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http://dx.doi.org/10.5352/JLS.2006.16.1.076

In vivo Functional Analysis of γ-butyrolactone Autoregulator Receptor Gene (scaR) in Streptomyces clavuligerus  

Kang Su-Jin (Division of Food Science and Biotechnology, Kyungnam University)
Lee Chang-Kwon (Department of Physiology, College of Medicine, Institute of Biomedical Science and Technology, Konkuk University)
Choi Sun-Uk (Division of Food Science and Biotechnology, Kyungnam University)
Kim Hyun-Soo (Department of Microbiology, Keimyung University)
Hwang Yong-Il (Division of Food Science and Biotechnology, Kyungnam University)
Publication Information
Journal of Life Science / v.16, no.1, 2006 , pp. 76-81 More about this Journal
Abstract
A $\gamma-butyrolactone$ autoregulator receptor has a common activity as DNA-binding transcriptional repressors controlling secondary metabolism and/or morphological differentiation in Streptomyces. A gene (scaR) encoding it was cloned from Streptomyces cravuligerus, a clavulanic acid producer, and was in vitro characterized in a previous report. In this study to clarify the in vivo function of ScaR, a $\gamma-butyrolactone$ autoregulator receptor of Streptomyces clavuligerus, we constructed a scaR-deleted strain by means of homologous recombination. No difference in morphology was found between the wild-type strain and the scaR-disruptant, but the scaR-disruptant showed higher clavulanic acid production. This indicates that the ScaR in S. clavuligerus acts as a negative regulator of the biosynthesis of clavulanic acid, but plays no role in morphological differentiation.
Keywords
Streptomyces clavuligerus; clavulanic acid; autoregulator receptor; negative regulator;
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