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http://dx.doi.org/10.5352/JLS.2005.15.4.528

Antioxidant Activity and Identification of Lunasin Peptide as an Anticancer Peptide on Growing Period and Parts in Pepper  

Kwon Ki Soo (Andong Agricultural Technology and Extension Center)
Park Jae Ho (School of Bioresources, Andong National University)
Kim Dae Seop (School of Bioresources, Andong National University)
Jeong Jin Boo (School of Bioresources, Andong National University)
Sim Young Eun (Andong Agricultural Technology and Extension Center)
Kim Mi Suk (School of Bioresources, Andong National University)
Lee Hee Kyung (School of Bioresources, Andong National University)
Chung Gyu Young (School of Bioresources, Andong National University)
Jeong Hyung Jin (School of Bioresources, Andong National University)
Publication Information
Journal of Life Science / v.15, no.4, 2005 , pp. 528-535 More about this Journal
Abstract
The non-enzymatic anti-oxidants and lunasin peptide from the extracts of the pepper were examined in order to utilize the discovery in natural products as cancer chemopreventive agents. The DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical scavenging activity on the fruit parts of the pepper was higher than that of the seed, but the difference was low. The Inhibition activity of xanthine/ xanthine oxidase in extracts of the seed was higher than that of the fruit and that of the seed on 20 days after flowering was the highest at the growing period. These were identified as fatty acids and phenolic compounds such as 1-eicosanol, palmitic acid, linoleic acid, linolenic acid and benzonitrile. The contents of fatty acids and phenolic compounds increased according to the time passing at the growing period. Peroxidase (POD) activity of the fruit at middle stage was high than that of other growing stages and that of the seed was the highest at later growing period. Though superoxide dismutase (SOD) activities in fruit were hish by passage of Slowing stage, the activity in seed was low. Lunasin was searched from seeds of the peppers by coomassie blue staining and western blot among them and we just found lunasin peptide from extracted protein of the pepper by western blot. In addition, we observed the contents of lunasin after flowering and confirmed to appear the lunasin at 35 days after flowering. We confirmed that lunasin is complex protein of maturing seeds. 100nM lunasin peptide in pepper showed inhibition effect on colony formation in $2\~12$ cells.
Keywords
DPPH; Xanthin/xanthine oxidase; POD; SOD;
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