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http://dx.doi.org/10.5352/JLS.2005.15.5.679

Isolation and Characterization of Biosurfactant from Bacillus atrophaeus DYL,-130  

Kim Sun-Hee (Department of Biotechnology, Faculty of Natural Resources and Life Science Dong-A University)
Lee Sang-Cheol (Department of Biotechnology, Faculty of Natural Resources and Life Science Dong-A University)
Park In-Hye (Department of Biotechnology, Faculty of Natural Resources and Life Science Dong-A University)
Yoo Ju-Soon (Department of Biotechnology, Faculty of Natural Resources and Life Science Dong-A University)
Joo Woo-Hong (Dept. of biology, Chang Won University)
Hwang Cher-Won (Institute of Bioscience and Technology, Handong Global University)
Choi Young-Lark (Department of Biotechnology, Faculty of Natural Resources and Life Science Dong-A University)
Publication Information
Journal of Life Science / v.15, no.5, 2005 , pp. 679-684 More about this Journal
Abstract
The objective of this study was investigate the characteristic of biosurfactant produced from the iso-lated strain. The strain was isolated from soli samples of Duck-Yu Mountain and it was identified as Bacillus atrophaeus DYL-130 by 16S rDNA and gyrA gene nucleotide sequence analysis. The surface ten-sion of culture filtrate of Bacillus atrophaeus DYL-130 decreased to 28 mN/m and its biosurfactant con-centration was determined by diluting the culture filtrate until the critical micelle concentration (CMC). The emulsifying activity and stability of crude biosurfactant was measured by using water-immiscible hydrocarbons and oils as substrate. The biosurfactant was purified by affinity chromatography and the surface activity of purified biosurfactant was measured by drop-collapsing method and it could be effectively emulsify toluene.
Keywords
Biosurfactant; drop-collapsing method; Affinity chromatography; surface-activity.;
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