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http://dx.doi.org/10.5352/JLS.2005.15.4.600

Developmental Expression of Neurofilament 3 (NF-M) in the Cultured Rat Cortical Neurons  

Jung Jae-Seob (Department of Anatomy, College of Medicine, Dongguk University)
Cho Sun-Jung (Department of Microbiology, College of Natural Sciences, Kyungpook National University)
Jin IngNyol (Department of Microbiology, College of Natural Sciences, Kyungpook National University)
Jung Seung Hyun (Department of Internal Medicine, College of Oriental Medicine, Dongguk University)
Moon Il Soo (Department of Anatomy, College of Medicine, Dongguk University)
Publication Information
Journal of Life Science / v.15, no.4, 2005 , pp. 600-606 More about this Journal
Abstract
Neurofilament (NF) proteins constitute the major intermediate filament type in adult neurons. They are made up by the copolymerization of the neurofilament light (NF-L, 61 kDa), medium (NF-M, 90kDa), and heavy (NF-H, 115 kDa) proteins. Although neurofilaments play a crucial .ole in neuronal growth, organization, shape, and plasticity, their expression pattern and cellular distribution in the developing neurons remain unknown. In this study, we have produced a rabbit polyclonal antibody specific to NF-M and investigated expression of NF-M in cultured cortical neurons. Immunostaining of 12 and 24 h cultures revealed strong expression of NF-M in axonal growth cone and in the region of a soma toward the axon. Doublestaining of 4 and 14 DIV corical neurons with NF-M and PSD95 antibodies revealed that both axon and dendrites were stained intensely with NF-M antibody, and that NF-M immunostaining along dendrites is often punctate and colocalize with PSD95 puncta, indicating that the puncta represent postsynaptic spines. Presence of NF-M in the postsynaptic spine was also indicated by immunoblot analysis of the postsynaptic density fraction. Taken together, our results show intensive targeting of NF-M into axons in the early axonal development, and into spines in mature neurons, indicating its important functions in axon and spine development.
Keywords
cortical culture; growth cone; immunocytochemistry; neurofilament; spine;
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