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http://dx.doi.org/10.5352/JLS.2005.15.6.923

Development of Bactericidal Yeast Strain by Expressing the Leucocin A Gene on the Cell Surface of Saccharomyces cerevisiae  

Lee Sang-Hyeon (Department of Bioscience and Biotechnology, Silla University)
Publication Information
Journal of Life Science / v.15, no.6, 2005 , pp. 923-927 More about this Journal
Abstract
ln order to develop yeast cells that produce a bacteriocin on their cell surfaces, the 114 bp Leucocin A gene with stop codon was ligated into pYDl, an yeast vector. The recombinant DNA, pYDl-LeucoA was used to transform yeast (Saccharomyces cerevisiae) cells. Yeast cells harboring pYDl-LeucoA showed antibacterial activity against Bacillus subtilis. To confirm these bacteriocidal yeast cells possess the Leucocin A gone, PCR was performed with plasmid prepared from transformed yeast cells as a template and two Leucocin A-specific primers. In this study, bacteriocidal yeast cells that can be used as an antibiotic or a food preservative were developed.
Keywords
yeast; Leucocin A; bacteriocin; Saccharomyces cerevisiae;
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