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http://dx.doi.org/10.5352/JLS.2005.15.2.215

Evaluation of Safety with Gamgung-tang Using Ames and umu Assays  

Shon Yun Hee (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
Kim Cheorl Ho (Department of Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University)
Nam Kyung Soo (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
Publication Information
Journal of Life Science / v.15, no.2, 2005 , pp. 215-219 More about this Journal
Abstract
Gamgung-tang (GGT) that is included in Gamdu-tang (consists of Glycyrrhizae Radix, black beans) and Gunggui-tang(consists of Angelicae Radix and Cnidii Rhizoma) showed therapeutic effect of autoimmume thyroiditis in the previous reports. GGT was tested for the safety using Ames and umu gene expression mutagenicity tests. In Ames test, Salmonella typhimurium TA98 and TA100 were used to identify mutagenic property, and the number of histidine revertants was measured. In SOS umu test, Salmonella typhimurium TA1535 containing plasmid pSK1002 was used as a test strain, and we monitored the levels of umu operon expression by measuring the $\beta-galactosidase$ activity. Mutagenic activity in any assays we tested was not found. After treating S-9 mixture with GGT, mutagenic activity was also not found. The results of this study suggested that there was no DNA damage and mutagenicity of GGT.
Keywords
Gamgung-tang; mutagenicity; Ames test; SOS umu test;
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