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http://dx.doi.org/10.5352/JLS.2006.16.3.375

Selective Reduction by Microbial Aldehyde Reductase  

Lee Young-Soo (Department of Chemistry, Myong Ji University)
Kim Kyung-Soon (Department of Chemistry, Myong Ji University)
Publication Information
Journal of Life Science / v.16, no.3, 2006 , pp. 375-381 More about this Journal
Abstract
Aldehyde reductase was purified to electrophoretic homogeneity from Saccharomyces cerevisiae, and then enzymatic reduction of substituted carbonyl compounds was carried out by using the purified aldehyde reductase as a biocatalyst. Under preparative scale reaction renditions, the enzymatic reduction proceeded in high chemical yield with excellent chemoselectivity. The enzymatic reduction product was identified by TLC, GC, Mass, NMR and FT-IR. Benzoic acid, an inhibitor of aldehyde reductase, also potently inhibited the reduction of substituded carbonyl compounds. This enzyme exhibited a broad substrate specificity , and can utilize both NADH and NADPH as cofactors. The enzyme was strongly inhibited by benzoic acid and quercetin. The apparent Km for 4-cyanobenzaldehyde and 3-nitrobenzamide were 4.894 mM and 0.305 mM, respectively.
Keywords
Selective reduction; reductase;
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