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http://dx.doi.org/10.5352/JLS.2005.15.2.161

Molecular Typing of Legionella pneumophila Isolated in Busan, Using PFGE  

Park Eun-Hee (Busan Metropolitan City Institute of Health & Environment)
Kim Mi-Hee (Busan Metropolitan City Institute of Health & Environment)
Kim Joung-A (Busan Metropolitan City Institute of Health & Environment)
Han Nan-Sook (Busan Metropolitan City Institute of Health & Environment)
Lee Ju Hyeoun (Busan Metropolitan City Institute of Health & Environment)
Min Sang Gi (Busan Metropolitan City Institute of Health & Environment)
Park Yon Koung (Busan Metropolitan City Institute of Health & Environment)
Jin Seong Hyun (Busan Metropolitan City Institute of Health & Environment)
Jeong Gu Young (Busan Metropolitan City Institute of Health & Environment)
Bin Jae Hun (Busan Metropolitan City Institute of Health & Environment)
Publication Information
Journal of Life Science / v.15, no.2, 2005 , pp. 161-168 More about this Journal
Abstract
In this study, we did the molecular typing of 39 environmental Legionella pneumophila serogroup 1 isolates collected from 2001-2003 in Busan using the pulsed-filed gel electrophoresis (PFGE). PFGE of SfiI fragments were divided into 10 pulsotypes $(A\~J)$, corresponding to $<65\%$ similarity and a subtype within each pulsotype was characterized by $>84\%$ similarity. The major cluster was pulsotype E $(46.2\%)$, which included 18 isolates and was divided into 4 subtypes $(E1\~E4)$. PFGE of NotI fragments were divided into 8 pulsotypes $(a\~h)$, corresponding to $<60\%$ similarity and a subtype within each pulsotype was characterized by $100\%$ similarity. The major cluster was pulsotype f $(38.5\%)$, which included 15 isolates. The ATCC type strain L. pneumophila serogroup 1 was identified as a different molecular pulsotype compare to the Busan isolates. It is possible that L. pneumophila serogroup 1 isolated in Busan with specific DNA pattern is comparable with those isolation in other cities in Korea.
Keywords
Legionella pneumophila serogroup 1; PFGE; Busan;
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