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http://dx.doi.org/10.5352/JLS.2005.15.3.461

Protective Effect of DWP-04 Against Hepatotoxicity Induced by D-galactosamine  

Lee Jung-Hee (College of Pharmacy, Kyungsung University)
Chi Sang Cheol (College of Pharmacy, Sungkyunkwan University)
Kim Seok-Hwan (Department of Food and Nutrition, Dong-A University)
Shin Young-Ho (Dae Woo Pharmaceutical Ind. Co.)
Choi Jongwon (College of Pharmacy, Kyungsung University)
Publication Information
Journal of Life Science / v.15, no.3, 2005 , pp. 461-467 More about this Journal
Abstract
This study was conducted to investigate the biological activity and hepatoprotective effect of DWP-04 [DDB : selenium yeast: glutathione (31.1 : 6.8 : 62.1(w/w/w)] in D-galactosamine (GaIN) intoxicated rats. The DWP-04 (50, 100 or 200 mg/kg) or its vehicle was orally administered everyday before the start of GaIN injection (400 mg/kg, ip) for two weeks and animal decapitated for 24 hrs after GaIN­injected. The activities of serum enzymes, markers of liver function, were increased in the GaIN group compared to normal group and significantly lowered in the DWP-04 pretreated group than in the GaIN group. Hepatic lipid peroxide level and activities of phase 1 enzymes were significantly higher than those of GaIN group compared to normal group and lower in the DWP-04 pretreated group than in the GaIN group, and phase II enzyme activities in liver were lower in the GaIN group than in the normal group and were increased in the DWP-04 pretreated group than in the GaIN group. Total hepatic glutathione content and glutathione biosynthesis enzymes were lower in the GaIN group than in the normal group and were increased in the DWP-04 pretreated group than in the GaIN group. Therefore, the current results indicated that DWP-04 administration alleviated the GaIN-induced adverse effect through enhancing the antioxidant enzyme activities.
Keywords
DWP-04; biphenyldimethyl dicarboxylate; selenium yeast; glutathione; hepatotoxicity; D-galactosamine;
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