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http://dx.doi.org/10.5352/JLS.2006.16.2.231

Gene Expression Profile Associated with the Differentiation of Osteoblasts from Human Mesenchymal Stem Cells  

Kim Yeo-Kyeoung (Department of Hematology/Oncology, Chonnam National University Medical School)
Kim Hee-Nam (Genome Research Center for Hematopoietic Diseases, Chonnam National University Hospital)
Lee Il-Kwon (Genome Research Center for Hematopoietic Diseases, Chonnam National University Hospital)
Park Kyeong-Soo (Genome Research Center for Hematopoietic Diseases, Chonnam National University Hospital)
Yang Deok-Hwan (Department of Hematology/Oncology, Chonnam National University Medical School)
Cho Sang-Hee (Department of Hematology/Oncology, Chonnam National University Medical School)
Lee Je-Jung (Department of Hematology/Oncology, Chonnam National University Medical School)
Chung Ik-Joo (Department of Hematology/Oncology, Chonnam National University Medical School)
Kim Soon-Hag (Department of Nuclear Medicine, Seoul National University College of Medicine)
Kim Hyeoung-Joon (Department of Hematology/Oncology, Chonnam National University Medical School, Genome Research Center for Hematopoietic Diseases, Chonnam National University Hospital)
Publication Information
Journal of Life Science / v.16, no.2, 2006 , pp. 231-239 More about this Journal
Abstract
Human mesenchymal stem cells (hMSCs) in bone marrow (BM) can be induced to differentiate into a variety of mesenchymal tissues, including adipocytes, osteoblasts and chondroblasts, under the influence of certain growth or environmental factors. In this study, we analyzed the differentiation process and the associated gene expression profiles inherent to the process by which hMSCs differentiate into osteoblasts. We conducted a comparison of gene expression profiles of the normal human BM MSCs, using human 8K cDNA microarray, incubated in media containing either a combination of $\beta$-glycerol phosphate, L-ascorbic acid, and dexamethasone, or in medium lacking these osteogenic supplements. During the osteoblastic differentiation process, 36 genes were determined to be up-regulated, and 59 genes were shown to be down-regulated. Osteoprotegerin, LRP5, and metallothionein 2A, all of which are associated with the osteogenetic process, were up-regulated, and genes associated with the differentiation of MSCs into other lineages, including muscle, adipose tissue and vascular structure were down-regulated. The set of differentially expressed genes reported in this work should contribute to our current understanding of the processes inherent to the differentiation of MSCs into osteoblasts.
Keywords
Gene expression; mesenchymal stem cell; osteoblast;
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