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http://dx.doi.org/10.5352/JLS.2006.16.2.186

Effects of Rhynchosia molubilis Saponin on Hepatotoxicity and Pathology  

Ha Bae-Jin (Department of Bioscience and Biotechnology, Silla University)
Publication Information
Journal of Life Science / v.16, no.2, 2006 , pp. 186-191 More about this Journal
Abstract
This study was carried out to investigate the effects of Rhynchosia molubilis saponin on carbon tetrachloride ($CCl_4$)-induced hepatotoxicity. Sprague-Dawley rats were intraperitoneally administered the Rhynchosia molubilis saponin at 100 mg/kg every day for two weeks, then $CCl_4$ (3.3 ml/kg) was injected into rats. 12 hours later, they were anesthesized with ether and dissected. Rhynchosia molubilis saponin-administered group showed 59.92% and 62.28% of inhibitory effects on aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities compared to $CCl_4$-treated group (p<0.05). Malonedialdehyde (MDA) levels of Rhynchosia molubilis Saponin-administered and $CCl_4$-treated (RSC) group in liver homogenate and mitochondria were significantly inhibited to 61.83%, 81.11 %, respectively, compared to $CCl_4$-treated group (p<0.05). Superoxide dismutase (SOD) activities of RSC group in liver homogenate and mitochondria were significantly inhibited to 66.53%, 31.04%, respectively, compared to $CCl_4$-treated group (p<0.05). GPx activities of RSC group in liver homogenate and mitochondria were significantly inhibited to 72.74%, 72.68%, respectively, compared to $CCl_4$-treated group (p<0.05). The histological examinations showed that the liver cell necrosis and centrilobular congestion aggregation induced by $CCl_4$ were dearly eliminated by the administration of Rhynchosia molubilis saponin. These results suggest that Rhynchosia molubilis saponin could have the protective effects against hepatotoxicity.
Keywords
Rhynchosia molubilis saponin; $CCl_4$; antioxidant enzyme; lipid peroxidation;
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