Browse > Article
http://dx.doi.org/10.5352/JLS.2005.15.4.641

Effect of Growth Conditions in the Attachment of Salmonella typhimurium to the Host Cells  

Kim, Young-Hee (Division of Biological Sciences, Pusan National University)
Kim, Sam-Woong (Division of Biological Sciences, Pusan National University)
Kang, Ho-Young (Division of Biological Sciences, Pusan National University)
Publication Information
Journal of Life Science / v.15, no.4, 2005 , pp. 641-646 More about this Journal
Abstract
An approximately 10-fold higher level of adherence of Salmonella typhimurium strain TML to Int-407 cells was observed with organisms grown in Luria broth or in high-iron containing medium than those grown in low-iron containing medium. Iron specifically enhanced adherence, while other cations such as calcium, cobalt, copper, potassium, magnesium and manganese did not. It was suggested that iron did not act as a passive ligand - probably it stimulated production of bacterial factors necessary for adherence. A similar pattern of iron modulation of adhesiveness was also seen in Salmonella mutants with single or different combinations of multiple mutations in genes encoding the mannose sensitive hemagglutinin (type 1 fimbriae), mannose resistant hemagglutinin and flagellum. The adhesiveness of an isogenic fur mutant was modulated by iron in a manner similar to the wild-type strain, suggesting that iron modulation of adherence is independent of the fur gene product.
Keywords
iron replete medium; S. typhimurium; Int-407 cell; adherence;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Jones, R. L., C. M. Peterson, R. W. Grady, T. Kumbaraci, A. Cerami, and J. H. Graziano. 1977. Effects of iron chelators and iron overload on Salmonella infection. Nature (Lond) 267, 63-65   DOI   ScienceOn
2 Lockman, H. A., and R. Curtiss III, 1990. Salmonella typhimurium mutants lacking flagella or motility remain virulent in BALB/c mice. Infect. Immun. 58, 137-143
3 Lockman, H. A., and R. Curtiss III, 1992. Virulence of nontype-1 fimbriated and nonfimbriated nonflagellated Salmonella typhimurium mutants in murine typhoid fever. Infect. Immun. 60, 491-496
4 McDougall,S., and J. B. Neilands. 1984. Plasmid- and chromosome-coded aerobactin synthesisin enteric bacteria: insertion sequences flank operon in plasmid-mediated system. J. Bacteriol. 159, 300-305
5 Pollack, J. R, and J. B. Neilands. 1970. Enterobactin, an iron transport compound for Salmonella typhimurium. Biochem. Biophys. Res. Commun. 38, 989-992   DOI   ScienceOn
6 Puschmann, M., and A. M. Ganzoni. 1977. Increased resistance of iron-defficient mice to Salmonella infection. Infect. Immun. 17, 663-664
7 Schmieger, H. 1972. Phage P22-mutants with increased or decreased transduction abilities. Mol. Gen. Genet. 119, 75-88   DOI   ScienceOn
8 Sahel, I., J. L. Puente, W. J. Murray, J. Vuopio-Varkila, and G. K. Schoolnik, 1993. Cloning and characterization of the bundle-forming pilin gene of enteropathogenic Escherichia coli and its distribution in Salmonella serotypes. Mol. Microbiol. 7, 563-575   DOI   ScienceOn
9 Weinberg, E. D. 1984. Iron withholding: a defense against infection and neoplasia. Physiol. Rev. 64, 65-102
10 Doran, J. L., S. K. Collinson, J., Burian, G. Sarlos, I. C. D. Todd, C. K. Munro, C. M. Kay, P. A. Banser, P. I. peterkin, and W. W. Kay. 1993. DNA-based diagnostic tests for Salmonella species targeting agfA, the structural gene for thin, aggregative fimbriae. J. Clin. Microbiol. 31, 2263-2273
11 Ernst, J. F., R L. Benett, and L. I. Rothfield. 1978. Constitutive expression of the iron-enterochelin and ferrichrome uptake systems in a mutant strain of Salmonella typhimurium. J. Bacteriol. 135, 928-934
12 Douce, G. R, I. I. Amin, and J. Stephen. 1991. Invasion of HEp-2 cells by strains of Salmonella typhimurium of different virulence in relation to gastroenteritis. J. Med. Microbiol. 35, 349-357   DOI
13 Friedrich, M. J., N. E. Kinsey, J. Vila, and R. J. Kander. 1993. Nucleotide sequence of a 13.9 kb segment of the 90 kb virulence plasmid of Salmonella typhimurium: the presence of fimbrial biosynthetic genes. Mol. Microbiol. 8, 543-558   DOI   ScienceOn
14 Ernst, R. K., D. M. Dombroski, and J. M. Merrick. 1990. Anerobiosis, type 1 fimbriae and growth phase are factors that affect invasion of HEp-2 cells by Salmonella typhimurium. Infect. Immun. 58, 2014-2016
15 Galan, J. E. and R Curtiss Ill, 1989. Cloning and molecular characterization of genes whose products allow Salmonella typhimurium to penetrate tissue culture cells. Proc. Natl. Acad. Sci. USA. 86, 6683-6387
16 Green, R, R. Charlton, H. Seftel, T. Bothwell, F. Mayet, B. Adams, C. Flinch, and M. Layrisse. 1968. Body iron excretion in man. Am. J. Med. 45, 336-353   DOI   ScienceOn
17 Jones, B. D., C. A. Lee, and S. Falkow. 1992. Invasion by Salmonella typhimurium is affected by the direction of flagellar rotation. Infect. Immun. 60, 2475-2480
18 Jones, G. W., and L. A. Richardson. 1981. The attachment to and invasion of Hela cells by Salmonella typhimurium: the contribution of mannose-sensitive and mannose-resistant haemagglutinating activities. J. Gen. Microbiol. 127, 361-370
19 Jones, G. W., D. K. Svinarich, and H. J. Whitfield. 1982. Association of adhesive, invasive, and virulent phenotypes of Salmonella typhimurium with autonomous 60-megadalton plasmids. Infect. Immun. 38, 476-486
20 Baumler, A. J., and F. Heffron. 1995. Identification and sequence analysis of 1pfABCDE, a putative fimbrial operon of Salmonella typhimurium. J. Bacteriol. 177, 2087-2097
21 Davenport, H. W. 1982. Physiology of the Digestive Tract. pp. 181-194. Chicago: Yearbook Medical Publishers Inc
22 Bagg, A., and J. B. Neilands. 1987. Molecular mechanism of regulation of siderophore-mediated iron assimilation. Microbiol. Rev. 51, 509-518