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http://dx.doi.org/10.5352/JLS.2006.16.2.282

Antitumor Sterol Isolated from the Fruiting Body of Pleurotus eryngii  

Lee Young-Hoon (Department of Applied Life Science, Gyeongsang National University)
Park Ki-Hun (Department of Microbiological Engineering, Chinju National University)
Lee Byong-Won (Department of Microbiological Engineering, Chinju National University)
Cho Yong-Un (Department of Applied Life Science, Gyeongsang National University)
Choi Young-Ju (Department of Food and Nutrition, Silla University)
Gal Sang-Wan (Department of Applied Life Science, Gyeongsang National University)
Publication Information
Journal of Life Science / v.16, no.2, 2006 , pp. 282-288 More about this Journal
Abstract
Activity-guided fractionations led to the isolation of antitumor compound, ergosterol peroxide ($5{\alpha},\;8{\alpha}-epideoxy-24(R)-methylcholesta-6,\;22-dien-3{\beta}-ol$) from the fruiting body of Pleuratus eryngii that was cultivated artificially. This sterol structure was established by using spectroscopic methods ($^1H\;and\;^{13}C$ nuclear magnetic resonance and high resolution mass spectra). The purified compound showed a molecular formular of $C_{28}H_{44}O_3$ displaying characteristic features of epidioxy sterols. The 50% inhibitory concentrations ($IC_{50}$) of ergosterol peroxide against human lung cancer cell line (A549) and human ovarian cell line (SK-OV3) were $7{\mu}M\;and\;14{\mu}M$, respectively. In the DNA fragmentation assay, the compound showed the programmed cell death causing the chromosomal DNA fragmentation. It reveals that ergosterol peroxide arrests G1 phase of the cell division cycle.
Keywords
Pleurotus eryngii; ergosterol peroxide; 50% inhibitory concentration; A549; SK-OV3;
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