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http://dx.doi.org/10.5352/JLS.2005.15.6.909

Biosenesis of Epstein-Barr Virus MicroRNAs in B Cells  

Kim Do Nyun (Research Institute of Immunobiology, Department of Biomedical Sciences, Catholic University of Korea)
Oh Sang Taek (Research Institute of Immunobiology, Department of Biomedical Sciences, Catholic University of Korea)
Lee Jae Myun (Department of Microbiology, Yonsei University)
Lee Won-Keun (Division of Bioscience and Bioinformatics, Myongji University)
Lee Suk Kyeong (Research Institute of Immunobiology, Department of Biomedical Sciences, Catholic University of Korea)
Publication Information
Journal of Life Science / v.15, no.6, 2005 , pp. 909-915 More about this Journal
Abstract
We investigated microRNA (miRNA) biogenesis of Epstein-Barr virus (EBV) which is the first virus shown to produce viral miRNAs. As expected, expression of all the reported EBV miRNAs were detected by Northen blot in an EBV-infected B cell line, B95-8; BHRF1-1, BHIU1-2, BHRF1-3, BART1, and BART2. The putative EBV pri-miRWAs and pre-miRNAs predicted from the known mature EBV miRNA sequences were detected by RT-PCR in B95-8 cells. Many animal miRNA genes exist as clusters of 2-7 genes and they are expressed polycistronically. As the EBV miRNAs are clustered in two regions of the EBV genome, we examined whether these clustered EBV miRNA genes are also expressed polycistronically. A long polycistronic transcript with the expected size (1602 bp) corresponding to the BHRF1-1~BHRF1-2~BHRF1-3 was amplified. However, any polycistronic transcript containing both BART1 and BART2 was detectable in B95-8. These results suggest that EBV miRNAs may be processed in a similar way with animal miRNAs and that some of the clustered EBV miRNAs can be transcribed polycistronically.
Keywords
EBV; miRNA; biogenesis; polycistronic;
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