haplopappus gracilis (2n=4), by using tissue culture shoot primordia. Japan J. Genet. 63, 113-125   DOI
6 Tanaka, R., K. Taniguchi and Y. Kamisugi. 1985. Induction of single cell stage in tissue cultured shoot primordia of Haplopappus gracilis (2n=4), a preliminary report. Japan J. Genet. 60, 405-410   DOI
7 Walkey, B. G. A., M. J. W. Webb, C. J. boll and A. Miller. 1978. Production of virus free garlic (Allium sativum L.) and shallot (A. ascalonicum L.) by meristem tip culture. J. Hort. Sci. 62, 211-220
8 Choi, S. Y., K. Y. Paek and J. T. Jo. 1993. Plantlet production through callus culture in Allium sativum L. J. Kor. Soc. Hort. 34, 16-28
9 Choi, J. S. 1994. Mass propagation by shoot apex culture through induction of shoot primordia in some fruit trees. 1. Shoot apex culture of Apple trees. J. Inst. Bio-Prod. Res., Dongeui Univ. 11, 65-72
10 Choi, J. S. and M. K. Huh. 2004. Micropropagation by leaf blade culture through induction of callus and shoot primordium in Opiopogon japonicus Ker-Gawl. Dongeui Nonjip. 40, 235-242
11 Chung, H. D. and M. U. Chang. 1979. Studies on the infection of virus in garlic, Allium sativum L. in Korea. J. Kor. Soc. Hort. 20, 123-133
12 Lee, E. M. and Y. B. Lee. 1994a. Systematic propagation of high quality garlic (Allium sativum L.) through shoot apical meristem culture. 1. Organogenesis from in vitro cultured shoot tip. Korean J. Plant Tissue Culture 21, 161-166   과학기술학회마을
13 Nagai, T., Y. Nomura and K. Oosawa. 1989. Induction of shoot primordia and plantlet formation in melon. J. Japan. Soc. Hort. Sci. Abs. 58, 208-209
14 Okada, M. and M. Matsumoto. 1990. Shoot primordia induction and plant vegeneration in Allium chinense. J. Japan Soc. Hort. Sci. Abs. 59, 284-285
15 Suh, S. K. and H. G. Park. 1993. Rapid multiplication through immature bulbil cultures of garlic. J. Kor. Soc. Hort. Sci. 34, 173-178
16 Yang, S. G., H. S. Lee, W. J. Jeong, S. R. Min and J. R. Liu. 1993. Production of virus-free microbulbs of garlic (Allium sativum L.) by in vitro culture of vegetative and floral buds in immature involucre. J. Kor. Soc. Hort. Sci. 34, 179-183
17 Bojwani, S. S., D. Cohen and P. R. Fry. 1982. Production of virus free garlic and field performance of micropropagated plants. Scientia Hort. 18, 39-43   DOI   ScienceOn
18 Novak, F. J., L. Havel and J. Dolezel. 1986. Handbook of Plant Cell culture, Chapter 15; Allium. pp. 418-456, V4, Y.P.S Bajai, ed, Springer-Verlag, New York
19 Lee, E. M. and Y. B. Lee. 1994b. Systematic propagation of high quality garlic (Allium sativum L.) through shoot apical meristem culture. 2. Effect of sucrose concentration and nitrogen source on in vitro formation of bulblets. Korean J. Plant Tissue Culture 21, 193-199   과학기술학회마을
20 Vander Valk P., O. E. Scholten, F. Verstappen, R. C. Jansen and J. J. M. Dons. 1992. High frequency somatic embryogenesis and plant regeneration zygotic embryoderived callus of three Allium species. Plant Cell, Tissue and Organ Culture 30, 181-191   DOI
21 Taniguchi, K., R. Tanaka, N. Ashitani and H. Miyagawa. 1988. Freeze preservation of tissue-cultured. Shoot primordia of the annual Haplopappus gracilis(2n=4). Japan J. Genet. 63, 267-272   DOI